Fungal Cytochrome P450s and the P450 Complement (CYPome) of Fusarium graminearum

Shin, Jiyoung; Kim, Jung-Eun; Lee, Yin-Won; Son, Hokyoung

doi:10.3390/toxins10030112

Cited by 112 publications

(74 citation statements)

References 178 publications

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“…This indicates that P450s play a key role in fungal species’ physiology in terms of their primary and secondary metabolism. Fungal P450s’ role in fungal species’ physiology and their biotechnological application has been thoroughly reviewed [ 8 , 9 , 10 , 11 ]. Among all fungal P450 applications, the use of P450s as drug targets against fungal pathogens is well explored [ 12 , 13 ] because of their binding affinity to azole drugs, which are widely used for treating fungal infections [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Blooming of Unusual Cytochrome P450s by Tandem Duplication in the Pathogenic Fungus Conidiobolus coronatus

Ngwenya

Chen

Basson

et al. 2018

IJMS

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While the Zygomycete fungus Conidiobolus coronatus primarily infects insects, it can be pathogenic to mammals as well, including humans. High variability in the treatment of this fungal infection with currently available drugs, including azole drugs is a very common phenomenon. Azoles bind to the cytochrome P450 monooxygenases (P450s/CYP) including CYP51, a sterol 14-α-demethylase, inhibiting the synthesis of cell membrane ergosterol and thus leading to the elimination of infecting fungi. Despite P450’s role as a drug target, to date, no information on C. coronatus P450s has been reported. Genome-wide data mining has revealed the presence of 142 P450s grouped into 12 families and 21 subfamilies in C. coronatus. Except for CYP51, the remaining 11 P450 families are new (CYP5854-CYP5864). Despite having a large number of P450s among entomopathogenic fungi, C. coronatus has the lowest number of P450 families, which suggests blooming P450s. Further analysis has revealed that 79% of the same family P450s is tandemly positioned, suggesting that P450 tandem duplication led to the blooming of P450s. The results of this study; i.e., unravelling the C. coronatus P450 content, will certainly help in designing experiments to understand P450s’ role in C. coronatus physiology, including a highly variable response to azole drugs with respect to P450s.

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Section: Introductionmentioning

confidence: 99%

Blooming of Unusual Cytochrome P450s by Tandem Duplication in the Pathogenic Fungus Conidiobolus coronatus

Ngwenya

Chen

Basson

et al. 2018

IJMS

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“…The coexpression cluster also contains a variety of transporters such as Major Facilitator Superfamily (MFS) transporter that could be used to secrete gliotoxin and other metabolites potentially synthesized by the coexpression cluster. Additionally, the coexpression cluster contains large numbers of cytochrome P450s, which are decorative enzymes essential for secondary metabolite synthesis (42). In addition to the discussed genes, we observed multiple genes coding for hypothetical proteins in the cluster.…”

Section: Figure 1 Coexpression Analysis Of Specialized Metabolic Hubmentioning

confidence: 81%

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

Lim

Koh

Moo

et al. 2020

Preprint

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ABSTRACTThe fungi kingdom is composed of eukaryotic heterotrophs, which are responsible for balancing the ecosystem and play a major role as decomposers. They also produce a vast diversity of secondary metabolites, which have antibiotic or pharmacological properties. However, our lack of knowledge of gene function in fungi precludes us from tailoring them to our needs and tapping into their metabolic diversity. To remedy this, we gathered genomic and gene expression data of 19 most widely-researched fungi to build a database, fungi.guru, which contains tools for cross-species identification of conserved pathways, functional gene modules, and gene families. We exemplify how our database can elucidate the molecular function, biological process and cellular component of genes involved in various biological processes, by identifying a secondary metabolite pathway producing gliotoxin in Aspergillus fumigatus, the catabolic pathway of cellulose in Coprinopsis cinerea and the conserved DNA replication pathway in Fusarium graminearum and Pyricularia oryzae. The database is available at www.fungi.guru.

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“…It has been reported that a more divergent cytochrome P450 oxidase could be involved in secondary biosynthesis [56]. Therefore, we searched the genome of F. filiformis for proteins with a P450 conserved domain using the NCBI CDD tool and BLASTp [40].…”

Section: Cytochrome P450s In the F Filiformis Genomementioning

confidence: 99%

Genome-wide analysis and prediction of genes involved in the biosynthesis of polysaccharides and bioactive secondary metabolites in high-temperature-tolerant of wild Flammulina filiformis

Chen

Tang

et al. 2019

Preprint

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Background: Flammulina filiformis (=Asian “F.velutipes”) is a popular commercial edible mushroom. Many bioactive compounds with medicinal effects, such as polysaccharides and sesquiterpenoids, have been isolated and identified from F. filiformis, but their biosynthesis and regulation at the molecular level remains unclear. In this study, we sequenced the genome of the wild strain F. filiformis Liu355, predicated its the biosynthetic gene clusters (BGCs) and profiled the expression of these genes in wild and cultivar strains and in different developmental stages of the wild F. filiformis strain by a comparative transcriptomic analysis. Results: We found that the genome of the F. filiformis was 35.01 M bp in length and harbored 10396 gene models. Thirteen putative terpenoid gene clusters were predicted and 12 sesquiterpene synthase genes belonging to four different groups and two type I polyketide synthase gene clusters were identified in the F. filiformis genome. The number of genes related to terpenoid biosynthesis was higher in the wild strain (119 genes) than in the cultivar strain (81 genes). Most terpenoid biosynthesis genes were upregulated in the primordium and fruiting body of the wild strain, while the polyketide synthase genes were generally upregulated in the mycelium of the wild strain. Moreover, genes encoding UDP-glucose pyrophosphorylase and UDP-glucose dehydrogenase, which are involved in polysaccharide biosynthesis, had relatively high transcript levels both in the mycelium and fruiting body of the wild F. filiformis strain. Conclusions: F. filiformis is enriched in a number of gene clusters involved in the biosynthesis of polysaccharides and terpenoid bioactive compounds and these genes usually display differential expression between wild and cultivar strains, even in different developmental stages. This study expands our knowledge of the biology of F. filiformis and provides valuable data for elucidating the regulation of secondary metabolites in this unique F. filiformis strain.

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Fungal Cytochrome P450s and the P450 Complement (CYPome) of Fusarium graminearum

Cited by 112 publications

References 178 publications

Blooming of Unusual Cytochrome P450s by Tandem Duplication in the Pathogenic Fungus Conidiobolus coronatus

Blooming of Unusual Cytochrome P450s by Tandem Duplication in the Pathogenic Fungus Conidiobolus coronatus

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

Genome-wide analysis and prediction of genes involved in the biosynthesis of polysaccharides and bioactive secondary metabolites in high-temperature-tolerant of wild Flammulina filiformis

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