P-Selectin Expression in Cold Preserved Kidneys in University of Wisconsin and Histidine-Tryptophan-Ketoglutarate Solutions

Abstract

The differences and efficacy of standard preservation solutions in kidney transplantation, University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK), remain a topic of debate in recent clinical studies. P-selectins represent glycoproteins expressed on endothelial cells and platelets responsible for the earliest events in ischemia/reperfusion injury in kidney transplantation. This study aimed to compare the levels of P-selectin expression between cold preserved kidney tissues in UW and HTK solutions. Thirty kidneys were procured from male Lewis rats and stored in cold (4°C) solutions for periods of 4, 12, 16, 20, and 24h. Group 1 (n = 15) kidneys were stored in UW solutions, and group 2 (n = 15) kidneys were submerged in HTK solutions. At the end of each time point, the kidneys underwent preparation and levels of Pselectin expression in the tissues were measured using Immunoblot analyses and adjusted volumetric quantification of Western blot signals. For all periods of cold preservation, P-selectin expression was significantly down-regulated in kidney tissues stored in UW compared with HTK solutions (P < 0.001). In summary, UW demonstrated a significant benefit over HTK solution in down-regulating P-selectin expression in cold preserved kidney grafts.

UW is Superior Compared with HTK After Prolonged Preservation of Renal Grafts

“…In our study, 24 h of cold storage revealed tubular damage in renal grafts, which is in accordance with the findings by Worku et al [21]. The magnitude of the tubular damage was more pronounced when grafts were stored in HTK.…”

“…Also, in experimental transplantation, the efficacy of the differing solutions remains a matter of debate, as some studies reported comparable efficacy whereas UW solution was found to be superior in others [20][21][22]. The present study was conducted to compare HTK and UW with regard to cooling properties of solutions, early graft function, tubular damage, and animal survival after renal transplantation.…”

Organ Preservation into the 2020s: The Era of Dynamic Intervention

“…The influence of OPS on the expression of adhesion molecules could provide additional information in relation to recruitment of immune cells in inflammation during organ reperfusion. Comparative studies of HTK and UW solutions have shown much lower expression of P-selectin in the UW solution group, which demonstrates a significant benefit over HTK [46]. …”

External pressure dynamics promote kidney viability and perfusate filtration during ex vivo kidney perfusion

“…The immersion cooling preservation method is now commonly used in clinical practice because of the simplicity of the procedure and transportation. The permissible ischemia time before restarting blood flow once the organ has been harvested from the donor is 4 h for the heart 1 , 8 h for the lungs 2 , 12 h for the liver and small intestine 3 , 4 , and 24 h for the pancreas and kidneys 5 , 6 . Organ transplants from marginal donors, such as cardiac arrest donors or elderly donors, have gradually become mainstream in the recent years to increase the number of donor organs.…”