P‐LME polymer nanocapsules stimulate naïve macrophages and protect them from oxidative damage during controlled drug release

Abstract

Using polymer‐based nanocapsules as novel drug delivery systems have gained significant importance. In this work, we present poly‐(N‐Acryloyl L‐Leucine methyl ester [pLME]) nanocapsules with hollow core (pLME) of size approximately ~160 nm (dia.) synthesized for the first time through the miniemulsion approach. Our study reveals that pLME are biocompatible and immune stimulatory in nature on macrophages, which are decisive for host immunity. These nanocapsules are effective for encapsulating and sustainable release of drug (SNP; nitric oxide donor) at a high extent ~1.04 μM mg‐. pLME also demonstrated their potential of stimulating naïve macrophages toward the M1 phenotype for extended period of time indicating their adjuvant activity on immune system. Our data also demonstrated their antioxidant potential toward H2O2‐induced oxidative stress in macrophages. These results suggest pLME as an effective drug/vaccine delivery material with immune adjuvant and cytoprotective efficacy warranting its pharmacological application. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48363.

Nitric oxide releasing novel amino acid-derived polymeric nanotherapeutic with anti-inflammatory properties for rapid wound tissue regeneration

“…N-Acryloyl glycine monomer (NAG) was synthesized through a modified approach and converted into PNAG NPs. 20,32 The synthesis procedure is elaborated in the ESI. † 2.3.1 Preparation of sodium nitroprusside-loaded poly-(Nacryloyl glycine) nanoparticles.…”

“…N -Acryloyl glycine monomer (NAG) was synthesized through a modified approach and converted into PNAG NPs. 20,32 The synthesis procedure is elaborated in the ESI †…”

In vivo potential of polymeric N-acryloyl-glycine nanoparticles with anti-inflammatory activities for wound healing

“…The N-acryloyl glycine monomer (NAG) was synthesised through a modified approach. [22][23][24] In brief, a required amount of glycine was dissolved in a cold 2 M KOH solution by stirring in a round bottom flask in an ice bath (designated solution 'A'). Then cold 1,4-dioxane was mixed with acryloyl chloride (designated solution 'B').…”

“…The polymeric NAG nanoparticles (PNAG NPs) were synthesised by the mini-emulsion radical polymerisation technique as described in our previous reports 22,23 with modifications according to the need. Briefly, the NAG monomer and hexadecane were dispersed in toluene to form an emulsion of the monomer, where the monomer is present as a dispersed phase (droplets).…”