Oyster Mushroom (Pleurotus ostreatus) Cultivation Using Sawdust and Different Organic Manures

Abstract

Aims: The aim was to evaluate the performance of cultivating oyster mushroom (Pleurotus ostreatus) using sawdust and different organic manures. 
 Study Design: The experimental design used was the complete randomized design (CRD) and the Data obtained was subjected to Analysis of Variance (ANOVA) followed by Turkey’s Least Significant Difference(LSD) test to compare treatment means; differences was considered significant at 95% (P≤0.05) (SPSS Version 21 software).
 Place and Duration of Study: National Biotechnology Development Agency, South East Center, University of Nigeria, Nsukka between September 2019 and November 2019.
 Methodology: Saw dust was prepared as substrate with different organic manures and coded SD, Saw dust; SDP, Sawdust+ Poultry; SDC, Saw dust + cow dung; SDD, Saw dust+ pig dung) in the ratio of 100:0 and 50:50 respectively to cultivate oyster mushroom (Pleurotus ostreatus). Subsequently, the rate of growth, time of harvest, yield and average weight were recorded and proximate composition determined using standard methods.
 Results: Oyster mushroom harvested showed Moisture content ranged (4.63-7.14%), ash content (4.84-6.77%), crude fat (0.98-3.28%), fiber (16.02-18.23%), protein (19.27-33.41%) and carbohydrate (38.18%-48.89%). Average weight yield was highest in saw dust substrate (10.2g) with total yield (980g) and least average weight (7.5g) and total yield (105g) in sawdust and poultry litters substrate. Oyster mushroom from all the substrate differed significantly (p<0.05).
 Conclusion: Sawdust was the most suitable substrate for oyster mushroom cultivation though sawdust and pig dung combination can be a good alternative.

Determination of Nutritional and Biochemical Composition of Selected Pleurotus spps.

“…P3 exhibited ash content of 2.1% while P6, P8, and P1 displayed 5.87%, 7.03%, and 9.14% of ash content, respectively. Our conclusions corroborated the data provided by [ 20 ] for Pleurotus species . According to recent studies [ 21 ], carbohydrate percentages typically range from 65.66 to 82.47%.…”

“…In this study, we found that the contents of crude proteins, fats, carbohydrates, dietary fibers, total ash, and calorie values were in approximate ranges with each other and with the values as reported in previous studies. We concluded that the amounts of crude fats, total ash carbohydrates, proteins, dietary fibers, and calories in this study were within estimated ranges to one and also to the values reported in earlier studies [ 12 , 20 , 21 , 23 , 30 , 31 ]. These results might vary depending on the environmental conditions, substrate used, mushroom species, and drying techniques.…”

“…Protein concentrations of Pleurotus species are listed in Table 4 . The maximal protein concentration of 45.7 ± 0.8 mg/g from Pleurotus species was manifested by P. ostreatus while other species including P. columbinus (P8), P. sajor-caju (P3), and P. sapidus (P6) showed 42.12 ± 0.6 mg/g, 37.93 ± 0.9 mg/g, and 33.47 ± 0.5 mg/g, sequentially [ 20 ]. The nutritional values of edible mushrooms were analyzed comprehensively, and Pleurotus ostreatus showed proximate results to our studies [ 20 ].…”

“…The method outlined by [ 20 ] was used to determine the crude fats in a selected mushroom's dry powder. A thimble containing three grams of dried powder from a particular mushroom was inserted into the extraction tube of the Soxhlet device.…”

“…The crude fiber content of dried mushrooms was calculated using techniques [ 20 ]. 3 g of dried, fat-free sample was obtained, and it was placed in a one-liter beaker.…”