P188 Metallo-beta-lactamase producing strains of Pseudomonas aeruginosa in Iran

Nikbin, Vajihe Sadat; Shooraj, Fahimeh; Shafiee, Mohammad; Noveiri, Hanieh

doi:10.1016/s0924-8579(09)70407-8

Cited by 21 publications

(29 citation statements)

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“…by tourists). The most and also the sole frequent reported MBL-producing P. aeruginosa in Iran is blaVIM1 which is previously reported by several researchers (3,16,21,(27)(28)(29) in different cities of Iran, for example, in Ahvaz (south-west of Iran), Tehran (north of Iran), and Arak.…”

Section: Discussionsupporting

confidence: 52%

“…Nonetheless, high-level and broad antimicrobial activity range due to the present of MBLs is not only a major therapeutic problem, but also a major concern in control and management of infection in hospitals and health care centers (10,19,20). Another importance to detect P. aeruginosa carrying MBLs, is because of their role in serious infections like septicemia and pneumonia and resulted to mortality and morbidity in different parts of the world (4,16,21,22). Carbapenems resistances are as a result of outer membrane permeability reduction, efflux pump over expression, alteration of penicillin-binding protein, and presence of carbapenems hydrolyzing enzymes such as β-lactamase (19).…”

Section: Discussionmentioning

confidence: 99%

“…The prepared EDTA-imipenem disk and the imipenem disk itself were placed in a plate containing Muller-Hinton agar with cultured P. aeruginosa. After 16 -18h of incubation at 37°C, an MBL positive organism was approved if the diameter of inhibition zone increased by 7 mm or more around the IMP plus EDTA in comparison to IMP disk (3,10,16,17).…”

Section: Methodsmentioning

confidence: 99%

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Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Sarhangi

Motamedifar

Sarvari

2013

Jundishapur J Microbiol

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Background: Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa is responsible for many important nosocomial outbreaks including pneumonia and septicemia. Various studies have reported the separation of P. aeruginosa producing MBLs enzymes resulted in increases in multiple traditional antibiotics resistance including carbapenems, cephalosporins and penicillins. Objectives: In this study, based on the standard phenotypic and genotypic methods, we examined the MBLs possible production of a P. aeruginosa isolate. The main objective was exploring the dissemination of resistant MBL P. aeruginosa in south-west of Iran, Shiraz. Materials and Methods: During a six-month period-from October 2011 to March 2012-240 P. aeruginosa isolates, collected from four teaching hospitals in Shiraz located in southwest of Iran, were examined. The isolates were mainly collected from wound, urine, and sputum. Minimum inhibitory concentration (MIC) ≥4 µg/mL to imipenem was determined by micro-dilution broth. Identification of P. aeruginosa with MBL was detected using double disk synergy test (DDST) and polymerase chain reaction (PCR) using specific primers for blaIMP1, blaVIM2, blaSIM1, blaSPM1. All laboratory procedures was according to clinical and laboratory standards institute (CLSI) recommendations. Results: From 240 P. aeruginosa isolates, 82 (34.16%) isolates were imipenem-resistant (minimum inhibitory concentration (MIC) ≥4 µg/ mL). Among these imipenem-resistant isolates, 19 (23.3%) MBL-producing P. aeruginosa isolates were screened using DDST. A specific PCR test confirmed the presence of 18 (21.95%) P. aeruginosa producing blaIMP1 and blaVIM2. Conclusions: Beside our study, the detection of MBL genes were reported in a few studies conducted in Iran. The spread of detected MBLs producing P. aeruginosa were unprecedented in the region due to the lack of independent related researches or the novel incidence of these genes. This detection must be noted by associated clinical and health care services.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Sarhangi

Motamedifar

Sarvari

2013

Jundishapur J Microbiol

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“…The same finding was reported by Shahcheraghi et al where all metallo β-lactamase producing isolates were resistant to Aztreonam ( 28 ). Such mechanisms seem to be also responsible for β-lactam resistance in non-MBL isolates.…”

Section: Discussionsupporting

confidence: 85%

Coexistence of AmpC and Extended-Spectrum β-lactamases in Metallo-β-Lactamase Producing Pseudomonas aeruginosa Burn Isolates in Tehran

Salimi

Eftekhar

2013

Jundishapur J Microbiol

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Background: Multi-drug resistant Pseudomonas aeruginosa causes serious complications in burn patients. One of the most important mechanisms of resistance to β-lactam antibiotics is hydrolysis of antibiotics by various β-lactamases. In recent years, Carbapenems have been widely used for treatment of P. aeruginosa infections. However, the organisms have become resistant to Carbapenems mostly by producing metallo β-lactamases. Objectives: The aim of this study was to determine antibiotic susceptibility, production of extended spectrum and AmpC β-lactamases in metallo β-lactamase producing P. aeruginosa burn isolates. Materials and Methods: Antibiotic susceptibility of 135 P. aeruginosa burn isolates was determined by disc diffusion. Metallo β-lactamase production was screened by the double disc synergy test. Metallo β-lactamase producing bacteria were then tested for extended spectrum β-lactamase production by the combined disc diffusion method. AmpC production was carried out using AmpC disc test. Results: There was 99% resistance to Carbenicillin, and Ticarcillin, 98% to Cotrimoxazole, 96% to Ciprofloxacin, and Aztreonam, 95% to Imipenem, and Meropenem, 94% to Pperacillin, 93% to Tobramycin, 92% to Cefepime, 90% to Amikacin, 89% to Ceftazidime, and 87% to Piperacillin-tazobactam. Among the 128 Imipenem resistant isolates, 32 (25%) were capable of producing metallo β-lactamases of which, 4 (12.5%) produced extended spectrum and 26 (81%) produced AmpC β-lactamases. Four isolates (12.5%) produced all 3 types. Conclusions: This study showed that multiple β-lactamases can be produced in burn isolates. This suggests that use of Cephalosporins and Carbapenems should be restricted in burn isolates to minimize the development and spread of these multidrug resistant pathogens.

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“…1 VIM enzymes were also most prevalent in Korea (88%) and Greece (85%). 22 Previous studies demonstrated that IMP and VIM producer genes of MBLs were also common in Asian studies. [23][24][25] VIM type was also highly prevalent in Turkey.…”

Section: Discussionmentioning

confidence: 99%

Detection Rate of Metallo-β-Lactamase-Expressing Genes; blaVIM-1, blaVIM-2 and blaSPM-1 in Pseudomonas aeruginosa Isolates

Amini¹,

Mobasseri²

2017

Int J Basic Sci Med

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P188 Metallo-beta-lactamase producing strains of Pseudomonas aeruginosa in Iran

Cited by 21 publications

References 0 publications

Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Coexistence of AmpC and Extended-Spectrum β-lactamases in Metallo-β-Lactamase Producing Pseudomonas aeruginosa Burn Isolates in Tehran

Detection Rate of Metallo-β-Lactamase-Expressing Genes; blaVIM-1, blaVIM-2 and blaSPM-1 in Pseudomonas aeruginosa Isolates

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