Oxidised mannan antigen conjugates preferentially stimulate T1 type immune responses

McKenzie, Ian F. C.; Apostolopoulos, Vasso; Lees, Catherine; Xing, Pei‐Xiang; Lofthouse, Shari; Osinski, Carla; Popovski, Violeta; Acres, Bruce; Pietersz, G. A.

doi:10.1016/s0165-2427(98)00094-4

Cited by 26 publications

(19 citation statements)

References 7 publications

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“…Thus, this substance may be considered as a stranger compound to the host and result in increased immune response. The immune response is mediated by macrophages activation since macrophages have mannans binding receptors, as demonstrated by McKenzie et al (1998).…”

Section: Resultsmentioning

confidence: 99%

Effect of mannanoligosaccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease viruses

Oliveira

Figueiredo-Lima

Filho³

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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The effect of including mannanoligosaccharides (MOS) and/or enzymes in broiler diets on antibody titers against infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV) was evaluated. A total of 750 broilers were distributed into a completely randomized experimental design in a factorial arrangement 2 x 2 + 1 with two levels of MOS (0 and 0.1% until 21 days and 0.05% from 22 to 42 days of age), two levels of enzymes (0 and 0.05%) and a positive control diet containing antibiotic, totaling five treatments with five replicates each. For antibody analyses, blood samples were weekly collected by jugular vein puncture in the same two birds per replicate. The first and last collections were done at 7 and 42 days of age, respectively. The inclusion of MOS resulted in increased antibody titers against IBDV in the fourth (P<0.03) and fifth (P<0.02) weeks, and against NDV in the third (P<0.01), fourth (P<0.03) and fifth (P<0.03) weeks of age. MOS was effective in stimulating the humoral immune responses against IBDV and NDV vaccine viruses.Keywords: broiler, additive, humoral immune modulation, prebiotic (0 e RESUMO O efeito da inclusão de mananoligossacarídeo (MOS) e/ou enzimas em dietas de frangos sobre os títulos de anticorpos contra os vírus das doenças de Gumboro (VDG) e de Newcastle (VDN). Setecentos e cinqüenta aves foram distribuídas em um delineamento experimental inteiramente ao acaso, em arranjo fatorial 2 x 2 + 1, com dois níveis de MOS

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Section: Resultsmentioning

confidence: 99%

Effect of mannanoligosaccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease viruses

Oliveira

Figueiredo-Lima

Filho³

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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“…In vivo , a mature phenotype of lymph node and splenic DCs were induced through a TLR-4-dependent mechanism [50]. In mice, mannose- or reduced mannan-coupled antigens stimulated Th2 type responses with IL-4 production whereas oxidized mannan-coupled antigens stimulated Th1 responses with IFN-γ, IL-2 and IL-12 production [51]. Mannan, oxidatively coupled to recombinant protein antigen and given intranasally was shown to be superior to cholera toxin in enhancing the production of antigen-specific IgA, IgG1 and IgG2a in serum, and secretary IgA in lung, tear duct, vaginal and salivary secretions [52].…”

Section: Mannansmentioning

confidence: 99%

Carbohydrate-based immune adjuvants

Petrovsky

Cooper

2011

Expert Review of Vaccines

133

102

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The role for adjuvants in human vaccines has been a matter of vigorous scientific debate, with the field hindered by the fact that for over 80 years, aluminum salts were the only adjuvants approved for human use. To this day, alum-based adjuvants, alone or combined with additional immune activators, remain the only adjuvants approved for use in the USA. This situation has not been helped by the fact that the mechanism of action of most adjuvants has been poorly understood. A relative lack of resources and funding for adjuvant development has only helped to maintain alum’s relative monopoly. To seriously challenge alum’s supremacy a new adjuvant has many major hurdles to overcome, not least being alum’s simplicity, tolerability, safety record and minimal cost. Carbohydrate structures play critical roles in immune system function and carbohydrates also have the virtue of a strong safety and tolerability record. A number of carbohydrate compounds from plant, bacterial, yeast and synthetic sources have emerged as promising vaccine adjuvant candidates. Carbohydrates are readily biodegradable and therefore unlikely to cause problems of long-term tissue deposits seen with alum adjuvants. Above all, the Holy Grail of human adjuvant development is to identify a compound that combines potent vaccine enhancement with maximum tolerability and safety. This has proved to be a tough challenge for many adjuvant contenders. Nevertheless, carbohydrate-based compounds have many favorable properties that could place them in a unique position to challenge alum’s monopoly over human vaccine usage.

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“…In general, antigen‐presenting cells (APC) are not efficient in uptake and processing of exogenous antigens through the MHC class I pathway 2 . In addition to the use of conventional adjuvants such as Quil A, liposomes or virus‐like particles to improve the intracellular access of proteins and peptides into the cell, some delivery systems utilize targeting to receptors on APC such as mannose or DEC‐205 receptors 3–15 …”

Section: Introductionmentioning

confidence: 99%

Penetratin tandemly linked to a CTL peptide induces anti‐tumour T‐cell responses via a cross‐presentation pathway

2006

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Summary Recently there has been increasing evidence to suggest that membrane translocating peptides enter cells by a receptor‐dependent pathway. There have been some studies on the mechanism of major histocompatibility complex (MHC) class I presentation of membrane translocating peptides incorporating cytotoxic T lymphocyte epitopes. However, these have been on different cell lines and only a limited number of inhibitors of the antigen presentation pathway were used. Herein, we demonstrate a comprehensive study utilizing a full spectrum of inhibitors to various pathways of MHC class I to elucidate the mechanism of the membrane translocating peptide, penetratin from Antennapedia (Int). It is clear that Int, RQIKIWFQNRRMKWKK when tandemly linked to a cytotoxic T lymphocyte peptide of ovalbumin, SIINFEKL (IntSIIN) is endocytosed via phagocytosis or macropinocytosis by dendritic cells in an ATP‐dependent manner and is processed by a proteasome‐ and tapasin‐independent pathway for presentation and loading to MHC class I molecules. In addition, the majority of antigen is taken up by negatively charged receptors. IntSIIN activates T cells in vitro and in vivo and protects mice against challenge with an ovalbumin‐expressing tumour.

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Oxidised mannan antigen conjugates preferentially stimulate T1 type immune responses

Cited by 26 publications

References 7 publications

Effect of mannanoligosaccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease viruses

Effect of mannanoligosaccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease viruses

Carbohydrate-based immune adjuvants

Penetratin tandemly linked to a CTL peptide induces anti‐tumour T‐cell responses via a cross‐presentation pathway

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