Oyster's cells regulatory volume decrease: A new tool for evaluating the toxicity of low concentration hydrocarbons in marine waters

Naceur, Chiraz Ben; Maxime, Valérie; Mansour, Hédi Ben; Tilly, Véronique Le; Sire, Olivier

doi:10.1016/j.ecoenv.2016.07.030

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…Adaptation of bivalve mollusks to salinity fluctuations is considered to depend on the ability of cells to regulate their volume (Pourmozaffar et al, 2020). However, the presence of cellular osmoregulatory mechanisms in bivalve hemocytes is still under discussion (Naceur et al, 2016; Neufeld & Wright, 1996, 1998; Stara et al, 2020; Torre et al, 2013). To test whether the RVD response of the ark clam hemocytes is oxygen‐dependent hyposmotic stimulation was studied at normoxia when Hb was fully saturated with oxygen, and at hypoxic conditions, when Hb was deoxygenated.…”

Section: Resultsmentioning

confidence: 99%

“…Clearly, long‐term survival of swollen (shrunken) cells requires intracellular adaptive mechanisms, which allow normal functioning in anisosmotic conditions. Digestive cells of Mytilus galloprovincialis , Geukensia demissa, Crassostrea gigas , and hemocytes of Noetia ponderosa showed a short‐term volume regulation in hyposmotic solution (Naceur et al, 2016; Neufeld & Wright, 1996, 1998; Pierce & Warren, 2001; Stara et al, 2020; Torre et al, 2013). On the other hand, gill, mantle, and hemolymph cells of G. demissa did not regulate their volume in conditions of hyposmotic stress (Neufeld & Wright, 1996, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

et al. 2022

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Many bivalve species are considered to be euryhaline organisms due to effective adaptation to fluctuations of environmental salinity. Cellular mechanisms responsible for tolerance to salinity changes remain unclear for bivalves despite this question being critically important for commercially cultured species frequently introduced into regions differing from natural habitat by salinity regime. In the present work laser diffraction method was used for the analysis of volume changes in hemoglobin‐containing ark clam (Anadara kagoshimensis) hemocytes following hyposmotic stimulation. Hemocytes responded to hyposmotic shock (decrease of media osmolarity from 461 to 216 mОsm/L) by a rapid swelling up to 171.5 ± 15.2% of control level. At normal osmotic conditions (osmolarity 461 mOsm/L), hemocyte mean cellular volume (MCV) was 354.0 ± 24.4 fl and maximum MCV of hyposmotically swollen cells prior lysis was 555.5 ± 57.4 fl (at the osmolarity 194 mOsm/L). Ark clam hemocytes demonstrated volume recovery response following hyposmotic swelling. Regulatory volume decrease (RVD) reaction did not depend on hemoglobin confirmation status. Final MCV of swollen hemocytes at the end of experimental period of RVD in oxygenated and deoxygenated suspensions did not significantly differ.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

et al. 2022

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“…In the present study, higher AwGST1 and AwGST2 expressions were mainly found in hepatopancreas, gill and hemocytes that is associated with functions of organs, especially in hpatopancreas. In bivalve, the hepatopancreas is the central metabolic organ and plays an important role in detoxification/degradation of xenobiotics and protection from oxidative stress [49]. It has evidenced that mollusck GSTs can detoxify many plant allelo chemicals and synthetic insecticides [50].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of two glutathione S-transferases from freshwater bivalve Anodonta woodiana : Chronic effects of pentachlorophenol on gene expression profiles

Liu

Shang

et al. 2017

Fish & Shellfish Immunology

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Glutathione S-transferases (GST) play a prominent role in protecting cells against oxidative stress. Our previous study showed that the reactive oxygen species (ROS) generated from pentachlorophenol (PCP) could cause an acute impact on freshwater bivalve Anodonta Woodiana, but its chronic toxicity remain unclear. In order to investigate the chronic effect of PCP, clams A. Woodiana were randomly grouped into PCP treated group in which animals were administrated with 13.9 μg/L concentrations of PCP, and control group those with similar volume dimethyl sulfoxide. In addition, two complete GST sequences were isolated from A. Woodianaa and respectively named AwGST1 and AwGST2. The full-length cDNA of AwGST1 was consisted of a 5' untranslated region (UTR) of 132 bp, a 3' UTR of 80 bp and an open reading frame (ORF) of 609 bp encoding a polypeptide of 203 amino acids. The full-length cDNA of AwGST2 contained a 5' UTR of 57 bp, a 3' UTR of 291 bp and an ORF of 678 bp encoding a polypeptide of 226 amino acids. The constitutive expression levels of AwGST1 and AwGST2 were examined in different tissues including foot, mantle, adductor muscle, heart, hepatopancreas, hemocytes and gill. Administration of PCP could result in a significant increase of AwGST1 and AwGST2 expression in the hepatopancreas, gill and hemocytes. In the hepatopancreas, AwGST1 mRNA levels of PCP treated group increased more than 28.73% at day 1, then 70.37% (P < 0.05) at day 3, reach to 6.64 times (P < 0.01) at day 15 in contrasted with that of control group. AwGST2 increased more 18.18%, 82.88% (P < 0.05) and 2.43 times (P < 0.01) at day 1, 3 and 15, respectively. In the gill, AwGST1 expression showed a significant up-regulation in the PCP treated group during experiment observed compared with that of control group, mRNA level of AwGST2 increased more than 1.44 times (P < 0.05). In addition, expressions of AwGST1 and AwGST2 were significantly induced after PCP treatment in the hemocytes. These results indicated that up-regulations of AwGST1 and AwGST2 expression in bivalve A. woodiana are contribute to against oxidative stress derived from PCP treatment during experiment observed.

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Capacity of tissue water regulation is impaired in an osmoconformer living in impacted estuaries?

David

Lima

Nóbrega

et al. 2018

Ecotoxicology and Environmental Safety

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Oyster's cells regulatory volume decrease: A new tool for evaluating the toxicity of low concentration hydrocarbons in marine waters

Cited by 4 publications

References 51 publications

Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

Isolation and characterization of two glutathione S-transferases from freshwater bivalve Anodonta woodiana : Chronic effects of pentachlorophenol on gene expression profiles

Capacity of tissue water regulation is impaired in an osmoconformer living in impacted estuaries?

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