Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

Abstract

We demonstrate the capability of using immunotargeted gold nanoshells as contrast agents for in vitro two-photon microscopy. The two-photon luminescence properties of different-sized gold nanoshells are first validated using near-infrared excitation at 780 nm. The utility of two-photon microscopy as a tool for imaging live HER2-overexpressing breast cancer cells labeled with anti-HER2-conjugated nanoshells is then explored and imaging results are compared to normal breast cells. Five different imaging channels are simultaneously examined within the emission wavelength range of 451-644 nm. Our results indicate that under near-infrared excitation, superior contrast of SK-BR-3 cancer cells labeled with immunotargeted nanoshells occurs at an emission wavelength ranging from 590 to 644 nm. Luminescence from labeled normal breast cells and autofluorescence from unlabeled cancer and normal cells remain imperceptible under the same conditions.

Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia

“…Their potential use in the field ranges from detection by imaging, labeling, or sensing [1][2][3][4][5] to treatment, including drug delivery and phototherapy [6][7][8][9][10][11][12][13][14].…”

Microspectroscopic characterisation of gold nanorods for cancer detection

“…154,163,[166][167][168][169]179,209,210 This is similar to the spectral emission properties excited with weak fields in linear fluorescence spectroscopy (Table 5.1) and suggests that the emission is characteristic of the gold metal.…”

Anisotropic Gold-based Nanoparticles: Preparation, Properties, and Applications

“…87 Imura et al demonstrated the scanning of near-field optical microscopic images of two-photon luminescence from gold nanorods. 88 Cellular uptake of spherical gold nanoparticles, 89 nanorods, 90,91 nanoshells, 92 and nanocages 93 was observed using a two-photon luminescence microscope. Although femtosecond pulsed laser light is required to observe it, this technique can be expanded to in vivo direct imaging of a single gold nanorod.…”