Ozone oxidation pretreatment for enzymatic saccharification of spent culture media after Lentinula edodes cultivation

Abstract

Ozone oxidation pretreatment was carried out on spent culture media (SCMs) after Lentinula edodes cultivation to improve fermentable sugar production by enzymatic saccharification. SCM samples treated with ozone for different treatment time under various moisture contents (MCs) were enzymatically saccharified, and hydrolysis weight decrease, reducing sugar yield, and monosaccharide yields were determined. Klason lignin content in SCM samples with 20 and 40 % MC was greatly decreased by ozone pretreatment for all ozone treatment time. In addition, in the ozone-treated sample at 40 % MC, the highest hydrolysis weight decrease after saccharification was obtained in the SCM sample treated for 1 h. After saccharification, glucose yield from the ozone-treated sample for 1 h was higher than that treated for 2 h. These results indicate that ozone treatment is effective to improve fermentable sugar production by enzymatic saccharification for the SCMs of L. edodes.

An Effective Modification with Mild Alkali Pretreatment for Enhancing the Biodegradation of Wheat Straw by Pycnoporus sanguineus NFZH-1

“…The E form was cleaved faster than T form under alkaline conditions. Faster E cleavage was explained by steric hindrance and intermolecular hydrogen bonding (Ueda et al 2015), and was consistent with Shimizu's report (Shimizu et al 2012). Table 3 shows the impact of mild alkali pretreatment on lignin biodegradation.…”

On the chemical aspects of the biodelignification of wheat straw with Pycnoporus sanguineus and its combined effects with the presence of Candida tropicalis

“…Shimizu et al (2012) have also reported that the β-O-4 bonds of the E isomer are cleaved faster than those of T isomer under alkaline conditions. Due to steric hindrance and intermolecular hydrogen bonding, T-form is more stable and difficult to be degraded under alkaline conditions (Ueda et al, 2015). T-form degradation is promoted in Ps-Ct fermentation, which could be an important complementarity to alkaline delignification process.…”

Pretratamiento de bagazo de caña de azúcar por ozonólisis para obtención de bioalcoholes: Efectos sobre la liberación de azúcares, la generación de inhibidores y las fermentaciones

“…For red oak treated in a stirred bed reactor, the optimal water content was between 25% and 35% (w/w), with an augmentation of ozone consumption when increasing the water content . Ueda et al (2014) studied the influence of moisture content of shiitake spent culture media (wood meal, Quercus serrata). They obtained the best moisture content value between 20% and 40%; whereas working with water content higher than 60%, lignin was not properly degraded.…”