Padronização de bioensaios para detecção de compostos alelopáticos e toxicantes ambientais utilizando alface

Simões, Mateus Salomão; Madail, Rafael Hansen; Barbosa, Sandro; Nogueira, Marina de Lima

doi:10.5007/2175-7925.2013v26n3p29

Cited by 14 publications

(9 citation statements)

References 14 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The main advantages of using Lactuca sativa L. as a plant model lies in the sensitivity of the species even in low concentrations of tested compounds, as well as the low research cost. In addition, Lactuca sativa has other peculiarities that favor its use-fast germination in approximately 24 hours, linear growth over a wide range of pH variation, low sensitivity to osmotic potentials, establishment of plant with approximately 21 days, small number of chromosomes (2n = 2x = 18), and presence of large chromosomes [22,23]. The last two characteristics cited are advantageous for cytogenetic analysis [18].…”

Section: Introductionmentioning

confidence: 99%

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Govêa

Pereira

Assis

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

Coumarins are widely distributed substances in plant species that promote phytotoxic effects, allowing them to be exploited as herbicides less harmful to the environment, since many invasive species have demonstrated resistance to commercially available products. The derived coumarins used in this study had not been tested in plant models and their effect on plants was unknown. The objective of this study was to evaluate the phytotoxic action of these coumarins in bioassays with Lactuca sativa L., in order to select the most responsive substance whose toxicity was best elucidated by chromosomal complement and enzymatic antioxidant metabolism studies. From the phytotoxicity assays, coumarin 8-methoxy-2-oxo-6-(prop-2-en-1-yl)-2H-chromene-3-carboxylic acid (A1), reported here for the first time, was selected as the most responsive and caused a reduction in the following parameters: number of normal seedlings, fresh biomass, root length and shoot length. Subsequent studies demonstrated that this coumarin is cytogenotoxic due to damage caused to the cell cycle and the occurrence of chromosomal abnormalities. However, it did not interfere with antioxidant enzyme activity and did not cause lipid peroxidation. The changes caused by coumarin A1 described herein can contribute to better understanding the allelochemical actions of coumarins and the potential use of these substances in the production of natural herbicides.

show abstract

Section: Introductionmentioning

confidence: 99%

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Govêa

Pereira

Assis

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…The phytotoxicity test was carried out as described by Ribeiro et al (2012) and Simões et al (2013), using 3 replicates of 50 seeds of lettuce (Lactuca sativa cv. Grand rapids), in Petri dishes containing 5 ml of the different concentrations (0, 5, 10, 20 e 40 mg.mL -1 ) of ethanolic and aqueous extracts of all Schinus molle structures tested.…”

Section: Phytotoxic Testmentioning

confidence: 99%

The species used in urban afforestation can present phytotoxicity - a case of study of <i>Schinus molle</i> L.

et al. 2021

View full text Add to dashboard Cite

O objetivo deste estudo foi estudar a fitotoxicidade e citotoxicidade de extratos etanólico e aquoso das diferentes estruturas de Schinus molle L., uma espécie ornamental, comumente usada em arborização urbana no Brasil, utilizando sementes de alface (Lactuca sativa L.) como planta modelo. Os extratos vegetais foram preparados a partir de amostras de folhas, inflorescências, frutos verdes e maduros coletados em Alfenas, Minas Gerais, Brasil. Os parâmetros avaliados foram germinação (G), índice de velocidade de germinação (GVI), comprimento radicular (RE), biomassa fresca (FB), biomassa seca (DB), porcentagem de plântulas (PS), índice mitótico (MI) e anomalias cromossômicas (CA). De acordo com os resultados, para o extrato aquoso, encontramos interações entre as estruturas e as concentrações utilizadas em relação a G, GVI, RE, DB, e CA. Não houve diferença significativa entre as estruturas vegetais em relação à porcentagem de plântulas normais, exceto para as concentrações. No entanto, para todos os parâmetros foi encontrada diferença significativa em relação ao extrato etanólico, exceto para anormalidades cromossômicas. O aumento da concentração leva à redução de todos os parâmetros avaliados, mostrando o efeito concentração-dependente. Os extratos etanólico e aquoso de Schinus molle mostraram efeito fitotóxico, indicando a necessidade de reavaliação e cuidado no uso dessa espécie para o reflorestamento.

show abstract

“…As substrate, filter paper discs impregnated with 2 mL of each extract were put into 9 cm Petri dishes. After all solvent evaporation, 2 mL of sterile water were added to humidify the substrate (17) .…”

Section: Germination Bioassaymentioning

confidence: 99%

Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae)

2020

View full text Add to dashboard Cite

The allelopathic action can be verified by the presence of phytotoxic substances, molecules that are directed to the agribusiness sector. The objective of this study was to evaluate the chemical composition of the methanolic extracts from Colletotrichum dianesei and Xylaria sp., and phytotoxic potential on Lactuca sativa. The fungi were grown in Sabouraud liquid medium supplemented with 0.2% yeast extract, under stirring. The mycelia were subjected to solvent extraction in order of increasing polarity, but methanol extracts were used in this investigation. The allelopathic activity was evaluated at a concentration of 1000 µg/mL in quadruplicate, evaluating: percentage and speed of germination, and growth of lettuce seedlings under laboratory conditions. C. dianesei extract reduced germination percentage by 36% and delayed germination speed by 63.8%, while Xylaria sp. didn't interfere with the germination percentage, it only delayed the germination rate by 43.9%. Root growth was negatively affected in 30.5% and 51.1% under the influence of extracts from Xylaria sp. and C. dianesei, there was also a reduction of 33.1 and 31.8% in the aerial part length, respectively. The thin layer chromatography showed the presence of aromatic substances and terpenes in both extracts, and alkaloids only in C. dianesei extract. The 1H nmr spectra showed signals between 6 and 8.5 ppm (aromatic substances), being relatively more intense for Xylaria sp., signals between 3 and 4 ppm (sugars, showing anomers only for Xylaria sp.) and methyl groups between 0.7 and 1.3 ppm, suggesting terpenes. The results suggest that the fungal species evaluated have phytotoxic potential, and produce terpenes and aromatic substances.

show abstract

Padronização de bioensaios para detecção de compostos alelopáticos e toxicantes ambientais utilizando alface

Cited by 14 publications

References 14 publications

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

The species used in urban afforestation can present phytotoxicity - a case of study of <i>Schinus molle</i> L.

Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae)

Contact Info

Product

Resources

About