P6A-5 Real-Time Optoacoustic Imaging Using Near Infrared Absorbing Gold Nanoshells For Contrast Enhancement

Fournelle, Marc; Maass, Kirsten; Fonfara, Heinrich; Welsch, H.-J.; Hewener, Holger; Günther, Christian; Lemor, Robert

doi:10.1109/ultsym.2007.608

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Although several optical devices have been used to validate applications of gold nanoshells as viable contrast agents [3][4][5][6][7][8][9][10][11][12][13][14][15], none have focused on evaluating the effectiveness of using immunotargeted nanoshells as contrast agents for cell surface biomarkers using nonlinear excitation microscopy. Nonlinear optics has been used extensively for analyzing fluorescent signals in animal models and tissue samples [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

Bickford

Sun

et al. 2008

Nanotechnology

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We demonstrate the capability of using immunotargeted gold nanoshells as contrast agents for in vitro two-photon microscopy. The two-photon luminescence properties of different-sized gold nanoshells are first validated using near-infrared excitation at 780 nm. The utility of two-photon microscopy as a tool for imaging live HER2-overexpressing breast cancer cells labeled with anti-HER2-conjugated nanoshells is then explored and imaging results are compared to normal breast cells. Five different imaging channels are simultaneously examined within the emission wavelength range of 451-644 nm. Our results indicate that under near-infrared excitation, superior contrast of SK-BR-3 cancer cells labeled with immunotargeted nanoshells occurs at an emission wavelength ranging from 590 to 644 nm. Luminescence from labeled normal breast cells and autofluorescence from unlabeled cancer and normal cells remain imperceptible under the same conditions.

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Section: Introductionmentioning

confidence: 99%

Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

Bickford

Sun

et al. 2008

Nanotechnology

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“…Wang et al demonstrated enhanced contrast between blood vessels and the surrounding brain tissue due to GNS absorption using in vivo photoacoustic tomography (PAT) 11 . In vitro tissue phantom studies using optoacoustic imaging12 demonstrated GNS as effective absorbing agents providing enhanced contrast. Recently, researchers have taken advantage of the two-photon luminescence properties of GNS to image the 3-D distribution of GNS in tumors13 and image live breast cancer cells with immunotargeted GNS using in vitro two-photon microscopy 14.…”

Section: Introductionmentioning

confidence: 99%

Near-infrared narrow-band imaging of gold/silica nanoshells in tumors

et al. 2009

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Gold nanoshells (GNS) are a new class of nanoparticles that can be optically tuned to scatter or absorb light from the near-ultraviolet to near-infrared (NIR) region by varying the core (dielectric silica)/shell (gold) ratio. In addition to spectral tunability, GNS are inert and bioconjugatable, making them potential labels for in vivo imaging and therapy of tumors. We report the use of GNS as exogenous contrast agents for enhanced visualization of tumors using narrow-band imaging (NBI). NBI takes advantage of the strong NIR absorption of GNS to distinguish between blood and nanoshells in the tumor by imaging in narrow wavelength bands in the visible and NIR, respectively. Using tissue-simulating phantoms, we determined the optimum wavelengths to enhance contrast between blood and GNS. We then used the optimum wavelengths for ex vivo imaging of tumors extracted from human colon cancer xenograft bearing mice injected with GNS. Systemically delivered GNS accumulated passively in tumor xenografts by the enhanced permeability and retention (EPR) effect. Ex vivo NBI of tumor xenografts demonstrated heterogeneous distribution of GNS with a clear distinction from the tumor vasculature. The results of this study demonstrate the feasibility of using GNS as contrast agents to visualize tumors using NBI.

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“…Absorption of light in tissue leads to a small increase in temperature, which results in volume expansion and generation of pressure waves. [1][2][3][4][5] Nanoparticulate contrast agents for molecular imaging are of particular interest for diagnostic imaging in medicine because of their strong signal per molecular recognition site. For PA imaging, contrast materials showing plasmon resonance or NIRabsorbing properties are of great interest.…”

mentioning

confidence: 99%

Preparation and biological evaluation of multifunctional PLGA-nanoparticles designed for photoacoustic imaging

Kohl

Kaiser²,

Bost

et al. 2011

Nanomedicine: Nanotechnology, Biology and Medicine

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P6A-5 Real-Time Optoacoustic Imaging Using Near Infrared Absorbing Gold Nanoshells For Contrast Enhancement

Cited by 5 publications

References 4 publications

Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

Near-infrared narrow-band imaging of gold/silica nanoshells in tumors

Preparation and biological evaluation of multifunctional PLGA-nanoparticles designed for photoacoustic imaging

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