Paenibacillus lupini sp. nov., isolated from nodules of Lupinus albus

Abstract

A bacterial strain designated RLAHU15T was isolated from root nodules of Lupinus albus in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Paenibacillus, with its closest relatives being Paenibacillus catalpae D75 T , Paenibacillus glycanilyticus DS-1 T , Paenibacillus endophyticus PECAE04 T and Paenibacillus xinjiangensis B538 T with 98.8 %, 98.9 %, 97.4 % and 97.4 % similarity, respectively. DNA-DNA hybridization studies showed values lower than 45 % between the strain RLAHU15 T and any of these species.The isolate was a Gram-stain positive, motile and sporulating rod. Catalase activity was weak and oxidase activity was positive. Casein and starch were hydrolysed but gelatin was not. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the only menaquinone detected and anteiso-C 15 : 0 and iso-C 16 : 0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and an unidentified lipid. meso-Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 54.4 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RLAHU15 T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus lupini sp. nov. is proposed. The type strain is RLAHU15 T (5LMG 27296 T 5CECT 8235 T ).

Paenibacillus periandrae sp. nov., isolated from nodules of Periandra mediterranea

“…The major fatty acid is anteiso-C 15 : 0 and meso-diaminopimelic acid is commonly present in the peptidoglycan (Priest, 2009). This genus is ubiquitous in nature and several species have been originally found in legume nodules (Valverde et al, 2010;Carro et al, 2013Carro et al, , 2014.…”

Paenibacillus medicaginis sp. nov. a chitinolytic endophyte isolated from a root nodule of alfalfa (Medicago sativa L.)

“…Strain CC-Alfalfa-19 T contained MK-7 as the only respiratory quinone. Species of the genus Paenibacillus have been reported to possess MK-7 as the sole (Carro et al , 2014) or predominant respiratory quinone (Carro et al , 2013; Glaeser et al , 2013; Kämpfer et al , 2012; Kim et al , 2004; Kittiwongwattana & Thawai, 2014; Shida et al , 1997; Valverde et al , 2010). The predominant diamino acid detected in the cell-wall peptidoglycan of strain CC-Alfalfa-19 T was meso -diaminopimelic acid, which is in line with the data reported previously for species of the genus Paenibacillus (Carro et al , 2013, 2014; Kämpfer et al , 2012; Kim et al , 2004; Kittiwongwattana & Thawai, 2014; Priest, 2009; Shida et al , 1997; Valverde et al , 2010).…”

Paenibacillus kyungheensis sp. nov., isolated from flowers of magnolia

“…Species of the genus Paenibacillus are either Gram-stain-positive or variable, facultatively anaerobic or strictly aerobic, produce ellipsoidal endospores, and are non-pigmented, rod-shaped and motile, with a DNA G+C content of 40 to 54 mol% (Ash et al , 1993; Shida et al , 1997a,b; Slepecky & Hemphill, 1992; Claus & Berkeley, 1986). Members of the genus are widespread micro-organisms commonly isolated from various sources, including the rhizosphere of trees (Jiang et al , 2015; Jin et al , 2011b), γ-irradiated Antarctic soil (Dsouza et al , 2014), duckweed (Kittiwongwattana & Thawai, 2015), nodules of Lupinus albus (Carro et al , 2014), tidal flats (Wang et al , 2012), water springs (Tang et al , 2011), rice seeds (Liu et al , 2010), iron mineral soil (Cao et al , 2015) and necrotic wounds (Glaeser et al , 2013). The cell wall peptidoglycan of members of the genus Paenibacillus is meso -diaminopimelic acid, and menaquinone-7 (MK-7) is the predominant menaquinone.…”