P450-mediated detoxification of botanicals in insects

Cui, Shishuang; Wang, Lei; Ma, Long; Geng, Xueqing

doi:10.1007/s12600-016-0550-1

Cited by 26 publications

(26 citation statements)

References 102 publications

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“…RT-qPCR data confirmed differential expression of CYP3A56 in Varroa after FA exposure. In vertebrates, the CYP3A subfamily of the cytochrome P450 superfamily plays a dominant role in metabolic clearance of numerous substances including toxins, pesticides and therapeutic drugs 85 , 86 . Most studies on CYP3A have been conducted in vertebrates, while little is known about non-vertebrate species, especially varroa mites.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomics indicates endogenous differences in detoxification capacity after formic acid treatment between honey bees and varroa mites

Genath

Sharbati

Buer

et al. 2020

Sci Rep

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Formic acid (FA) has been used for decades to control Varroa destructor, one of the most important parasites of the western honey bee, Apis mellifera. The rather unselective molecular mode of action of FA and its possible effects on honeybees have long been a concern of beekeepers, as it has undesirable side effects that affect the health of bee colonies. This study focuses on short-term transcriptomic changes as analysed by RNAseq in both larval and adult honey bees and in mites after FA treatment under applied conditions. Our study aims to identify those genes in honey bees and varroa mites differentially expressed upon a typical FA hive exposure scenario. Five detoxification-related genes were identified with significantly enhanced and one gene with significantly decreased expression under FA exposure. Regulated genes in our test setting included members of various cytochrome P450 subfamilies, a flavin-dependent monooxygenase and a cytosolic 10-formyltetrahydrofolate dehydrogenase (FDH), known to be involved in formate metabolism in mammals. We were able to detect differences in the regulation of detoxification-associated genes between mites and honey bees as well as between the two different developmental stages of the honey bee. Additionally, we detected repressed regulation of Varroa genes involved in cellular respiration, suggesting mitochondrial dysfunction and supporting the current view on the mode of action of FA—inhibition of oxidative phosphorylation. This study shows distinct cellular effects induced by FA on the global transcriptome of both host and parasite in comparison. Our expression data might help to identify possible differences in the affected metabolic pathways and thus make a first contribution to elucidate the mode of detoxification of FA.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptomics indicates endogenous differences in detoxification capacity after formic acid treatment between honey bees and varroa mites

Genath

Sharbati

Buer

et al. 2020

Sci Rep

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“…Many insect CYPs also play roles in detoxification of xenobiotics. Insect CYPs are inducible in response to botanical insecticides and/or plant secondary metabolites [ 11 , 142 , 143 ]. Also, some plant CYPs involved in the detoxification of xenobiotics such as herbicides [ 9 ].…”

Section: Xenobiotic-metabolizing Cypsmentioning

confidence: 99%

“…In insects, CYPs are involved in the production of defence toxins and pheromones [ 9 , 10 ]. CYP-mediated detoxification of plant compounds, as well as insecticides, has also been reported [ 11 ]. Plant genomes tend to contain many more CYP genes than animal genomes, e.g., 334 in Oryza sativa (rice), 245 in Arabidopsis thaliana (thale cress), and 318 in Zea mays (corn), possibly because plants produce a wide variety of secondary metabolites [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Fungal Cytochrome P450s and the P450 Complement (CYPome) of Fusarium graminearum

Shin¹,

Kim²,

Lee³

et al. 2018

Toxins

112

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Cytochrome P450s (CYPs), heme-containing monooxygenases, play important roles in a wide variety of metabolic processes important for development as well as biotic/trophic interactions in most living organisms. Functions of some CYP enzymes are similar across organisms, but some are organism-specific; they are involved in the biosynthesis of structural components, signaling networks, secondary metabolisms, and xenobiotic/drug detoxification. Fungi possess more diverse CYP families than plants, animals, or bacteria. Various fungal CYPs are involved in not only ergosterol synthesis and virulence but also in the production of a wide array of secondary metabolites, which exert toxic effects on humans and other animals. Although few studies have investigated the functions of fungal CYPs, a recent systematic functional analysis of CYP genes in the plant pathogen Fusarium graminearum identified several novel CYPs specifically involved in virulence, asexual and sexual development, and degradation of xenobiotics. This review provides fundamental information on fungal CYPs and a new platform for further metabolomic and biochemical studies of CYPs in toxigenic fungi.

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“…Our results are close to the research of Rupasinghe [ 58 ], in which a comparison of the homology models for CYP688 and CYP321A1 described their substrate-binding cavities, which were predicted to be more spacious in these two enzymes that metabolize a wider range of compounds. Therefore, we hypothesized that CYP6CS3 can present a greater metabolic ability than CYP408A3 [ 59 , 60 ]. This result also confirmed the phenomenon that dsCYP6CS3 had a high interference efficiency and a high degree of P450 enzyme activity decline mediated by CYP6CS3 .…”

Section: Discussionmentioning

confidence: 99%

Cloning and Functional Verification of CYP408A3 and CYP6CS3 Related to Chlorpyrifos Resistance in the Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

Ruan

Liu

Gong

et al. 2021

Biology

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The white-back planthopper (WBPH), Sogatella furcifera, mainly harms rice and occurs in most rice regions in China and Asia. With the use of chemical pesticides, S. furcifera has developed varying degrees of resistance to a variety of pesticides. In our study, a chlorpyrifos-resistant population (44.25-fold) was built through six generations of screening with a sublethal dose of chlorpyrifos (LD50) from a field population. The expression levels of ten selected resistance-related P450 genes were analyzed by RT-qPCR and found that CYP408A3 and CYP6CS3 were significantly more expressed in the third instar nymphs of the XY17-G5 and XY17-G6 populations, about 25-fold more than the Sus-Lab strain, respectively (p < 0.01). To elucidate their molecular function in the development of resistance towards chlorpyrifos, we cloned two P450 full lengths and predicted their tertiary protein structures. CYP408A3 and CYP6CS3 were also downregulated after injecting dsCYP408A3, dsCYP6CS3, or their mixture compared to the control group. Moreover, the mortality rates of the dsCYP6CS3 (91.7%) and the mixture injection treatment (93.3%) treated by the LC50 concentration of chlorpyrifos were significantly higher than the blank control group (51.7%) and dsCYP408A3 injection treatment (69.3%) at 72 h (p < 0.01). Meanwhile, the P450 enzyme activities in the dsRNA treatments were lower than that in the control (XY17-G6) (p < 0.01). Therefore, the P450 gene CYP6CS3 may be one of the main genes in the development of chlorpyrifos resistance in S. furcifera.

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P450-mediated detoxification of botanicals in insects

Cited by 26 publications

References 102 publications

Comparative transcriptomics indicates endogenous differences in detoxification capacity after formic acid treatment between honey bees and varroa mites

Comparative transcriptomics indicates endogenous differences in detoxification capacity after formic acid treatment between honey bees and varroa mites

Fungal Cytochrome P450s and the P450 Complement (CYPome) of Fusarium graminearum

Cloning and Functional Verification of CYP408A3 and CYP6CS3 Related to Chlorpyrifos Resistance in the Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

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