p53–RNA interactions: New clues in an old mystery

Riley, Kasandra; Maher, L. James

doi:10.1261/rna.673407

Cited by 60 publications

(61 citation statements)

References 82 publications

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“…Proteins that can bind both DNA and RNA are ideal candidates for transcriptional and/or epigenetic regulators, in an RNAguiding manner. Like the multifunction protein EZH2, there are some well-studied DNA-binding proteins, such as DNA methylase [108], Smad [109], p53 [110,111], and others [112], that bind RNAs. Similarly, some RBPs can also bind DNA [113115].…”

Section: Studying Epigenetic Modification-associated Rnasmentioning

confidence: 99%

CLIP: viewing the RNA world from an RNA-protein interactome perspective

Zhang

Xie²,

Xu³

et al. 2015

Sci. China Life Sci.

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The pervasive transcription of the genome creates many types of non-coding RNAs (ncRNAs). However, we know very little regarding the functions and the regulatory mechanisms of these ncRNAs. Exploring the interactions of RNA and RNA binding proteins (RBPs) is vital because it can allow us to truly understand how these ncRNAs behave in vivo. High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP or CLIP-seq) and its variants have been successfully used as systemic techniques to study RBP binding sites. In this review, we will explain the major differences between the CLIP techniques, summarize successful applications of these techniques, discuss limitations of CLIP, present some suggested solutions and project their promising future roles in studying the RNA world. CLIP, CLASH, RPBs, ncRNAs, functional RNomics Citation:Zhang

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Section: Studying Epigenetic Modification-associated Rnasmentioning

confidence: 99%

CLIP: viewing the RNA world from an RNA-protein interactome perspective

Zhang

Xie²,

Xu³

et al. 2015

Sci. China Life Sci.

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“…Together with other recent results [6,7], this work illuminates a historically mysterious aspect of p53 biology. The physiological significance of RNA binding by p53 remains obscure [4]. …”

Section: Rna Co-immunoprecipitation After Clipmentioning

confidence: 99%

“…A peculiar observation is RNA binding by the C-terminus of p53 ( Fig. 1A; [4]). Although many RNAbinding proteins are characterized by RNA-recognition motifs (RRMs; [5]), the interaction between p53 and RNA involves the C-terminal 30 amino acids of p53 [6,7], which do not form a RRM but are disordered in solution [7,8] and do not reorganize upon RNA binding [7,9].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, Galy et al reported that recombinant p53 can bind the 5'-UTR of the FGF-2 mRNA [18]. In contrast, reports of p53 RNA-RNA annealing activity [13] and sequence-nonspecific RNA binding [4,6,7,19] suggested that p53 binds RNA promiscuously. Acetylation of four p53 C-terminal lysine residues eliminates detectable RNA binding in vitro [7], as it does for sequence-nonspecific DNA binding [8].…”

Section: Introductionmentioning

confidence: 99%

“…Our previous studies of RNA binding by p53 in yeast and in vitro demonstrated that p53 with incomplete post-translational modifications binds RNA without apparent sequence-or structure-specificity [4,6,7]. It is possible that a partially-modified form of p53 could exist in vivo with one or more specific RNA partners.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of p53–RNA interactions in cultured human cells

Riley

Maher

2007

Biochemical and Biophysical Research Communications

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Tumor suppressor p53 is a well-characterized transcription factor that binds DNA. More enigmatic are the RNA-binding properties of p53 and their physiological relevance. We used three sensitive co-immunoprecipitation methods in an attempt to detect RNAs that tightly associate with p53 in cultured human cells. Although recombinant p53 protein binds RNA in a sequence-nonspecific mode, we do not detect specific in vivo RNA binding by p53. These results suggest that RNA binding is prevented by post-translational p53 modifications. A ribonucleoprotein (not p53) is purified by multiple IgG monoclonal antibodies (including anti-p53 antibodies) from both p53 +/+ and p53 null cells. Caution is therefore required in interpreting RNA co-immunoprecipitation experiments. Though not formally excluded, these results do not support models in which p53 binds specific RNA partners in vivo.

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P53 long noncoding RNA regulatory network in cancer development

Aravindhan

Younus

Lafta

et al. 2021

Cell Biology International

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The protein p53 as a transcription factor with strong tumor-suppressive activities is known to trigger apoptosis via multiple pathways and is directly involved in the recognition of DNA damage and DNA repair processes. P53 alteration is now recognized as a common event in the pathogenesis of many types of human malignancies. Deregulation of tumor suppressor p53 pathways plays an important role in the activation of cell proliferation or inactivation of apoptotic cell death during carcinogenesis and tumor progression. Mounting evidence indicates that the p53 status of tumors and also the regulatory functions of p53 may be relevant to the long noncoding RNAs (lncRNA)-dependent gene regulation programs. Besides

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p53–RNA interactions: New clues in an old mystery

Cited by 60 publications

References 82 publications

CLIP: viewing the RNA world from an RNA-protein interactome perspective

CLIP: viewing the RNA world from an RNA-protein interactome perspective

Analysis of p53–RNA interactions in cultured human cells

P53 long noncoding RNA regulatory network in cancer development

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