Oxidative stress parameters as biomarkers of risk factor for diabetic foot among the patients with type 2 diabetes

Oliveira, Ana Carla Pozzi; Teixeira, Caio Jordão; Stefanello, Talitha Fernandes; Carrara, Márcia Aparecida; Bazotte, Roberto Barbosa; Sá‐Nakanishi, Anacharis Babeto de; Comar, Jurandir Fernando; Batista, Márcia Regina

doi:10.1590/s1516-89132013005000013

Cited by 5 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, several hypotheses have been emitted in order to understand the origin of the complications observed in diabetic patients. These hypotheses include mitochondria damage, mitochondrial defect in oxidative phosphorylation, increased formation of advanced glycation end products (AGES), increased activity of the polyol pathway, hypoxia, alteration of lipoprotein metabolism, increased protein kinase C activity, alteration of growth factors and cytokine, activities and increased oxidative and reductive stress [15, 22]. Although oxidative stress appears as one of the metabolic events associated to diabetes and its complications the precise mechanisms by which oxidative stress may accelerate the development these complications are still not well understood [7].…”

Section: Discussionmentioning

confidence: 99%

“…To improve our knowledge on the pathogenesis of some metabolic diseases, several clinical and experimental studies have been undertaken in order to understand the role of the oxidative stress in the pathogenesis of diabetes [3, 5]. These investigations have described that free radicals generated during this oxidative process is involved in genesis of diabetes, plays significant role in its development of diabetes and complications [1, 4, 7, 12–15]. In such a context, it appears important to evaluate the level of oxidative stress parameters in blood as biomarkers that can be used to identify and prevent the risk of promoting diabetic complications.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in African patients with type 2 diabetes

et al. 2017

View full text Add to dashboard Cite

Background and purposeStudies demonstrate that free radicals are involved in the pathogenesis of diabetic complications. The aim of this study was to determine the implication of total antioxidant capacity (TAC) and some enzymatic and non-enzymatic antioxidants as suitable biomarkers of diabetic complications risk factors.MethodsA total of 90 patients (70 patients with or without diabetic complications +20 normal healthy) were examined by evaluating the level of lipid peroxidation, nitrogen monoxide (NO), fasting blood glucose, glycated haemoglobin (HbA1c), enzymatic and non-enzymatic antioxidants using standard spectrophotometric methods.ResultsThe fasting blood glucose and HbA1c levels were respectively 2.05 and 2.32 times higher in the group of patients with diabetes and complications (DPWC) compared to those of healthy persons. A statistically higher level of malondialdehyde (MDA), NO and TAC was observed in a group of patients with diabetes and complications compared to those without complications (DPNC). A significant positive correlation was found between catalase (CAT) and fasting blood glucose while a significant and negative correlation was noted between reduced glutathione (GSH) and fasting blood glucose. Also was noted a significant relationship between HbA1c and other markers of oxidative stress.ConclusionsThe results suggest that the plasma levels of CAT, TAC and reduced glutathione could give information on the risk of developing complications of diabetes, considering that the modification of these biomarkers levels were associated with oxidative stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in African patients with type 2 diabetes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Type 2 diabetic patients have been shown to have reduced antioxidant capacity (Kasznicki et al, 2012;Oliveira et al, 2014;Rani, Mynthili, 2014), and, therefore, the effect of oral GDP treatment on oxidative stress was evaluated. Treatment with GDP increased (P=0.004) protein free-thiol groups from 5.99 ± 0.17 nmol/mg protein to 6.73 ± 0.23 nmol/mg protein (M ± SE).…”

Section: Resultsmentioning

confidence: 99%

Glutamine dipeptide supplementation improves clinical responses in patients with diabetic foot syndrome

Krupek

Silva

́Ana

et al. 2016

Braz. J. Pharm. Sci.

