P341Hydrogen sulphide treatment of human adipose derived stem cells increases their proliferation, decreases their mitochondrial activity and enhances their antioxidant defenses

Sesamol Enhances Cell Growth and the Biosynthesis and Accumulation of Docosahexaenoic Acid in the Microalga Crypthecodinium cohnii

“…As indicated by Figure B, the intracellular ROS level in C. cohnii supplemented with sesamol was reduced by 63.5% at 24 h of cultivation and remained significantly lower at 48 h, as compared to the sesamol-free cultures. This result is in line with previous works that showed sesamol or other antioxidants have the potential to attenuate intracellular ROS levels, thereby enhancing cell growth. , …”

“…This result is in line with previous works that showed sesamol or other antioxidants have the potential to attenuate intracellular ROS levels, thereby enhancing cell growth. 20,21 To check if the increase in the final cell density is related to the consumption of glucose, the residual glucose in the culture medium supplemented or not with sesamol was monitored, and the results are shown in Figure 2B. During the whole culture period of 96 h, no significant difference in glucose concentration was observed between the sesamol-containing and sesamol-free cultures, suggesting that the presence of sesamol had little effect on the glucose consumption rate by C. cohnii.…”

“…In past decades, efforts have been made to improve the production of DHA by C. cohnii , which can be achieved by enhancing either cell growth or intracellular DHA proportion. , As indicated, DHA production by C. cohnii could be improved by the application of certain elicitors, for example, n -dodecane, acetic acid, and ethanol. − Intracellular DHA content of C. cohnii was improved significantly by the addition of n -dodecane in the medium . Both acetic acid and ethanol enhanced final cell density and DHA production. , It has been reported that antioxidants, such as vitamin C, hydrogen sulfide, ginsenoside, and sesamol, have great potential to enhance the proliferation capacity of a broad range of cells, likely due to their high ability to scavenge intracellular reactive oxygen species (ROS). − Sesamol (3,4-methylenedioxyphenol) is a natural phenolic compound found in sesame oil. In addition to its potent antioxidation activity, sesamol is able to inhibit malic enzyme, which is a major source of NADPH in many oleaginous microorganisms. , …”