Ozone influences migration and proliferation of neural stem cells in vitro

Tricarico, Gerardo; Isakovic, Jasmina; Song, Min Suk; Rustichelli, F.; Travagli, Valter; Mitrečić, Dinko

doi:10.1016/j.neulet.2020.135390

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…Previous studies indicate that ozone therapy can be effective in neuromodulation, neuroprotection, and nerve regeneration (14)(15)(16)(17)(18)(19)(20)(21). However, very few studies use the technique for stroke or other low cerebral perfusion syndromes (14,21,22).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of targeted oxidative stress induced by oxygen-ozone gas mixtures in vitro after ischemic induction

Orlandin

Santos

Machado

et al. 2022

Preprint

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Encephalic vascular accident, or stroke, is the most common pathology of the central nervous system in humans. It is the second leading cause of death, as well as physical and cognitive disabilities, in developing countries. It is a vascular disorder that may present in an ischemic (more common) or hemorrhagic form. Ozone is a gas capable of oxidizing double bonds of organic molecules, thereby producing lipoperoxides and aldehydes. Stimulation of the immune and antioxidant system, and improvement in tissue vascularization and oxygenation, are few among several effects exerted by ozone. Ozone therapy has previously been shown to be effective in neuromodulation, neuroprotection, and nerve regeneration. The present study aimed to evaluate the effect of targeted mild ozone after inducing cerebral ischemia in vitro. The experiment was divided into two steps; in the first step, neuroblastoma lineage cells (SH-SY5Y) were subjected to 24 hours of hypoxia in an incubator culture chamber. Treatment with different concentrations of ozone (2–10 μg/mL), followed by an 2,5-diphenyl-2H-tetrazolium bromide assay, indicated a possible neuroregenerative effect at low concentrations. The same protocol was applied to canine amniotic membrane stem cells that were evaluated via colorimetric assay spectrophotometry, fluorescence microscopy, and flow cytometry. The metabolic conditions and cellular regeneration in cells at low ozone concentrations (3–8 μg/mL) correlated with lower levels of apoptosis and oxidative stress compared to cells not subjected to hypoxia. High concentrations of ozone (18–30 μg/mL) promoted an increase in rate of apoptosis and cell death. Therefore, we developed a novel protocol that mimics ozone therapy for ischemic stroke, using ozonized culture medium after hypoxia induction. Although more studies are needed to open new avenues for translational medicine, we conclude that ozone has a dose-dependent hormetic effect and can reverse the effect of ischemia in vitro at low concentrations.

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Section: Introductionmentioning

confidence: 99%

Evaluation of targeted oxidative stress induced by oxygen-ozone gas mixtures in vitro after ischemic induction

Orlandin

Santos

Machado

et al. 2022

Preprint

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“…These data, which are reasonable from a theoretical point of view, remain difficult to analyze and measure due to the complex nature of the reaction types. The ozone derivatives that are formed by a chemical reaction at the level of water-soluble compounds and lipid unsaturation are in turn equipped with an electric charge with an amplitude strong enough to generate a persistent perturbation of the electromagnetic field, with consequent rapid interaction with the cell’s own electric field, its cytoplasmic components, and the tissues of which they are components [ 7 , 8 ]. Irrespective of these facts, gaseous ozone is actually a very toxic substance for all living creatures, but as with all the other substances used in pharmacology, this toxicity is also the principle on which its therapeutic effects are based.…”

Section: Introductionmentioning

confidence: 99%

The Relationship between Ozone and Human Blood in the Course of a Well-Controlled, Mild, and Transitory Oxidative Eustress

Tricarico

Travagli

2021

Antioxidants

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In the last twenty years there has been a proliferation of articles on the therapeutic use of ozone. As it is well-known, the term ozone therapy is very broad. It ranges from either systemic or loco-regional administration of unstable gaseous oxygen/ozone mixtures to the topical application of stable ozonated derivatives. Anyway, in relation to the absence of specific receptors and the extreme reactivity with the biological liquids with which it comes into contact, gaseous ozone cannot be classified as either a drug or a pro-drug. When the gaseous ozone impacts a biological matrix, both reactive oxygen species (ROS) and lipid oxidation products (LOPs) are formed. They represent the effector molecules responsible for modulating the therapeutic activity in the body. Apart from the merits of the action mechanisms resulting from the use of ozone, this article seeks to validate the practice of ozone therapy as an adjuvant treatment in full compliance with the physiology of the whole organism.

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“…Despite many decades of protocol attempts and experimental science, which might even raise some critical opinion about initial empiricism in using medical ozone, physicians have reached a sound overview of how ozone acts on cell systems, resulting effectively in numerous contexts, such as in the SARS-CoV-2 infection and post-COVID (11, 16), in the migration and proliferation of neural stem cells (17), in multiple sclerosis (18,19), in musculoskeletal disorders (20), in chronic fatigue syndrome (21), in retina maculopathy (22), and in knee osteoarthritis (23).…”

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confidence: 99%

The biological activity of medical ozone in the hormetic range and the role of full expertise professionals

Franzini

Valdenassi

Pandolfi

et al. 2022

Front. Public Health

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Ozone influences migration and proliferation of neural stem cells in vitro

Cited by 10 publications

References 26 publications

Evaluation of targeted oxidative stress induced by oxygen-ozone gas mixtures in vitro after ischemic induction

Evaluation of targeted oxidative stress induced by oxygen-ozone gas mixtures in vitro after ischemic induction

The Relationship between Ozone and Human Blood in the Course of a Well-Controlled, Mild, and Transitory Oxidative Eustress

The biological activity of medical ozone in the hormetic range and the role of full expertise professionals

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