Oxidative stress response in the skin mucus layer of Goodea gracilis (Hubbs and Turner, 1939) exposed to crude oil: A non-invasive approach

Dzul-Caamal, Ricardo; Salazar‐Coria, L.; Olivares-Rubio, Hugo F.; Rocha-Gómez, Maria Alejandra; Girón-Pérez, Manuel Iván; Vega-López, Armando

doi:10.1016/j.cbpa.2016.05.008

Cited by 25 publications

(11 citation statements)

References 57 publications

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“…We considered statistical signi cance at a value of p ≤ 0.05 for all tests. With the aim to compare the effects elicited by M. aeruginosa extract with those damages induced by the positive control of PP1/PP2A inhibition (norcantharidine) on the CNS organs, we employed the "Integrated Biological Response version 2" (IBRv2) analysis developed by Sanchez et al (2013), which is a helpful tool in the interpretation of these variables under both eld and controlled conditions (Olivares-Rubio et al 2013;Dzul-Caamal et al 2016). Also, we applied a parametric correlation analysis (Pearson) to know the possible relationships among neurotoxicity biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis Aeruginosa Extract: An Uncommon Consequence of the Eutrophication Process

Nájera-Martínez

Landon-Hernández

Romero-López

et al. 2021

Preprint

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Microcystins (MCs) are produced during the growth and proliferation of some species of cyanobacteria, mainly Microcystis aeruginosa, which has massive growth in eutrophic water bodies. Microcystins are highly toxic Microcystis-derived metabolites that exert its main effect in the liver through the inhibition of protein phosphatase (PP1 and PP2A). However, other damages in fish species are less documented and could be unexpected. The aim of the current study was to evaluate the effects of Microcystis aeruginosa extract (MaE) into the central nervous system (CNS) of the Nile tilapia. The MaE was normalized by MCs content (MC-LR). We include a positive control for protein phosphatase inhibition, norcantharidin intraperitoneally dosed at sublethal levels. On the eighth day, measurement of neurotransmission biomarkers (AChE, BChE, CbE and GABA) were measured, as well as levels of mitochondrial calcium, and the mitochondrial membrane potential by flow cytometry in the brain and spinal cord were assessed, in addition to the PP1/PP2A activity in the liver. The MCs elicited mortality at 5 µg/L. The positive control and MCs at sublethal levels inhibited the PP1/PP2A activity in the liver and induced alterations in the neurotoxicity biomarkers evaluated in the CNS. This response is probably due to the disruption of transport ions, dependent and independent of ATP because of alterations in the mitochondrial membrane potential and mitochondrial calcium. The findings of this study suggest that pollutants capable of inducing cyanobacterial blooms are able, in an indirect way, to exert neurotoxic effects in fish species through MC levels.

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Section: Discussionmentioning

confidence: 99%

Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis Aeruginosa Extract: An Uncommon Consequence of the Eutrophication Process

Nájera-Martínez

Landon-Hernández

Romero-López

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis aeruginosa Extract: an Uncommon Consequence of the Eutrophication Process

Nájera-Martínez

Landon-Hernández

Romero-López

et al. 2021

Water Air Soil Pollut

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Microcystins (MCs) are produced during the growth and proliferation of some species of cyanobacteria, mainly Microcystis aeruginosa, which has massive growth in eutrophic water bodies. Microcystins are highly toxic Microcystisderived metabolites that exert its main effect in the liver through the inhibition of protein phosphatase (PP1 and PP2A).However, other damages in sh species are less documented and could be unexpected. The aim of the current study was to evaluate the effects of Microcystis aeruginosa extract (MaE) into the central nervous system (CNS) of the Nile tilapia. The MaE was normalized by MCs content (MC-LR). We include a positive control for protein phosphatase inhibition, norcantharidin intraperitoneally dosed at sublethal levels. On the eighth day, measurement of neurotransmission biomarkers (AChE, BChE, CbE and GABA) were measured, as well as levels of mitochondrial calcium, and the mitochondrial membrane potential by ow cytometry in the brain and spinal cord were assessed, in addition to the PP1/PP2A activity in the liver. The MCs elicited mortality at 5 µg/L. The positive control and MCs at sublethal levels inhibited the PP1/PP2A activity in the liver and induced alterations in the neurotoxicity biomarkers evaluated in the CNS.This response is probably due to the disruption of transport ions, dependent and independent of ATP because of alterations in the mitochondrial membrane potential and mitochondrial calcium. The ndings of this study suggest that pollutants capable of inducing cyanobacterial blooms are able, in an indirect way, to exert neurotoxic effects in sh species through MC levels.

