Oxidative stress parameters in type I, type II and insulin-treated type 2 diabetes mellitus; insulin treatment efficiency

Seghrouchni, Ilham; Drai, Jocelyne; Bannier, Edith; Rivière, Joël; Calmard, Pascale; Garcia, Isabelle; Orgiazzi, Jacques; Revol, A

doi:10.1016/s0009-8981(02)00099-2

Cited by 176 publications

(112 citation statements)

References 29 publications

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“…It is present in both type 1 and type 2 diabetes and plays a key role in the development of complications through different and multiple mechanisms, including nonenzymatic glycation [6], protein kinase C activation [7], and increased aldose reductase activity [8;9]. There is accumulating evidence, especially in diabetic animal models (such as streptozotocin-diabetic rats and mice), that hyperglycemia induces oxidative stress, and reactive species such as lipid peroxides and glucose-derived aldehydes contribute to the pathogenesis of the disease and the development of complications as well [10][11][12][13][14][15]. Various studies propose different sites for production of reactive species in diabetes.…”

Section: Introductionmentioning

confidence: 99%

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Stadler

Bonini

Dallas

et al. 2008

Free Radical Biology and Medicine

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Free radical production is implicated in the pathogenesis of diabetes mellitus, where several pathways and different mechanisms were suggested in the pathophysiology of the complications. In this study, we used electron paramagnetic resonance (EPR) spectroscopy combined with in vivo spin-trapping techniques to investigate the sources and mechanisms of free radical formation in streptozotocininduced diabetic rats. Free radical production was directly detected in the diabetic bile, which correlated with lipid peroxidation in the liver and kidney. EPR spectra showed the trapping of a lipidderived radical. Such radicals were demonstrated to be induced by hydroxyl radical through isotope labeling experiments. Multiple enzymes and metabolic pathways were examined as the potential source of the hydroxyl radicals using specific inhibitors. Neither xanthine oxidase, cytochrome P450s, the Fenton reaction, nor macrophage activation were required for the production of radical adducts. Interestingly, inducible nitric oxide synthase (apparently uncoupled) was identified as the major source of radical generation. The specific iNOS inhibitor 1400W as well as L-arginine pretreatment reduced the EPR signals to baseline levels, implicating peroxynitrite as the source of hydroxyl radical production. Applying immunological techniques, we localized iNOS overexpression in the liver and kidney of diabetic animals, which was closely correlated with the lipid radical generation and 4-hydroxynonenal-adducted protein formation, indicating lipid peroxidation. In addition, protein oxidation to protein free radicals occurred in the diabetic target organs. Taken together, our studies support inducible nitric oxide synthase as a significant source of EPR-detectable reactive intermediates, which leads to lipid peroxidation and may contribute to disease progression as well.

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Section: Introductionmentioning

confidence: 99%

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Stadler

Bonini

Dallas

et al. 2008

Free Radical Biology and Medicine

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“…Knowledge of its heterogeneous phenotypes is on the rise [2] . Diabetes is recognized as one of the leading causes of morbidity and mortality in the world; about 2.5-7% of the world's population are diagnosed with diabetes mellitus [3] . Despite of the significant effect of anti-hyperglycemic drugs and insulin sensitizers, there remains side effects that necessitate finding other alternatives.…”

Section: Introductionmentioning

confidence: 99%

In vivo, Acute, Normo-hypoglycemic, Antihyperglycemic, Insulinotropic Actions of Orally Administered Ethanol Extract of Citrullus colocynthis (L.) Schrab Pulp

Dallak¹,

Alkhateeb²,

Abbas³

et al. 2009

American J. of Biochemistry and Biotechnology

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Problem statement: Citrullus Colocynthis (L.) Schrab (cucurbitaceae family) (Handal) is a fruit commonly known as bitter apple or bitter cucumber. Traditionally, Citrullus colocynthis has been used for the treatment of diabetes. In particular, the acute effect and rout of administration of ethanol extract of the seedless pulp in vivo remains untested. We investigated the effect of different routs of administration and their hypoglycemic and insulinotropic effects of ethanol/water (20/80 V/V) extract of the dried seedless pulp of Citrullus colocynthis (L.) Schrab on normal and alloxan-induced diabetic rats. Approach: Rats were divided into two groups, normal and diabetic rats. Non-diabetic rats were fasted for 18 h before the beginning of the experimental procedure. About 250 µL of normal saline (i.e., vehicle) was administered to control groups either i.p. (Group I) or orally (Group III) using modified feeding canula. As 250 µL pulp extract was administered i.p. (Group II) or orally (Group IV). Five min, after the administration of the extract or vehicle, an Intraperitoneal Glucose Tolerance Test (IPGTT) was conducted (1.5 g glucose/kg) on groups I, II, III and IV, but not Groups V or VI. Alloxan-induced diabetic rats were fasted for 6 h before the beginning of the experimental procedure. They were divided into control (Group V) and treated groups (Group VI), each of 6 rats. As 250 µL of the extract (equivalent to 300 mg kg −1 ) was administered orally to alloxan treated-group, while 250 µL of the vehicle was administrated orally to the animals of control group. Serum samples were collected at 0, 1, 2, 3, 4 and 6 h following the treatment and were used for glucose and insulin determination. Results: Oral, but not intraperitonial (i.p.) administration of ethanol extract (300 mg kg −1 ) resulted in acute, significant (p<0.05) and time-dependent changes in rat serum glucose and insulin levels in both normal and alloxan-induced diabetic rats. In extract-treated alloxan-free rats, a maximum of 32.9% reduction in serum glucose levels was observed following Intraperitonial Glucose Tolerance Test (IPGTT) using glucose-oxidase assay. A maximum reciprocal increase of 59.5% in serum insulin levels following IPGTT was determined by ELISA. Further, in alloxan/ethanol extract-treated group, a maximum of 31% reduction in serum glucose levels as well as 370.2% increase in serum insulin levels following IPGTT were observed. Conclusion: These results demonstrated that oral administration of the ethanol extract of the dried seedless pulp of Citrullus colocynthis had normo-hypoglycemic (i.e., in normal rats), antihyperglycemic as well as insulinotropic actions in alloxan-induced diabetic rats.

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“…About 2.5-3% of the world's population suffers from this disease, a portion which in some countries reaches 7% or more. 1) Hyperglycemia is an important factor in the development and progression of the complications of diabetes mellitus. 2) Diabetes mellitus has been shown to be a state of increased free radical formation.…”

mentioning

confidence: 99%

Effects of Chard (Beta vulgarisL. var cicla) on the Liver of the Diabetic Rats: A Morphological and Biochemical Study

Saçan

Karabulut-Bulan

Bolkent

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Oxidative stress parameters in type I, type II and insulin-treated type 2 diabetes mellitus; insulin treatment efficiency

Cited by 176 publications

References 29 publications

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

In vivo, Acute, Normo-hypoglycemic, Antihyperglycemic, Insulinotropic Actions of Orally Administered Ethanol Extract of Citrullus colocynthis (L.) Schrab Pulp

Effects of Chard (Beta vulgarisL. var cicla) on the Liver of the Diabetic Rats: A Morphological and Biochemical Study

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