p53 and p21<sup>WAF1/CIP1</sup>overexpression at the invasive front of lower lip squamous cell carcinoma

Horta, Martinho Campolina Rebello; Assis, Luis Alpino Prandini De; Souza, Antônio Francisco de; Araújo, Vera Cavalcanti de; Gomez, Ricardo Santiago; Aguiar, Maria Cássia Ferreira de

doi:10.1111/j.1600-0714.2007.00495.x

Cited by 21 publications

(20 citation statements)

References 42 publications

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“…Activation of NF-κB in MNNG-induced gastric tumours was associated with upregulation of cyclins and PCNA with simultaneous downregulation of p53 and p21, consistent with similar observations in other malignant tumours [12,[30][31][32]. Our results also demonstrate the ability of eugenol to fine tune the expression of NF-κB target genes and halt cell cycle progression.…”

Section: Discussionsupporting

confidence: 91%

Eugenol inhibits cell proliferation via NF-κB suppression in a rat model of gastric carcinogenesis induced by MNNG

et al. 2009

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The modulation of intracellular nuclear factor-kappaB (NF-κB) signaling pathway involved in the deregulated expression of cell proliferation and cell cycle regulatory molecules is a pragmatic approach for chemoprevention. Eugenol (4-allyl-1-hydroxy-2-methoxybenzene), a natural phenolic constituent of oils of cloves is known to possess attractive remedial features. In the present study, we investigated the modulatory effects of eugenol on NF-κB signaling in a rat model of gastric carcinogenesis induced by N-methyl-N(')-nitro-N-nitrosoguanidine (MNNG) by analysing the expression of nuclear factor-kappaB (NF-κB) family members ((NF-κB (p50 and p65), inhibitor of kappaB alpha (IκBα), phosphorylated IκBα (p-IκBα), IκB kinase β (IKKβ)) and the NF-κB target genes that promote (e.g., cyclin D1, cyclin B and PCNA) or inhibit (e.g., p53, p21, and Gadd45) cell proliferation and cell survival. MNNG-induced gastric tumours were characterized by NF-κB activation that correlated with upregulation of IKKβ, and phosphorylation and degradation of IκBα. Furthermore, upregulation of cyclins and PCNA with downregulation of p21, p53, and Gadd45 suggested that the proliferative advantage in gastric carcinomas is dependent on elevated constitutive NF-κB activity. Administration of eugenol significantly reduced the incidence of MNNG-induced gastric tumours by suppressing NF-κB activation and modulating the expression of NF-κB target genes that regulate cell proliferation and cell survival. The targeting of NF-κB signaling pathway by eugenol may have a significant impact on chemopreventive and therapeutic approaches for cancer.

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Section: Discussionsupporting

confidence: 91%

Eugenol inhibits cell proliferation via NF-κB suppression in a rat model of gastric carcinogenesis induced by MNNG

et al. 2009

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“…Therefore, our data showed that immunoreactivity for p53 protein was observed in both premalignant and malignant lesions of the lower lip. These data along with previous studies [13,23] corroborate and emphasize that the overexpression of p53 protein is mainly present in the early stages of oral carcinogenesis process.…”

Section: Discussionsupporting

confidence: 79%

Correlation between cell cycle proteins and hMSH2 in actinic cheilitis and lip cancer

et al. 2016

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This study aims to evaluate and verify the relationship between the immunoexpression of hMSH2, p53 and p21 in actinic cheilitis (AC) and lower lip squamous cell carcinoma (SCC) cases. Forty AC and 40 SCC cases were submitted to immunoperoxidase method and quantitatively analyzed. Expression was compared by Mann-Whitney test, Student t test or one-way ANOVA. To correlate the variables, Pearson's correlation coefficient was calculated. The expression of p53 and p21 showed no significant differences between histopathological grades of AC or lower lip SCC (p [ 0.05). Immunoexpression of p53 was higher in SCC than in AC (p \ 0.001), while p21 expression was more observed in AC when compared to SCC group (p = 0.006). The AC group revealed an inverse correlation between p53 and hMSH2 expression (r = -0.30, p = 0.006). Alterations in p53 and p21 expression suggest that these proteins are involved in lower lip carcinogenesis. Moreover, p53 and hMSH2 seem to be interrelated in early events of this process.