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“…A sample of 1 L of exposure medium was collected after the snails were euthanized. The quantification of PAHs was undertaken with a Biotek SynergyMX spectrofluorometer, using certified analytical standards obtained from Chem Service, Inc. (West Chester, PA), as previously documented (Dzul-Caamal et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…Analyses were done in GraphPad Prism version 5.00 for Windows (GraphPad Software, San Diego, California, USA, www.graphpad.com). To determine the impact of the WAF of MCO in tissues of tegogolos, we calculated the IBRv2 according to Sanchez et al (2013) and employed the general IBRv2 (gIBRv2) proposed by Dzul-Caamal et al (2016) to integrate the IBRv2 values by tissue and treatment.…”

Section: Methodsmentioning

confidence: 99%

Oxidative stress, lipid metabolism, and neurotransmission in freshwater snail (Pomacea patula) exposed to a water-accommodated fraction of crude oil

2017

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Background. Crude oil is a super mixture of chemical compounds and is commonly found in aquatic environments. The tegogolo (Pomacea patula Baker, 1922) is a Mexican freshwater snail endemic to Lake Catemaco in Veracruz; currently, however, its distribution has expanded to many freshwater ecosystems that suffer the impact of crude oil spills and oil byproducts like fuels. Goals. To assess a series of biomarkers involved in oxidative stress, neurotransmission, and fatty acid metabolism in tegogolos exposed to the water-accommodated fraction (WAF) of Maya crude oil (MCO). Methods. Tegogolo specimens were exposed to WAF of MCO obtained from loads of 0.1, 1, 10 and 100 mg/L. We evaluated ROS (O 2-* and H 2 O 2), oxidative stress (TBARS and RC=O), enzymes involved in antioxidant defense (SOD, CAT, and GPx), some enzymes involved in neurotransmission (AChE, GDA, and CbE activities), and biomarkers of fatty acids metabolism (fatty acids levels and AOX activity). Results. Clear biomarkers responses were observed only in some tissues. ROS were clearly higher than controls in the foot, head, and kidney; however, others biomarkers of oxidative stress remain statistically unchanged. SOD response was irregular with respect to controls and treatments. In contrast, CAT (foot) and GPx (foot and intestine) were the more active enzymes and their activities were higher than in controls. The responses of some enzymes involved in neurotransmission suggest that compensation mechanisms exist between AChE and GDA in the foot and head. Fatty acids metabolism increased with exposure to WAF; however, these types of biomarkers seem unsuitable for monitoring the toxic effects produced by WAF at low environmental concentrations. Conclusions. We can conclude that under the exposure conditions discussed herein, the tegogolos showed acclimation to WAF of Maya crude oil by complex mechanisms.

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Oxidative stress response in the skin mucus layer of Goodea gracilis (Hubbs and Turner, 1939) exposed to crude oil: A non-invasive approach

Cited by 25 publications

References 57 publications

Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis Aeruginosa Extract: An Uncommon Consequence of the Eutrophication Process

Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis Aeruginosa Extract: An Uncommon Consequence of the Eutrophication Process

Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis aeruginosa Extract: an Uncommon Consequence of the Eutrophication Process

Oxidative stress, lipid metabolism, and neurotransmission in freshwater snail (Pomacea patula) exposed to a water-accommodated fraction of crude oil

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