Self Cite

View full text Add to dashboard Cite

The effect of glutamine dipeptide (GDP) supplementation in patients with diabetic foot syndrome was evaluated. A total of 22 patients took part in the study. GDP was supplied in 10 g sachets, and was dissolved in water immediately before use, with ingestion once a day, after lunch or after dinner (20 g/day) over a period of 30 days. Quantification of foot insensitive areas, oxidative stress, blood cytokines, and biochemical, hematological and toxicological parameters was performed before and after GDP supplementation. We observed an increase in blood levels of interferon-α (P=0.023), interferon-γ (P=0.038), interleukin-4 (P=0.003), interleukin-6 (P=0.0025), interleukin-7 (P=0.028), interleukin-12 p40 (P=0.017), interleukin-13 (P=0.001), leukocytes (P=0.037), eosinophils (P=0.049), and typical lymphocytes (P<0.001) due to GDP administration. In addition, we observed a reduced number (P=0.048) of insensitive areas on the foot, and reduction (P=0.047) of fasting hyperglycemia. Patients also showed increased blood high density lipoprotein (P<0.01) and protein thiol groups (P=0.004). These favorable results were associated with the absence of renal and hepatic toxicity. These results are of clinical relevance, since supplementation with GDP over 30 days improved clinical responses in patients with diabetic foot syndrome.Uniterms: Type 2 diabetes mellitus/treatment/study. Glutamine/effects. Nutraceuticals.

show abstract

“…Overt induction of oxidative stress has been widely implicated in impaired wound healing and the development of DFU. Indeed, alteration in the oxidative stress status was associated with loss of foot sensitivity and the development of ulcerations in DFU patients 27 . Oxidative stress is also seen to interact with and exacerbate other stress mediators, including ER stress and inflammation 28 .…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of miR‐146a is associated with exacerbated inflammation, oxidative and endoplasmic reticulum stress in the progression of diabetic foot ulcer

Vikraman,

Amin,

Mohandas

et al. 2024

Wound Repair Regeneration

View full text Add to dashboard Cite

Recent evidence has implicated the role of microRNA‐146a (miR‐146a) in regulating inflammatory responses. In the present study, we investigated the role of miRNA‐146a in the progression of diabetic foot ulcer (DFU) in type 2 diabetes mellitus patients (T2DM) and studied its correlation with stress mediators such as Endoplasmic Reticulum (ER) and oxidative stress. Ninety subjects were enrolled and evenly distributed among three groups: Controls (n = 30), T2DM without complications (n = 30) and T2DM with foot ulcers (n = 30). Subsequently, each group was further subdivided based on the University of Texas classification. Peripheral blood was collected from all the study subjects, while tissue biopsies were taken only from DFU patients. Total RNA from both PBMCs and wound tissues were isolated using miRNA isolation kit and qPCR was performed to check the expression of miR‐146a, ER stress and oxidative stress markers. Our findings revealed a significant decrease in miR‐146a expression among T2DM patients with Grade 2 and Grade 3 DFUs compared with those with Grade 0 and Grade 1 DFUs. Notably, inflammatory genes regulated by miR‐146a, including TRAF6, IRAK‐1 and ADAM, were all upregulated in T2DM patients with Grade 2 and Grade 3 DFUs. Moreover, reduced miR‐146a levels were correlated with increased markers of ER stress and oxidative stress in Grade 2 and Grade 3 DFU patients. Furthermore, our in vitro experiment using mouse 3T3 fibroblasts demonstrated a downregulation of miR‐146a following induction of hyperglycaemia, ER stress and oxidative stress in these cells. These findings suggest a potential link between diminished miR‐146a expression and heightened oxidative and ER stress in T2DM patients with more severe grades of DFUs. Our results imply that targeting miR‐146a may hold therapeutic promise for managing disease progression in DFU patients, as it could help alleviate oxidative and ER stress associated with diabetic complications.

show abstract

Oxidative stress parameters as biomarkers of risk factor for diabetic foot among the patients with type 2 diabetes

Abstract: ABSTRACT

Cited by 5 publications

References 19 publications

Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in African patients with type 2 diabetes

Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in African patients with type 2 diabetes

Glutamine dipeptide supplementation improves clinical responses in patients with diabetic foot syndrome

Dysregulation of miR‐146a is associated with exacerbated inflammation, oxidative and endoplasmic reticulum stress in the progression of diabetic foot ulcer

Contact Info

Product

Resources

About