“…23 Enzymatic effect of b-1,4-GalT and a-2,3 Sia T in NeuAca2,3-Gal b-1,4 GlcNAc Protocols used to enzymatically synthesize Galb1,4-GlcNAc (4) and NeuAca2,3 Galb1,4-GlcNAc (5) using b1,4-GalT and a2,3-SiaT were performed as published elsewhere. 11,12,24 Compound 3 was tested in an enzymatic reaction with b1,4-GalT in an attempt to prolong the production of 4. The galactosylation reaction was performed with 50 mM HEPES, pH 7.6, 1.0 mM MnCl 2 , 40 mM 3, 20 mM UDP-Gal and 100 mM purified b1,4-GalT.…”