Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c

Ling, Qi; Xu, Xiao; Wei, Xuyong; Wang, Weibing; Zhou, Bin; Wang, Bei; Zheng, Shusen

doi:10.1186/1756-9966-30-66

Cited by 85 publications

(65 citation statements)

References 21 publications

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“…Bcl-2/Bax heterodimers were increased and eliminated the pro-apoptotic role of Bax along with the Bcl-2 protein expression were increased, so the role of inhibits apoptosis were enhanced. On the other hand, Bax/Bax homodimers were increased when Bax protein expression were increased, the role of pro-apoptotic were enhanced [11,12]. The results of this study show that after A549 cells treated with 2-15 mmol/L oxymatrine for 48 h, Bax protein expression were increased significantly compared with the negative control; on the contrary, the expression of Bcl-2 were reduced significantly.…”

Section: Discussionsupporting

confidence: 44%

Oxymatrine inhibited cell proliferation by inducing apoptosis in human lung cancer A549 cells

Wang

Han

Zhu

2015

BME

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Abstract.To investigate the inhibition effect of oxymatrine induces human lung cancer A549 cells apoptosis. The A549 cells were cultured for 24 h, than the various concentration of oxymatrine (2 mmol/L, 4 mmol/L, 8 mmol/L, 15 mmol/L) were added into different experimental group cells, and 5-fluorouracil were added into the positive control group cells for 12 h, 24 h, 36 h, 48 h respectively. The A549 cells inhibition rate, apoptosis, and the expression of Bcl-2 and Bax were examined by MTT method, Annexin /PI double staining method, real-time quantitative PCR and western blot, respectively. At same time, the morphological changes of A549 cells were observed with an inverted microscope. In the range of 2 mmol/L~15 mmol/L, oxymatrine had obvious inhibition effects on the proliferation of A549 cells. Compared with the negative control group, it has significantly different (P<0.01). There was remarkably the time-and dose-dependent correlation. After A549 cells were treated with 8 mmol/L oxymatrine for 24 h, the morphological change of cell apoptosis was observed and the extent of apoptosis was quantified by flow cytometry. Furthermore, the expression of Bcl-2 was reduced and the expression of Bax was increased remarkably (P<0.05). Oxymatrine has significant inhibition effects on the cells proliferation and the effects showed time-dependent and dose-dependent. Oxymatrine can induce apoptosis of the A549 cells by regulating the expression of Bcl-2 and Bax.

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Section: Discussionsupporting

confidence: 44%

Oxymatrine inhibited cell proliferation by inducing apoptosis in human lung cancer A549 cells

Wang

Han

Zhu

2015

BME

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“…Oxymatrine can also induce apoptosis in human pancreatic cancer PANC-1 cells by a mechanism similar to matrine. After treatment with oxymatrine, livin and survivin genes were down-regulated and the Bax/Bcl-2 ratio was increased, accompanied by the release of mitochondrial cytochrome c and the activation of caspase-3 protein in PANC-1 cells [24] . It is also suggested that oxymatrine combined with angiogenesis inhibitor NM-3 has a synergistic inhibitory effect on the growth of human gastric cancer SGC-7901, MKN-45, and MKN-74 cells in a time-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor activities of active ingredients in Compound Kushen Injection

Wang¹,

et al. 2015

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Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are matrine (MT) and oxymatrine (OMT) that exhibit a variety of pharmacological activities, including anti-inflammatory, anti-allergic, anti-viral, anti-fibrotic and cardiovascular protective effects. Recent evidence shows that these compounds also produce anti-cancer actions, such as inhibiting cancer cell proliferation, inducing cell cycle arrest, accelerating apoptosis, restraining angiogenesis, inducing cell differentiation, inhibiting cancer metastasis and invasion, reversing multidrug resistance, and preventing or reducing chemotherapy-and/or radiotherapy-induced toxicity when combined with chemotherapeutic drugs. In this review, we summarize recent progress in studying the anti-cancer activities of MT, OMT and CKI and their potential molecular targets, which provide clues and references for further study.

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“…The study reported that EEHDW inhibited the growth of HT-29 cells by inducing cell morphological changes, which reduce the cell viability due to DNA fragmentation and loss of plasma membrane asymmetry, and also the collapse of mitochondrial membranes, the activation of caspase-9 and caspase-3 and the increase of the pro-apoptotic Bax to anti-apoptotic Bcl-2 ratio. Oxymatrine (19) is an isolated extract from the traditional Chinese herb Sephora flavescent Ait, and its anticancer effect was examined on human pancreatic cancer PANC-1 cells. The results showed its efficacy by releasing cytochrome c and activating caspase-3 proteins.…”

Section: Inducing Cellular Apoptosismentioning

confidence: 99%