Oxide nanowire microfluidics addressing previously-unattainable analytical methods for biomolecules towards liquid biopsy

Takahashi, H.; Baba, Yoshinobu; Yasui, Takahiro

doi:10.1039/d1cc05096f

Cited by 11 publications

(6 citation statements)

References 102 publications

(115 reference statements)

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“…Therefore researchers have recently started integrating different methods based on the physical properties of exosomes with the design of the microfluidic device. Thus, microfluidics-based isolation techniques have developed and evolved over the years [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. The following sections will discuss some of the most interesting methods mostly based on the immunoaffinity approaches.…”

Section: Isolation and Detection Techniquesmentioning

confidence: 99%

Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review

et al. 2022

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Extracellular vesicles (EVs) are a group of communication organelles enclosed by a phospholipid bilayer, secreted by all types of cells. The size of these vesicles ranges from 30 to 1000 nm, and they contain a myriad of compounds such as RNA, DNA, proteins, and lipids from their origin cells, offering a good source of biomarkers. Exosomes (30 to 100 nm) are a subset of EVs, and their importance in future medicine is beyond any doubt. However, the lack of efficient isolation and detection techniques hinders their practical applications as biomarkers. Versatile and cutting-edge platforms are required to detect and isolate exosomes selectively for further clinical analysis. This review paper focuses on lab-on-chip devices for capturing, detecting, and isolating extracellular vesicles. The first part of the paper discusses the main characteristics of different cell-derived vesicles, EV functions, and their clinical applications. In the second part, various microfluidic platforms suitable for the isolation and detection of exosomes are described, and their performance in terms of yield, sensitivity, and time of analysis is discussed.

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Section: Isolation and Detection Techniquesmentioning

confidence: 99%

Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review

et al. 2022

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“…Another possible option is the application of photoactive materials (e. g. metal oxide semiconductors) as supports. In this case, fabrication of the materials into micro/nanoarrays appears to be highly effective in enhancing light absorption [104] and is applicable for various materials [86,87,92,105,106] . Furthermore, VFDs hold advantages over conventional channel‐like flow reactors as they are able to transfer the input fluid into a thin film climbing the inner surface ascribing to the high rotation speed.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Diverse Supports for Immobilization of Catalysts in Continuous Flow Reactors

Lin

Qiu

2022

Chemistry A European J

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The rapid development of continuous flow processes is driving innovations in various chemical syntheses and industrial productions. Immobilizing catalysts in flow reactors allows transformations with high-efficiency and excludes the subsequent separation procedures. This concept outlines the approaches to incorporate catalysts within flow reactors, with particular focus on the application of additional supports including inorganic materials like silica, zeolite and reduced graphene oxide, polymeric materials like polymer packings, monoliths, cross-linked gels and polymer brushes, and other materials for specific conditions like transparent glass fibers and glass beads. Furthermore, advanced methods to develop ordered micro-/nanoarrays from internal walls of flow channels for immobilization of catalysts as well as application of innovative vortex fluidic devices are discussed to inspire new designs of supports for novel fluidic reactors with broad applications.

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“…Taking the viewpoint of the advantages of high efficiency in capturing EVs using nanowires, 26 we have considered the potential application of nanowires in cancer immunotherapy in vitro. We have previously reported on the use of nanowires for early cancer diagnosis through the capture and analysis of EVs from urine, 27−29 and a high efficiency in a charge-based manner has been obtained.…”

Section: ■ Introductionmentioning

confidence: 99%

On-Site Stimulation of Dendritic Cells by Cancer-Derived Extracellular Vesicles on a Core–Shell Nanowire Platform

Zhang,

Ono,

Kawaguchi

et al. 2024

ACS Appl. Mater. Interfaces

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Extracellular vesicles (EVs) contain a subset of proteins, lipids, and nucleic acids that maintain the characteristics of the parent cell. Immunotherapy using EVs has become a focus of research due to their unique features and bioinspired applications in cancer treatment. Unlike conventional immunotherapy using tumor fragments, EVs can be easily obtained from bodily fluids without invasive actions. We previously fabricated nanowire devices that were specialized for EV collection, but they were not suitable for cell culturing. In this study, we fabricated a ZnO/ Al 2 O 3 core−shell nanowire platform that could collect more than 60% of the EVs from the cell supernatant. Additionally, we could continue to culture dendritic cells (DCs) on the platform as an artificial lymph node to investigate cell maturation into antigenpresenting cells. Finally, using this platform, we reproduced a series of on-site immune processes that are among the pivotal immune functions of DCs and include such processes as antigen uptake, antigen presentation, and endocytosis of cancer-derived EVs. This platform provides a new ex vivo tool for EV-DC-mediated immunotherapies.

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Oxide nanowire microfluidics addressing previously-unattainable analytical methods for biomolecules towards liquid biopsy

Abstract: Nanowire microfluidics using a combination of self-assembly and nanofabrication technologies is expected to provide bioanalytical methods for liquid biopsy, which are impossible to achieve with conventional technologies.

Cited by 11 publications

References 102 publications

Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review

Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review

Diverse Supports for Immobilization of Catalysts in Continuous Flow Reactors

On-Site Stimulation of Dendritic Cells by Cancer-Derived Extracellular Vesicles on a Core–Shell Nanowire Platform

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