Paclitaxel-PHBV nanoparticles and their toxicity to endometrial and primary ovarian cancer cells

Vilos, Cristián; Morales, Francisco Álvarez; Solar, Paula; Herrera, Natalia; González‐Nilo, Fernando; Aguayo, Daniel; Mendoza, Hegaly; Comer, Jeffrey; Bravo, María Loreto; González, Pamela; Kato, Sumie; Cuello, Mauricio; Alonso, Catalina; Bravo, Erasmo; Bustamante, Eva; Owen, Gareth I.; Velásquez, Luís

doi:10.1016/j.biomaterials.2013.02.034

Cited by 91 publications

(59 citation statements)

References 35 publications

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“…Table 1 describes the diameter (nm), polydispersity index (PdI), and the Zeta Potential (mV) obtained of NPs, and Additional file 1: Figure S1 exhibits a graphical representation of the data analyzed statistically. The size presented in all formulations of NPs was homogenous with a diameter of ~200 nm, stable in time (Additional file 2: Figure S2), and similar to the size of paclitaxel-loaded PHBV nanoparticles synthesized previously by our group [32]. …”

Section: Resultssupporting

confidence: 74%

Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines

et al. 2017

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BackgroundNanotechnology is a science that involves imaging, measurement, modeling and a manipulation of matter at the nanometric scale. One application of this technology is drug delivery systems based on nanoparticles obtained from natural or synthetic sources. An example of these systems is synthetized from poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which is a biodegradable, biocompatible and a low production cost polymer. The aim of this work was to investigate the uptake mechanism of PHBV nanoparticles in two different epithelial cell lines (HeLa and SKOV-3).ResultsAs a first step, we characterized size, shape and surface charge of nanoparticles using dynamic light scattering and transmission electron microscopy. Intracellular incorporation was evaluated through flow cytometry and fluorescence microscopy using intracellular markers. We concluded that cellular uptake mechanism is carried out in a time, concentration and energy dependent way. Our results showed that nanoparticle uptake displays a cell-specific pattern, since we have observed different colocalization in two different cell lines. In HeLa (Cervical cancer cells) this process may occur via classical endocytosis pathway and some internalization via caveolin-dependent was also observed, whereas in SKOV-3 (Ovarian cancer cells) these patterns were not observed. Rearrangement of actin filaments showed differential nanoparticle internalization patterns for HeLa and SKOV-3. Additionally, final fate of nanoparticles was also determined, showing that in both cell lines, nanoparticles ended up in lysosomes but at different times, where they are finally degraded, thereby releasing their contents.ConclusionsOur results, provide novel insight about PHBV nanoparticles internalization suggesting that for develop a proper drug delivery system is critical understand the uptake mechanism.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0241-6) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 74%

Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines

et al. 2017

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“…In this study, Formation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) particles 7 nanoparticles, in which the EE is known to be dependent on chemical structure, molecular weight and hydrophobicity of the polymers (Yeo and Park 2004). PHBV nanoparticles have been recently used for encapsulation of paclitaxel and the loading capacity was found to be between 35 and 40% for this hydrophobic drug by Vilos et al (2013). In another study by Masood et al (2013), high encapsulation of ∼ 45% was obtained for the hydrophobic Ellipticine anticancer drug.…”

Section: Solvent Typementioning

confidence: 98%

“…Th e chemical structure and physicochemical properties of PHBV are similar to the commonly studied polyester poly (lactide-co-glycolide) (PLGA) (Vroman and Tighzert 2009). On the other hand, it degrades at a much slower rate than PLGA (Yilgor et al 2010) and it is a more cost eff ective alternative due to its biotechnological production (Vilos et al 2013). Although there are plenty of studies on the production of PLGA particles as carrier systems, few studies have been reported on the physicochemical properties of PHBV particles prepared using diff erent process parameters (Pich et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV) is a microbial polyester synthesized by various microorganisms. It has been reported that PHBV shows good biocompatibility and has been attractive as a biomaterial for tissue engineering (Zhu et al 2009) and particle based drug delivery systems (Vilos et al 2013, Masood et al 2013. Th e chemical structure and physicochemical properties of PHBV are similar to the commonly studied polyester poly (lactide-co-glycolide) (PLGA) (Vroman and Tighzert 2009).…”

Section: Introductionmentioning

confidence: 99%

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Effect of emulsification-diffusion parameters on the formation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) particles

Göz

Karakeçili

2014

Artificial Cells, Nanomedicine, and Biotechnology

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The aim of this work was to evaluate the effect of various production parameters on the formation and particle size of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV particles prepared by the emulsification-diffusion technique. The increase in homogenization time and speed caused a decrease in particle size. No particle formation was observed below 2% (w/v) PHBV in the organic phase. Smaller particle size and narrower size distribution were observed when polyvinyl alcohol (PVA) was used as a stabilizer, when compared to didodecyldimethylammonium bromide. Submicron particles of 531 ± 150 nm size were obtained with 2% (w/v) PVA at 17 500 rpm and 15 min homogenization conditions with dichloromethane as the organic solvent.

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“…PHBV nanoparticles were prepared by an emulsification/ solvent evaporation technique adapted from previously described methods (Vilos et al, 2013). Briefly, 10 mg of PHBV was dissolved in 10 mL of chloroform to obtain 0.1% (w/v) organic phase.…”

Section: Preparation Of Phbv Nanoparticlesmentioning

confidence: 99%

Targeted delivery of etoposide to osteosarcoma cells using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles

Alp¹,

Çırak²,

Demirbilek³

et al. 2017

Turk J Biol

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Folic acid (FA)-functionalized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles were prepared to enhance the delivery efficiency of the anticancer drug etoposide for the clinical treatment of osteosarcoma. PHBV nanoparticles were synthesized by emulsification/solvent evaporation technique and obtained in the size range of 200-250 nm and zeta potential range of -21 and -27 mV. Encapsulation efficiency and in vitro drug release were studied. The cytotoxic, apoptotic, and necrotic effects of PHBV nanoparticles were also investigated using Saos-2 osteosarcoma cells. The results obtained in this study demonstrate that etoposideloaded and FA-functionalized PHBV nanoparticles can be successfully used for targeted treatment of osteosarcoma.

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Paclitaxel-PHBV nanoparticles and their toxicity to endometrial and primary ovarian cancer cells

Cited by 91 publications

References 35 publications

Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines

Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines

Effect of emulsification-diffusion parameters on the formation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) particles

Targeted delivery of etoposide to osteosarcoma cells using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles

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