Ozone Dosage is the Key Factor of Its Effect in Biological Systems

Abstract

The applications of ozone are not only restricted to environmental remediation or industrial areas. This gas has been applied in medicine to treat several diseases, where positive effects have been confirmed by many clinical studies. According to the European Medical Society of Ozone and the National Center of Scientific Investigation in Cuba, it has not been possible to validate ozone's effectiveness by traditional analytical methods. Thus, this investigation proposed evaluating the effect that ozone has on biological substrates (murine models with induced carcinogenic tumors, inflammation, and wounds), studying the variations that ozone (dissolved in physiological solution or ozonated vegetable oils) provokes over the total unsaturation of lipids (TUL), and by using the so-called method double bond index (DB-index), make a correlation with the dynamic reactions obtained by several analytical methods according to each experimental stage considered in this study.

Chemical characterization of sunflower oil oxidized by UV and ozone with different degrees of oxidation and study of their antimicrobial action

“…This indicated that the peroxides formed during that process are responsible for the biocidal effect and that a longer ozonation times involves the presence of higher amounts of compounds with biocidal activ-ity. This result is consistent with the criterion that the mixture of peroxy compounds formed by ozonation constitutes the active pharmaceutical ingredient in these oils and is in good agreement with previous results (Poznyak et al, 2018). The MIC values obtained for each microorganism are related to their morphological and physiological characteristics.…”

Ozonated Sunflower Oil Exerted Potent Anti-Inflammatory Activities with Enhanced Wound Healing and Tissue Regeneration Abilities against Acute Toxicity of Carboxymethyllysine in Zebrafish with Improved Blood Lipid Profile

“…Interestingly, ozone therapy has been documented to reduce oxidative stress and inflammation, which are the established hallmarks of chronic wounds and, thus, provides a curative potential against chronic wounds [ 7 ]. It has been reported that ozone at 5–60 mg/L concentration is recommended for medical implementations where it exerts various therapeutic effects without having any deleterious ramifications [ 8 , 9 ]. In low doses, ozone stimulates cell protective activity [ 10 ], ameliorates tissue oxygenation, and activates the nuclear factor erythroid 2-related factor 2 (Nrf-2) pathway that regulates the expression of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and other endogenous antioxidants [ 10 , 11 ].…”

Ozone Therapy in Medicine and Dentistry: A Review of the Literature

“…Standardized guidelines and best practices are continuously evolving to establish safe and effective ozone therapy protocols, thereby supporting healthcare practitioners in harnessing the full potential of this innovative treatment modality. By adhering to meticulous concentration control, medical and dental professionals can harness the therapeutic benefits of ozone while safeguarding patient well-being throughout the treatment process [ 10 ].…”