P4‐033: Microchannel Electrophoresis for Rapid, Low Concentration Detection of Early Amyloid‐beta Aggregation

Hestekin, Christa N.; Kurtz, Jennifer; Lutz-Rechtin, Tammy

doi:10.1016/j.jalz.2014.05.1547

Cited by 5 publications

(3 citation statements)

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“…β-amyloid 42 has been used as a biomarker in CSF for AD. Detection of β-amyloid 42 has been exploited using different analytical techniques, such as mass spectrometry [ 9 ], surface plasmon resonance [ 10 ], scanning tunneling microscopy [ 11 ], electrophoresis [ 12 , 13 ], the enzyme-linked immunosorbent assay (ELISA) [ 14 ], and others. Detection of β-amyloid 42 by the ELISA technique is commonly used in liquid-based test samples.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-Effective Thin Gold Film Biosensor

2017

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A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffered saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The bio-recognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K4Fe(CN)6 and K3Fe(CN)6. Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction. The biosensor was a three-electrode electrochemical system, and the working and counter electrodes were 50 nm thin gold film deposited by a sputtering technique. The reference electrode was a thick-film printed Ag/AgCl electrode. Laser ablation technique was used to define the size and structure of the biosensor. Cost-effective roll-to-roll manufacturing process was employed in the fabrication of the biosensor, making it simple and relatively inexpensive. Self-assembled monolayers (SAM) of 3-Mercaptopropionic acid (MPA) was employed to covalently immobilize the thiol group on the gold working electrode. A carbodiimide conjugation approach using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N–hydroxysuccinimide (NHS) was undertaken for cross-linking antibody of β-amyloid 42 to the carboxylic groups on one end of the MPA. The antibody concentration of β-amyloid 42 used was 18.75 µg/mL. The concentration range of β-amyloid 42 in this study was from 0.0675 µg/mL to 0.5 µg/mL for both PBS and undiluted human serum. DPV measurements showed excellent response in this antigen concentration range. Interference study of this biosensor was carried out in the presence of Tau protein antigen. Excellent specificity of this β-amyloid 42 biosensor was demonstrated without interference from other species, such as T-tau protein.

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Section: Introductionmentioning

confidence: 99%

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-Effective Thin Gold Film Biosensor

2017

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“…Up to date, a few methods including enzyme-linked immunosorbent assay (ELISA) 13 , mass spectrometry 14 , surface plasmon resonance (SPR) 15 , scanning tunneling microscopy (STM) 16 , capillary electrophoresis 17 , spectroscopic ellipsometry 18 , gold nanoparticle-based dot-blot immunoassay 19 , metal semiconductor field effect transistor (MESFET) 20 , microchannel electrophoresis 21 and resonance light scattering 22 have been developed to detect Aβ species. Nevertheless, most of these methods are usually costly, time-consuming, require complicated instruments or lack sensitivity.…”

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confidence: 99%

Non-invasive screening for early Alzheimer’s disease diagnosis by a sensitively immunomagnetic biosensor

Lin

Wei

et al. 2016

Sci Rep

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Amyloid-beta peptide 1–42 (Aβ42) is considered as a reliable biomarker for the early diagnosis of Alzheimer’s disease (AD). Thus, it is urgent to develop a simple and efficient method for the detection of Aβ42. In this work, a reusable biosensor based on magnetic nitrogen-doped graphene (MNG) modified Au electrode for the detection of Aβ42 has been developed. The antibodies of Aβ 1–28 (Aβab) are used as the specific biorecognition element for Aβ42 that were conjugated on the surface of MNG. In the presence of magnetic nanoparticles on MNG, the electrode coating material, the biosensor can be quickly constructed, without requiring an electrode drying process, which reduce the analysis time and is convenient for proceeding to detection. The reusable biosensor with good reproducibility and stability was linear within the range from 5 pg mL−1 to 800 pg mL−1, covering the cut-off level of Aβ42 and a detection limit of 5 pg mL−1 had been achieved. Furthermore, the fabricated biosensor for Aβ42 detection not only improves the detection performance but also reduces the cost and shortens the response time, demonstrating its potential in diagnosing applications.

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“…β-amyloid 42 has been used as a biomarker in CSF for AD. Detection of β-amyloid 42 has been exploited using different analytical techniques, such as mass spectrometry [9], surface plasmon resonance [10], scanning tunneling microscopy [11], electrophoresis [12,13], the enzyme-linked immunosorbent assay (ELISA) [14], and others. Detection of β-amyloid 42 by the ELISA technique is commonly used in liquid-based test samples.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-effective Thin Gold Film Biosensor

Dai¹,

Molazemhosseini²,

Liu³

2017

Preprint

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A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffer saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The biorecognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K 4 Fe (CN) 6 and K 3 Fe (CN) 6 . Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction. The biosensor was a three-electrode electrochemical system, and the working and counter electrodes were 50 nm thin gold film deposited by sputtering technique. The reference electrode was a thick-film printed Ag/AgCl electrode. Laser ablation technique was used to define the size and structure of the biosensor. Cost-effective roll-to-roll manufacturing process was employed in the fabrication of the biosensor making it simple and relatively inexpensive. Self-assembled monolayers (SAM) of 3-Mercaptopropionic acid (MPA) was employed to covalently immobilize the thiol group on the gold working electrode. A carbodiimide conjugation approach using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was undertaken for cross-linking antibody of β-amyloid 42 to the carboxylic groups on one end of the MPA. The antibody concentration of β-amyloid 42 used was 18.75µg/mL. The concentration range of β-amyloid 42 in this study was from 0.0675µg/mL to 0.5µg/mL for both PBS and undiluted human serum. DPV measurements showed excellent response in this antigen concentration range. Interference study of this biosensor was carried out in the presence of Tau protein antigen. Excellent specificity of this β-amyloid 42 biosensor was demonstrated without interference by other species such as T-tau protein.Keywords β-amyloid 42; differential pulse voltammetry; Neuro-degenerative disorders; ferrocyanide/ferricyanide redox couple Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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P4‐033: Microchannel Electrophoresis for Rapid, Low Concentration Detection of Early Amyloid‐beta Aggregation

Cited by 5 publications

References 0 publications

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-Effective Thin Gold Film Biosensor

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-Effective Thin Gold Film Biosensor

Non-invasive screening for early Alzheimer’s disease diagnosis by a sensitively immunomagnetic biosensor

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-effective Thin Gold Film Biosensor

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