Oxygen-induced genetic changes in dry yeast cells

Abstract

Cells of Saccharomyces cerevisiae were dried in vacuum, exposed to oxygen, nitrogen, air, and water vapor, and rehydrated with degassed medium without exposure to air. Drying per se caused few genetic changes, but the exposure of dry cells to oxygen increased the frequency of adenine-requiring colonies.

Pre‐treatment with D942, a furancarboxylic acid derivative, increases desiccation tolerance in an anhydrobiotic tardigrade Hypsibius exemplaris

“…For example, Pereira et al [17] demonstrated that yeast cells were oxidized more than 10-fold by desiccation. Desiccated organisms kept in a vacuum or a reduced oxygen environment such as nitrogen recovered well compared to those kept in normal air or oxygen environment, suggesting that exogenous oxidation is a damaging factor during anhydrobiosis [18][19][20]. On the other hand, there were two downregulated proteins.…”