P10c: A New Biological Control Agent for Control of Fire Blight Which Can Be Sprayed or Distributed Using Honey Bees

Vanneste, J.L.; Cornish, D.A.; Yu, Jiujiu; Voyle, Martyn

doi:10.17660/actahortic.2002.590.33

Cited by 52 publications

(22 citation statements)

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“…Several epiphytic microorganisms show antagonist activity and can exert a certain level of control on the development of E. amylovora if they colonise the stigma's surface prior to the inoculation of the pathogen (Beer et al 1984;Wilson et al 1992;Johnson et al 1993a, b;Wilson and Lindow 1993;Alexandrova et al 2002). For this reason, pollinators can play a crucial role as primary or secondary carriers of biocontrol agents (from now on BCA) (Thomson et al 1992;Vanneste 1996Vanneste , 2002Basim et al 2002;Maccagnani et al 2006). Due to their constant search for newly opened rewarding flowers, pollinators can act as carriers of the BCAs to those flowers which are in the appropriate phenological phase for receiving a preventive inoculum of the selected antagonist.…”

Section: Introductionmentioning

confidence: 99%

Apis mellifera and Osmia cornuta as carriers for the secondary spread of Bacillus subtilis on apple flowers

Maccagnani

Giacomello²,

Fanti³

et al. 2008

BioControl

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The efficiency of two pollinators, Apis mellifera L. (Hymenoptera: Apidae) and the mason bee Osmia cornuta (Latreille) (Hymenoptera: Megachilidae), as carriers of biocontrol agents (BCA) from flower to flower (secondary colonisation) was investigated on apple cv 'Golden Delicious'. The BCA tested was Bacillus subtilis, strain BD170 (BioproÒ) developed for the control of the 'fire blight' caused by Erwinia amylovora (Burril) Winslow et al. The two insect species were studied as secondary BCA carriers on apple plants in pots under net screened tunnels. Their behaviour and capacity to deposit the BCA in the most receptive flower parts were compared both by washing, diluting and plating the flower organs on a recovery medium and by means of PCR analyses based on a molecular marker. O. cornuta showed better performances with respect to A. mellifera. For the field trials, pollinators were introduced in four apple orchards. During apple's flowering, the BD170 (100 g hl -l ) was sprayed once in two fields, and twice in the others. The pollinators' efficacy in carrying the BCA from sprayed flowers to the stigmas of newly opened ones at different times after the spray treatment was evaluated. The detection of the BCA was performed by PCR analysis. The percentages of positive PCR flower samples were higher in the internal treated areas of the fields with respect to the external untreated ones, but the high colonisation level found in the latter and in the flowers opened in both areas several days after the treatment(s) demonstrated that pollinators can play an important role as secondary carriers.

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Section: Introductionmentioning

confidence: 99%

Apis mellifera and Osmia cornuta as carriers for the secondary spread of Bacillus subtilis on apple flowers

Maccagnani

Giacomello²,

Fanti³

et al. 2008

BioControl

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“…Several biological control agents are available commercially for the suppression of fire blight, such as Pseudomonas fluorescens A506 (38), Pantoea agglomerans C9-1 (12), P. agglomerans D325 (26), P. agglomerans Pc10 (36), Bacillus subtilis QST713 (1), and B. subtilis BD170 (5). Biological control agents of fire blight reduce or suppress E. amylovora on floral surfaces, particularly the stigmas, by competition for growth-limiting resources or antibiosis or by excluding the pathogen from infection sites (15,37,38).…”

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confidence: 99%

Improvement of Fitness and Efficacy of a Fire Blight Biocontrol Agent via Nutritional Enhancement Combined with Osmoadaptation

Cabrefiga

Francés

Seguí

et al. 2011

Appl Environ Microbiol

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The efficacy of Pseudomonas fluorescens EPS62e in the biocontrol of Erwinia amylovora was improved by a procedure of physiological adaptation to increase colonization and survival in the phytosphere of rosaceous plants. The procedure consisted of osmoadaptation (OA) and nutritional enhancement (NE). OA was based on saline stress and osmolyte amendment of the growth medium during inoculum preparation. NE consisted of addition of glycine and Tween 80 to the formulation. NE and OA increased the growth rate and carrying capacity of EPS62e under high-relative-humidity (RH) conditions and improved survival at low RH on flowers under controlled environmental conditions. NE did not promote growth or affect infection capacity of E. amylovora. The effect of both methods was tested in the field by following the population of EPS62e using quantitative PCR (Q-PCR) (total population) and CFU counting (culturable population) methods. Following field application, EPS62e colonized blossoms, but it was stressed, as indicated by a sharp decrease in culturable compared to total population levels. However, once established in flowers and at the end of bloom, almost all the total population was culturable. The physiological adaptation treatments increased population levels of EPS62e over those of nonadapted cells during the late stage of the flowering period. Control of fire blight infections in flowers and immature fruits was tested by field application of EPS62e and subsequent inoculation with E. amylovora under controlled-environment conditions. The efficacy of fire blight control increased significantly with the combination of nutritional enhancement and osmoadaptation, in comparison with the absence of physiological adaptation.

