Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Abstract

The odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name “keratocystic odontogenic tumor” in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of an important regulatory protein (maspin), an important integral membrane proteoglycan (syndecan-1), and a universal proliferation marker (Ki-67) in the epithelium of the OKC were investigated in comparison with the dentigerous cyst (DC) and ameloblastoma (AB). Twenty-six OKCs, eleven DCs, and ten conventional ABs were immunohistochemically stained for maspin, syndecan-1, and Ki-67. ImageJ was used to analyze the positivity of maspin and syndecan-1. The Ki-67 score was calculated as the percentage of positive nuclei in 5 high power fields. Analysis of variance (ANOVA) test and Student t-test were used as appropriate. Lower expressions of maspin were noted in OKC and DC compared to those in AB, and lower expressions of syndecan-1 were noted in OKC and AB compared to those in DC. The differences, however, did not reach statistical significance (ANOVA and t-test: P>0.05). The Ki-67 score was significantly higher in OKC than in DC (t-test: P<0.05), and not significantly different from AB (t-test: P>0.05). In conclusion, expressions of maspin and syndecan-1 are not strongly representative of differences in behavior between OKC, AB, and DC. However, the expression of Ki-67 indicates comparable proliferative activities of OKC and AB, which are higher than that of DC. Further investigation on the biologic behavior of OKC is still recommended to arrive at more specific conclusions regarding its classification.

The expression of BMI-1 in ameloblastoma and dentigerous cyst

“…The same distribution was described in previous studies employing ki-67 which is a known proliferation marker reported to be present during all active phases of the cell cycle and absent in the quiescent cells. [14][15][16] The currently observed localization stands in a line with previous studies suggesting that the peripheral cells might have an important role in the proliferative activity of Am neoplasm. Further support to this notion is provided by studies that reported that mutated p53; a tumor suppressor gene was mostly located in the peripheral columnar cells.…”

“…The current result agrees with other studies employing the ki-67 which showed weak reactivity in the basal and suprabasal cells of the epithelial lining of DC. 16,20 This reflects the low activity in the proliferative component of the lining and consequently the known indolent course of DC. 3,5,16,21,22 An original finding in our research is the dual staining pattern, nuclear and cytoplasmic, with a predominance of the former pattern in the Am group of neoplastic nature.…”

“…16,20 This reflects the low activity in the proliferative component of the lining and consequently the known indolent course of DC. 3,5,16,21,22 An original finding in our research is the dual staining pattern, nuclear and cytoplasmic, with a predominance of the former pattern in the Am group of neoplastic nature. The cytoplasmic immunoreaction in the current study is justified by the study which proved the presence of BMI-1 in the inner mitochondrial membrane.…”

Immunohistochemical Expression of CK14 and Bcl-2 in Odontogenic Keratocyst and Its Variants

“…Odontogenic cysts belong to a heterogeneous group of lesions that constitute innocuous inflammatory cysts to develop cysts, sometimes behave in a locally aggressive manner, and may show variegated histologic morphologies 10 . The OKCs are aggressive cystic jaw lesions with an increased propensity for recurrence and have a high growth potential 1 . Marsupialization is a treatment of choice used by surgeons to reduce the volume of OKCs before enucleation and minimize vascular and neurological complications, unesthetic outcomes, and morbidity.…”

Imunoexpressão do marcador de células-tronco tumorais ALDH1 e do antígeno de proliferação Ki67 em lesões odontogênicas

“…O nível de expressão aumenta na fase G1 para a mitose e, em seguida, diminui ou desaparece logo após a mitose. Tais características o tornam um excelente marcador para determinar a chamada fração de crescimento e status de proliferação de uma determinada população(83)(84)(85)(86)(87)(88)(89). por exemplo.…”