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“…Em um estudo conduzido por Horta et al (21) , foi avaliada uma série de 21 carcinomas de lábio, obtendo-se expressão positiva da proteína p53, verificada pelo clone DO-1 em 85,7% dos casos avaliados. Em uma análise de 79 carcinomas de lábio, Garcia-Montesinos-Perea et al (16) verificaram imunopositividade para a proteína p53 por meio do clone DO-7 em 70,6% dos casos.…”

Section: Discussionunclassified

Análise comparativa da imunoexpressão da proteína p53 (clones DO-7 e PAb-240) em carcinomas de células escamosas intrabucais e labiais

Júnior¹,

Bernardo²,

Balassiano³

et al. 2009

J. Bras. Patol. Med. Lab.

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Introdução: A carcinogênese caracteriza-se como um processo multifatorial, e a inativação da proteína p53 é uma alteração genética comumente observada nos carcinomas de células escamosas de boca (CCEB). Objetivo: Analisar e comparar a imunoexpressão da proteína p53, por meio dos clones DO-7 e PAb-240, em CCEB com localização intrabucal e em lábio inferior. Material e métodos: Foram selecionados 40 casos de CCEB, sendo 20 de localização intrabucal e 20 em lábio inferior. Foi realizado um estudo imuno-histoquímico utilizando os anticorpos anti-p53 clone DO-7 e PAb-240. A imunoquantificação foi realizada por meio de análise digital de imagem, e os resultados, submetidos a tratamentos estatísticos. Resultados: A imunoexpressão da proteína p53 foi verificada com o anticorpo DO-7 em 13 casos (65%) de carcinoma intrabucal e em 19 (95%) de carcinoma de lábio inferior. Imunorreatividade para o anticorpo PAb-240 foi observada em 9 casos (45%) de lesões intrabucais e em 15 (75%) localizados em lábio inferior. Não foram observadas, segundo o teste de Mann-Whitney, diferenças estatisticamente significativas (p > 0,05) na expressão da proteína p53 entre as duas localizações estudadas, independentemente do anticorpo avaliado. Foram identificadas, pelo teste de Wilcoxon, diferenças estatisticamente significativas entre a expressão dos clones DO-7 e PAb-240 em cada um dos grupos analisados (valor p = 0,013 -lábio inferior; valor p = 0,016 -intrabucal). Conclusões: A expressão da proteína p53 observada nos CCEB, com localizações intrabucais e labiais, sugere a ocorrência de mutações no gene TP53. As diferenças quantitativas obtidas entre os anticorpos estudados, independentemente da localização das lesões, refletem uma especificidade distinta entre os clones DO-7 e PAb-240. O desenvolvimento de mais estudos será fundamental para estabelecer o anticorpo mais adequado para proteína p53 em CCEB. Background: Carcinogenesis is a multifactorial process and inactivation of p53 protein is a genetic change commonly observed in oral squamous cell carcinomas (OSCC). Objectives: To analyze and compare the expression of p53 protein through antibodies DO-7 and PAb-240 in OSCC samples located in the oral cavity and lower lip. Material and methods: Forty cases of OSCC were selected and divided into oral cavity and lower lip groups (20 cases each).Immunohistochemical technique was performed using antibodies

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p53 and p21^WAF1/CIP1overexpression at the invasive front of lower lip squamous cell carcinoma

Abstract: Our results indicate that p53 and p21(WAF1/CIP1) overexpression is important in LLSCC pathogenesis, reinforce that IF is the most important area for tumor behavior, and support that p53-independent mechanisms should be involved in the expression of p21(WAF1/CIP1).

Cited by 21 publications

References 42 publications

Eugenol inhibits cell proliferation via NF-κB suppression in a rat model of gastric carcinogenesis induced by MNNG

Eugenol inhibits cell proliferation via NF-κB suppression in a rat model of gastric carcinogenesis induced by MNNG

Correlation between cell cycle proteins and hMSH2 in actinic cheilitis and lip cancer

Análise comparativa da imunoexpressão da proteína p53 (clones DO-7 e PAb-240) em carcinomas de células escamosas intrabucais e labiais

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p53 and p21WAF1/CIP1overexpression at the invasive front of lower lip squamous cell carcinoma

Abstract: Our results indicate that p53 and p21(WAF1/CIP1) overexpression is important in LLSCC pathogenesis, reinforce that IF is the most important area for tumor behavior, and support that p53-independent mechanisms should be involved in the expression of p21(WAF1/CIP1).

Cited by 21 publications

References 42 publications

Eugenol inhibits cell proliferation via NF-κB suppression in a rat model of gastric carcinogenesis induced by MNNG

Eugenol inhibits cell proliferation via NF-κB suppression in a rat model of gastric carcinogenesis induced by MNNG

Correlation between cell cycle proteins and hMSH2 in actinic cheilitis and lip cancer

Análise comparativa da imunoexpressão da proteína p53 (clones DO-7 e PAb-240) em carcinomas de células escamosas intrabucais e labiais

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p53 and p21^WAF1/CIP1overexpression at the invasive front of lower lip squamous cell carcinoma