Oxytocin, prostaglandins E and F, estradiol, progesterone, sodium, and potassium in preovulatory bovine follicles either developed normally or stimulated by follicle stimulating hormone

Wise, T.; Vernon, Michael; Maurer, R. R.

doi:10.1016/0093-691x(86)90006-3

Cited by 14 publications

(5 citation statements)

References 37 publications

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“…The concentration of PTGE during ovulation in our study (Table 2) was much higher (484.21 ng/ml) than PTGF concentration (101.01 ng/ml). The marked increase of PTGF and especially PTGE in response to LH surge agrees with observation of other authors made in cow (23, 33, 59, 63).…”

Section: Discussionsupporting

confidence: 92%

“…In addition, Bridges and Fortune (21) demonstrated that both theca and GC of preovulatory follicles are targets for both the PTGF and the PTGE produced by the GC. The results of some earlier studies showed clearly that ovulation process is dependent from follicular prostaglandin production also in cows (23, 47, 53–59). LH surge has been reported to induce enzymes modulating prostaglandin production and specific prostaglandin receptors in the preovulatory follicles (21, 33, 39, 60).…”

Section: Discussionmentioning

confidence: 91%

“…It is well-known that P4 and E2 steroid production of preovulatory follicles change dramatically during the periovulatory period, suggesting them to play an important role during final follicle development and ovulation (11, 14, 23–25, 53, 54). In addition our previous studies demonstrated clear evidence for steroids as local regulators of follicular and luteal activity (4, 19).…”

Section: Discussionmentioning

confidence: 99%

“…In addition our previous studies demonstrated clear evidence for steroids as local regulators of follicular and luteal activity (4, 19). In vivo and in vitro studies demonstrated production and localization of P4 and E2 and their specific receptors in both granulosa and theca cells of perovulatory follicles (11, 14, 23–25). The E2 concentration in the FF of our study (Table 2) was high in the follicle group before and during LH surge, followed by a significant downregulation afterwards.…”

Section: Discussionmentioning

confidence: 99%

“…During these developments in the bovine ovary, steroid hormones and prostaglandins seem to be highly important regulatory mediators playing a central role in the regulation of the estrous cycle (16–22). Progesterone (P4) and estradiol (E2) steroid production of ovulatory follicles change dramatically during the preovulatory period, suggesting them to have an important role during ovulation (19, 23–25). The later stage of follicular development, ovulation and CL formation depends upon growth of new blood vessels (angiogenesis) and the establishment of a functional blood supply (12, 26, 27).…”

Section: Introductionmentioning

confidence: 99%

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Prostaglandins in Superovulation Induced Bovine Follicles During the Preovulatory Period and Early Corpus Luteum

et al. 2019

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The aim of this study was to characterize the regulation pattern of prostaglandin family members namely prostaglandin F2alpha (PTGF), prostaglandin E2 (PTGE), their receptors (PTGFR, PTGER2, PTGER4), cyclooxygenase 2 (COX-2), PTGF synthase (PTGFS), and PTGE synthase (PTGES) in the bovine follicles during preovulatory period and early corpus luteum (CL). Ovaries containing preovulatory follicles or CL were collected by transvaginal ovariectomy ( n = 5 cows/group), and the follicles were classified: (I) before GnRH treatment; (II) 4 h after GnRH; (III) 10 h after GnRH; (IV) 20 h after GnRH; (V) 25 h after GnRH, and (VI) 60 h after GnRH (early CL). In these samples, the concentrations of progesterone (P4), estradiol (E2), PTGF and PTGE were investigated in the follicular fluid (FF) by validated EIA. Relative mRNA abundance of genes encoding for prostaglandin receptors (PTGFR, PTGER2, PTGER4), COX-2, PTGFS and PTGES were quantified by RT-qPCR. The localization of COX-2 and PTGES were investigated by established immunohistochemistry in fixed follicular and CL tissue samples. The high E2 concentration in the FF of the follicle group before GnRH treatment (495.8 ng/ml) and during luteinizing hormone (LH) surge (4 h after GnRH, 574.36 ng/ml), is followed by a significant (P<0.05) downregulation afterwards with the lowest level during ovulation (25 h after GnRH, 53.11 ng/ml). In contrast the concentration of P4 was very low before LH surge (50.64 mg/ml) followed by a significant upregulation ( P < 0.05) during ovulation (537.18 ng/ml). The mRNA expression of COX-2 increased significantely ( P < 0.05) 4 h after GnRH and again 20 h after GnRH, followed by a significant decrease ( P < 0.05) after ovulation (early CL). The mRNA of PTGFS in follicles before GnRH was high followed by a continuous and significant downregulation ( P < 0.05) afterwards. In contrast, PTGES mRNA abundance increased significantely ( P < 0.05) in follicles 20 h after GnRH treatment and remained high afterwards. The mRNA abundance of PTGFR, PTGER2, and PTGER4 in follicles before GnRH was high, followed by a continuous and significant down regulation afterwards and significant increase ( P < 0.05) only after ovulation (early CL). The low concentration of PTGF (0.04 ng/ml) and PTGE (0.15 ng/ml) in FF before GnRH, increased continuously in follicle groups before ovulation and displayed a further significant and dramatic increase ( P < 0.05) around ovulation (101.01 ng/ml, respectively, 484.21 ng/ml). Immunohistochemically, the granulosa cells showed an intensive signal for COX-2 and PTGES in follicles during preovulation and in granulosa-luteal cells of the early CL. In conclusion, our results indicate that the examined bovine prostaglandin family members are involved in the local mechanisms regulating final follicle maturation and ovulation during the folliculo-luteal...

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Section: Discussionsupporting

confidence: 92%