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            Distribution in the Serum Proteins of the Chicken

Clegg, R. E.; Hein, R. E.

doi:10.1126/science.117.3052.714

Cited by 26 publications

(8 citation statements)

References 5 publications

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“…Brandt et al (4) and Clegg et al ( 5 ) studied the differences between the serum proteins of the nonlaying hen and of the laying hen by means of free electrophoresis. Clegg and his collaborators (6,7,8) have also studied the distribution of phosphorus ainong the various electrophoretic fractions and have shown that a significant proportion of the phosphorus in the serum of the laying hen was associated with their electrophoretic component 1, which moved in advance of the serum albumin in their experiments, if it be assumed that their component 2 represents the serum albumin of other workers. Schjeide and Urist (9) and Schjeide, Urist, and McLean (10) have conducted ultracentrifugal studies on the proteins of the fowl's serum and egg yolk.…”

Section: Introductionmentioning

confidence: 96%

A Comparison of Phosvitins Prepared From Hen's Serum and From Hen's Egg Yolk

Mok¹,

Martin²,

Common³

1961

Can. J. Biochem. Physiol.

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A phosvitin has been prepared from the serum of estrogenized laying hens and partially characterized on the basis of its contents of N (12.3y0), P (lO.lTo>, serine (31.O%), and tryptophan (0.56y0). Examination of this serum phosvitin and a yolk phosvitin preparation for N-terminal groups yielded identical chromatographic patterns. Alanine was the major N-terminal amino acid with minor amounts of N-terminal lysine. The sedimentation constant s!,,,=3.7, weightaverage nlolecular weight 4.2X104 (Archibald method), and number-average molecular weight 4.0 X lo4 (calculated from the tryptophan content) of the serum phosvitin were similar t o those of the phosvitin prepared from egg yolk by the same preparative technique. The two preparations behaved similarly when subjected to zone electrophoresis on paper.Zone electrophoretic evidence is submitted for the presence of phosvitin in the supernatant from dilution precipitation of crude lipophosphoprotein from laying hen's serum as well as in the precipitate itself.'Manuscript

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Section: Introductionmentioning

confidence: 96%

A Comparison of Phosvitins Prepared From Hen's Serum and From Hen's Egg Yolk

Mok¹,

Martin²,

Common³

1961

Can. J. Biochem. Physiol.

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“…In many of birds, the onset of laying caused changes in the majority of the constituents in plasma or sera, such as increases in total lipids (15)(16)(17) and total calcium (13,14,18,23) and appearances of serum vitellin (15,16,18,22,23) and a phosphoprotein (15)(16)(17). Estrogenization also caused hyperlipernia (31-39), hypercalce mia (23,31,32,41,44,46,48,50,51), and appearances of serum vitellin (20,49,52) and a phosphoprotein (40, [45][46][47]51). The phosphoprotein was proved to be phosvitin (28,29) which had been found as one of the yolk proteins (53).…”

Section: Discussionmentioning

confidence: 99%

Effect of Estrogenization on the Distribution of Radiomercury in Quail

Nishimura

Urakawa

1972

Jpn.J.Pharmacol

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It is known that mercury derivatives are transported into eggs developing in the reproduc tive organs of birds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)). An egg consists of three components; yolk which develops in the ovary, albumen and shell which are secreted from the site of magnum and the site of uterus of the oviduct, respectively (13). It is known also that alkoxy mercury deriva tives (9, 10) and inorganic mercury (10, 12) are transported mostly into egg yolk, and alkyl mercury compounds into egg albumen (1, 3, 5, 6, 10) and shell (1). The difference in the mode of the transportation may be due to the difference in chemical form among these mercurials (9, 12).
On the other hand, the onset of laying in domestic fowls is accompanied by changes in the majority of the plasma components (13-30). Similar changes in the plasma were induced in immature pullets, nonlaying hens, cockerels, and other birds by treatment with estrogens (20,23,. Large quantities of phospholipids and phosphoprotein in the serum of the laying hen are synthesized in the liver in response to estrogens (20,49,52), and the phospholipids and the phosphoprotein are transported and accumulated into ovarian follicles (20).
Rissanen and Mietinen reported that inorganic mercury or alkoxy mercury derivatives possibly bound to the yolk proteins (9). These forms of mercurials would be transported into ovarian follicles via the circurating blood plasma in a yolk-protein bound form. There is a possibility that retention of mercury in birds may be controlled by treatment with es trogens. In this paper, the effect of estrogenization on distribution of radiomercury is compared between male and immature female quail and laying quail, and interrelationship among all the four entities, i.e., laying plsama, estrogens, plasma radiomercury, and yolk radiomercury, is discussed. METHODSQuail (Coturnix coturnix japonica) used in this experiment were of the JQ-NIBS closed strain and were supplied by the Nippon Institute for Biological Science Tachikawa. Both immature female and adult male quail of 4 and 6 weeks, respectively, were employed.Weight was approx. 90 g each while the laying quail weighed approx. 130 g. Quail which

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“…U SING electrophoresis and ultracentrifugation, investigators (Clegg and Hein, 1953;Heim and Schectman, 1955;Moore, 1948) have noted marked differences between the serum patterns of male and ovulating female chickens. Clegg et al (1951) showed that when a diethylstilbestrol solution was injected into cockerels, changes take place in the serum of these birds similar to changes which occur when hens begin egg production.…”

Section: Introductionmentioning

confidence: 97%