p63 Expression and its Relation to Epithelial Cells Proliferation in Dentigerous Cyst, Odontogenic Keratocyst, and Ameloblastoma

Alsaegh, Mohammed Amjed; Altaie, Alaa Muayad; Zhu, Shu

doi:10.1007/s12253-019-00680-7

Cited by 10 publications

(11 citation statements)

References 35 publications

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“…The regulation of expression of maspin, however, is a complex process, influenced by P53 and other members of the family, namely, P63 and P73 [ 51 – 53 ]. Studies have shown different patterns of expression of the P53 family proteins in the OKC compared to other odontogenic cysts [ 8 , 12 , 14 , 15 , 17 – 25 ]. This finding warrants additional research on maspin expression in the OKC concerning the P53 gene family members.…”

Section: Discussionmentioning

confidence: 99%

“…Various immunohistochemical studies have been conducted to investigate the biologic nature of OKCs, such as those assessing proliferation markers (Ki-67 and PCNA) [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and the tumor suppressor gene P53 and other members of the P53 family [8,12,14,15,17,18,[20][21][22][23][24][25]. Higher proliferative activity and more significant or different P53 expression in the OKC compared to other odontogenic cysts have been reported [5-9, 12-14, 16-22, 24].…”

Section: Introductionmentioning

confidence: 99%

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Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Hammad

Nagrash

Safadi

2020

International Journal of Dentistry

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The odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name “keratocystic odontogenic tumor” in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of an important regulatory protein (maspin), an important integral membrane proteoglycan (syndecan-1), and a universal proliferation marker (Ki-67) in the epithelium of the OKC were investigated in comparison with the dentigerous cyst (DC) and ameloblastoma (AB). Twenty-six OKCs, eleven DCs, and ten conventional ABs were immunohistochemically stained for maspin, syndecan-1, and Ki-67. ImageJ was used to analyze the positivity of maspin and syndecan-1. The Ki-67 score was calculated as the percentage of positive nuclei in 5 high power fields. Analysis of variance (ANOVA) test and Student t-test were used as appropriate. Lower expressions of maspin were noted in OKC and DC compared to those in AB, and lower expressions of syndecan-1 were noted in OKC and AB compared to those in DC. The differences, however, did not reach statistical significance (ANOVA and t-test: P>0.05). The Ki-67 score was significantly higher in OKC than in DC (t-test: P<0.05), and not significantly different from AB (t-test: P>0.05). In conclusion, expressions of maspin and syndecan-1 are not strongly representative of differences in behavior between OKC, AB, and DC. However, the expression of Ki-67 indicates comparable proliferative activities of OKC and AB, which are higher than that of DC. Further investigation on the biologic behavior of OKC is still recommended to arrive at more specific conclusions regarding its classification.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Hammad

Nagrash

Safadi

2020

International Journal of Dentistry

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“…40 In addition, p63 plays a crucial role in the proliferation of normal odontogenic epithelial cells. 41 In malignant lesions with a significant clear cell component, including salivary gland MEC, CCOC, and metastatic clear cell carcinoma, the expression of CKs is variable. The study of Azevedo et al 42 showed that the CK immunoprofile in MEC in the different histological grades of malignancy was changeable and reproduced the encountered cellular types, but in general all CKs were positive.…”

Section: Discussionmentioning

confidence: 99%

A Rare Case of an Aggressive Clear Cell Variant of Calcifying Epithelial Odontogenic Tumor in the Posterior Maxilla

et al. 2020

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A clear cell variant of calcifying epithelial odontogenic tumor is a rare benign odontogenic neoplasm, accounting for 33 cases described in the literature. In this article, we report a challenging example of clear cell variant of calcifying epithelial odontogenic tumor of the posterior maxilla in a 45-year-old female patient showing locally aggressive growth and recurrence. Microscopically, islands of polyhedral cells containing abundant cytoplasm, well-developed intercellular bridges blended with clear cells were observed. The nuclei were frequently pleomorphic and permeated by hyaline calcified material. Immunohistochemistry revealed positivity for pan-cytokeratin (AE1/AE3), cytokeratins (CK-14 and CK-19), Bcl-2, p53, and p63. The Ki-67 proliferative index was ~10%. As odontogenic tumors are rare, when a significant clear cell component is observed, the differential diagnosis with other lesions of the jaws with similar morphology, including other odontogenic tumors with prominent clear cell component, clear cell odontogenic carcinomas, and metastatic tumors, is difficult.

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“…To date, most molecular studies on AB have focused on exploring markers and genetic variation, which help to ensure diagnosis and better predict their prognosis [7][8][9]. Increasing evidence demonstrates that abnormal expression of multiple genes contributes to the development and progression of AB [10][11][12]. For example, a recent study found that FOXP1/SOX10 can distinguish AB from basaloid salivary gland tumors [13].…”

Section: Introductionmentioning

confidence: 99%

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

et al. 2020

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Background:Ameloblastoma (AB) is a common odontogenic epithelial tumor, with locally invasive behavior and high recurrence. In this study, we hypothesized that miR-524-5p could be involved in the tumor microenvironment by targeting interleukin-33 (IL-33)/suppression of tumorigenicity 2 (ST2) in AB. Material/Methods:The microRNA (miRNA) expression profile of AB tissues and normal oral mucosa tissues (NOM; 6 paired samples) was analyzed. The miRNAs with fold change ³2 and P<0.05 were considered to be differentially expressed. Among them, downregulated miR-524-5p was verified by real-time qPCR. Potential targets of miR-524-5p were predicted by bioinformatics analysis. The expression levels of target genes were detected using real-time qPCR and Western blot, respectively. Immunohistochemistry analysis of target genes was performed, and we also assessed the correlation between miR-524-5p and its target. Results:Microarray analysis results first indicated miR-524-5p is a downregulated miRNA in AB tissues. Real-time qPCR results confirmed the expression pattern of miR-524-5p in AB tissues. Moreover, IL-33 and its receptor ST2 were significantly overexpressed. As shown in immunohistochemistry results, IL-33 was positively expressed in lymphocytes and plasma cells, suggesting that IL-33/ST2 participates in tumor immune responses in the tumor microenvironment. Correlation analysis suggested that miR-524-5p expression was negatively correlated with IL-33/ST2. Conclusions:Our findings reveal that downregulated miR-524-5p can participate in the tumor microenvironment of AB by targeting the IL-33/ST2 axis.

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p63 Expression and its Relation to Epithelial Cells Proliferation in Dentigerous Cyst, Odontogenic Keratocyst, and Ameloblastoma

Cited by 10 publications

References 35 publications

Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

A Rare Case of an Aggressive Clear Cell Variant of Calcifying Epithelial Odontogenic Tumor in the Posterior Maxilla

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

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