Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats

Abstract

Purpose We aimed to evaluate the intestinal anti-inflammatory properties of silk fibroin nanoparticles, around 100 nm in size, when loaded with the stilbene compound resveratrol, in an experimental model of rat colitis. Methods Nanoparticles were loaded with resveratrol by adsorption. The biological effects of the resveratrol-loaded nanoparticles were tested both in vitro, in a cell culture of RAW 264.7 cells (mouse macrophages), and in vivo, in the trinitrobenzenesulfonic acid model of rat colitis, when administered intracolonically. Results The resveratrol liberation in 1× phosphate-buffered saline (PBS; pH 7.4) was characterized by fast liberation, reaching the solubility limit in 3 hours, which was maintained over a period of 80 hours. The in vitro assays revealed immunomodulatory properties exerted by these resveratrol-loaded nanoparticles since they promoted macrophage activity in basal conditions and inhibited this activity when stimulated with lipopolysaccharide. The in vivo experiments showed that after evaluation of the macroscopic symptoms, inflammatory markers, and intestinal barrier function, the fibroin nanoparticles loaded with resveratrol had a better effect than the single treatments, being similar to that produced by the glucocorticoid dexamethasone. Conclusion Silk fibroin nanoparticles constitute an attractive strategy for the controlled release of resveratrol, showing immunomodulatory properties and intestinal anti-inflammatory effects.

Intestinal Anti-inflammatory Effects of Outer Membrane Vesicles from Escherichia coli Nissle 1917 in DSS-Experimental Colitis in Mice

“…These regulatory effects were not accompanied by changes in ZO-1 or occludin protein levels (Buda et al, 2012). Expression of TFF-3 is downregulated in intestinal inflammation (Lozano-Pérez et al, 2014), as observed in the present study. In connection with this, here we show that EcN OMVs increase colonic expression of this bioactive peptide involved in epithelial protection and repair (Podolsky et al, 2009), and preserve the damaged colonic cytoarchitecture of the mucosa in colitic mice.…”

Protective effects of curcumin on acute gentamicin-induced nephrotoxicity in rats

“…Sirt1 activator resveratrol has been shown to attenuate the progression of nonalcoholic fatty liver disease (Faghihzadeh et al 2014) and inflammatory bowel disease (Lozano-Perez et al 2014). Considerable evidences indicated that Sirt1 is protective against a variety of kidney injury including LPS induced AKI, ischemia-reperfusion renal injury and diabetic nephropathy by suppressing kidney inflammation and down-regulating inflammatory signaling (Gao et al 2014;Hao and Haase 2010;Khader et al 2014).…”

Recent Advances on the Anti-Inflammatory and Antioxidant Properties of Red Grape Polyphenols: In Vitro and In Vivo Studies

“…In animal models of IBD, RES administration was very effective in reducing mucosal inflammation via inhibition of malondialdehyde and increase of glutathione peroxidase activities, respectively [239][240][241][242][243][244]. Furthermore, in the above models, decrease in neutrophil infiltration and proinflammatory cytokine release and increase in number of Bifidobacteria and Lactobacilli with reduction of intestinal wall fibrosis have been observed [239][240][241][242][243][244].…”