Disruption of Neurotransmission, Membrane Potential, and Mitochondrial Calcium in the Brain and Spinal Cord of Nile Tilapia Elicited by Microcystis aeruginosa Extract: an Uncommon Consequence of the Eutrophication Process

Abstract

Microcystins (MCs) are produced during the growth and proliferation of some species of cyanobacteria, mainly Microcystis aeruginosa, which has massive growth in eutrophic water bodies. Microcystins are highly toxic Microcystisderived metabolites that exert its main effect in the liver through the inhibition of protein phosphatase (PP1 and PP2A).However, other damages in sh species are less documented and could be unexpected. The aim of the current study was to evaluate the effects of Microcystis aeruginosa extract (MaE) into the central nervous system (CNS) of the Nile tilapia. The MaE was normalized by MCs content (MC-LR). We include a positive control for protein phosphatase inhibition, norcantharidin intraperitoneally dosed at sublethal levels. On the eighth day, measurement of neurotransmission biomarkers (AChE, BChE, CbE and GABA) were measured, as well as levels of mitochondrial calcium, and the mitochondrial membrane potential by ow cytometry in the brain and spinal cord were assessed, in addition to the PP1/PP2A activity in the liver. The MCs elicited mortality at 5 µg/L. The positive control and MCs at sublethal levels inhibited the PP1/PP2A activity in the liver and induced alterations in the neurotoxicity biomarkers evaluated in the CNS.This response is probably due to the disruption of transport ions, dependent and independent of ATP because of alterations in the mitochondrial membrane potential and mitochondrial calcium. The ndings of this study suggest that pollutants capable of inducing cyanobacterial blooms are able, in an indirect way, to exert neurotoxic effects in sh species through MC levels.

Norcantharidin Nanoemulsion Development, Characterization, and In Vitro Antiproliferation Effect on B16F1 Melanoma Cells

“…The exceeding dye was discarded and washed with 500 µL of a 1.0% acetic acid solution. Finally, 1.0 mL of 10 mM TRIS solution (pH 10.5) was added, and the dye uptake was measured by absorbance (Abs) reading at 550 nm (spectrophotometry, Synergy MX microplate reader, BioTek) [48]. The half-maximal inhibitory concentration (IC 50 ) was predicted for cell mortality, calculated according to Equation ( 5) for colorimetric assays [39]: % Cell viability = Abs Samples − Abs Blank Abs Control − Abs CtrlBlk × 100.…”