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“…Biological control is considered a promising strategy for the management of fire blight and several biological control agents are now commercially available, including P. fluorescens A506 (Wilson and Lindow, 1993), Pantoea agglomerans E325 (Pusey, 1999), B. subtilis QST713 (Aldwinckle et al, 2002), P. agglomerans P10c (Vanneste et al, 2002) and B. subtilis BD170 (Broggini-Schärer et al, 2005) and Pantoea vagans C9-1 (Smits et al, 2010). In vitro -bacterized plantlets not only grew faster than nonbacterized controls but also were sturdier, with a better-developed root system and significantly greater capacities for withstanding biotic (Barka et al, 2000) and abiotic (Bensalim et al, 1998) stresses.…”

Section: Some Beneficial Roles Of Bacterial Strains In Apple Trees Pementioning

confidence: 99%

Microbial Products and Biofertilizers in Improving Growth and Productivity of Apple – a Review

Mosa

Sas-Paszt

Frąc

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tThe excessive use of mineral fertilizers causes many negative consequences for the environment as well as potentially dangerous effects of chemical residues in plant tissues on the health of human and animal consumers. Bio-fertilizers are formulations of beneficial microorganisms, which upon application can increase the availability of nutrients by their biological activity and help to improve soil health. Microbes involved in the formulation of bio-fertilizers not only mobilize N and P but mediate the process of producing crops and foods naturally. This method avoids the use of synthetic chemical fertilizers and genetically modified organisms to influence the growth of crops. In addition to their role in enhancing the growth of the plants, biofertilizers can act as biocontrol agents in the rhizosphere at the same time. Biofertilizers are very safe for human, animal and environment. The use of Azotobacter, Azospirillum, Pseudomonas, Acetobacter, Burkholderia, Bacillus, Paenibacillus and some members of the Enterobacteriaceae is gaining worldwide importance and acceptance and appears to be the trend for the future. 244of plant nutrients and may help to sustain environmental health and soil productivity (O'Connell, 1992). A biofertilizer is a substance which contains living microorganisms which, when applied to seeds, plant surfaces, or soil, colonizes the rhizosphere or the interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to the host plant (Vessey, 2003). Biofertilization is now a very important method for providing the plants with their nutritional requirements without having an undesirable impact on the environment (Abou El-Yazied and Sellim, 2007). Additionally, the use of biofertilizers can improve productivity per unit area in a relatively short time, consume smaller amounts of energy, mitigate contamination of soil and water, increase soil fertility, and promote antagonism and biological control of phytopathogenic organisms (Corpoica et al., 2007). Moreover, biofertilizers are known to improve fixation of nutrients in the rhizosphere, produce growth stimulants for plants, improve soil stability and provide biological control. They also biodegrade substances, recycle nutrients, promote mycorrhiza symbiosis and develop bioremediation processes in soils contaminated with toxic, xenobiotic and recalcitrant substances (Rivera-Cruz et al., 2008). Raghuwanshi (2012) stated that biofertilizers have a great potential as supplementary, renewable and environmental friendly sources of plant nutrients. Furthermore, they are an important component of integrated nutrient management and plant nutrition system. Application of biological potassium fertilizers (BPF), as preparation of silicate bacteria (liquid solution, containing two million bacteria per 1 ml, or packages of 500 g of peat-moss substrate, contains 2 million bacteria) and Azobacterin increased trunk cross-sectional area, mean shoot length, mean leaf area, total leaf area, number of fruits ...

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P10c: A New Biological Control Agent for Control of Fire Blight Which Can Be Sprayed or Distributed Using Honey Bees

Cited by 52 publications

References 0 publications

Apis mellifera and Osmia cornuta as carriers for the secondary spread of Bacillus subtilis on apple flowers

Apis mellifera and Osmia cornuta as carriers for the secondary spread of Bacillus subtilis on apple flowers

Improvement of Fitness and Efficacy of a Fire Blight Biocontrol Agent via Nutritional Enhancement Combined with Osmoadaptation

Microbial Products and Biofertilizers in Improving Growth and Productivity of Apple – a Review

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