Paclitaxel‐loaded silk fibroin nanospheres

Abstract

Silk fibroin is a very promising biomedical material because of its renewability, nontoxicity, biocompatibility, and biodegradability. On the basis of a simple and mild method for the preparation of silk fibroin nanospheres with controllable size, the authors developed earlier, anti-cancer drug paclitaxel (PTX)-loaded silk fibroin nanospheres ranging from 270 to 520 nm were produced accordingly. The drug loading, encapsulation efficiency, and released property of PTX-loaded silk fibroin nanospheres are depended on the silk fibroin concentration and initial PTX-loading capacity. The maximum drug loading is about 6.9% and the release time of such a kind of nanospheres is over 9 days. The release time of PTX-loaded silk fibroin nanospheres can be as long as 2 weeks when the drug loading is about 3.0%. All these results imply that such a kind of biomacromolecule-based anti-cancer drug nanocarrier has a great potential for chemotherapy in clinical applications.

Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats

“…The RAW 264.7 cells were seeded onto 96-well plates, at a density of 5×10 5 cells per well, and grown until confluence. They were cultured for 2 hours with each concentration of the nanoparticles (100, 250, and 500 μg/mL) (alone or loaded with resveratrol) and then stimulated with lipopolysaccharide (LPS) (100 ng/mL); similarly, positive and negative controls were also used, including three concentrations of resveratrol (12,20, and 60 μg/mL) -which correspond to the maximal release that could be obtained with each concentration of the RL-FNPs assayed. After 20 hours, the supernatants were collected and centrifuged at 10,000 g for 5 minutes: the nitrite levels, as an indirect marker of inducible nitric oxide synthase (iNOS) activity, were measured using the Griess assay.…”

“…7 In addition, fibroin can be elaborated as micro-and nanoparticles, with excellent properties as vectors for drug delivery. [8][9][10] Many compounds can be encapsulated in fibroin particles, such as proteins, like insulin; 11 antitumorals, like paclitaxel; 12 and curcumin. 13 In addition, fibroin is highly biocompatible, and its degradation products from the action of proteases are peptides that are easily reabsorbed by the tissues.…”

Doxorubicin-Loaded Silk Fibroin Nanospheres

“…2.1 负载阿霉素的丝蛋白纳米微球的制备 在作者先前采用丝蛋白纳米微球包裹疏水性药物 紫杉醇和亲水性药物氟脲苷时, 两种药物都可以和丝蛋 白乙醇溶液均匀混合, 然后通过冷冻成球从而实现对药 物的有效包覆 [22,23] . [24,25] , 并可使 纳米微球"隐形", 从而避免其在达到肿瘤位点前就被 清除出去; 亦有研究表明使用聚乙二醇类聚合物能够延 长药物在体内的半衰期, 从而减少药物使用次数 [26,27] .…”

Silk fibroin nanoparticle as a novel drug delivery system

“…The maximum loading of paclitaxel in 270-to 520-nm nanospheres was about 6.9%, which was released over 9 days as reported by Chen and colleagues. The authors described that the drug loading, encapsulation efficiency, and release rate of paclitaxel-loaded SF nanospheres was highly dependent on SF concentration and the initial paclitaxel-loading capacity [244]. It is beyond doubt that combining SF with other natural and synthetic polymers could improve its structural, mechanical, and degradation properties along with its drug release profile, drug retention, and encapsulation efficiency [245,246].…”