Oxidative stress parameters and erythrocyte membrane adenosine triphosphatase activities in streptozotocin-induced diabetic rats administered aqueous preparation ofKalanchoe Pinnataleaves

Abstract

Background:Diabetes mellitus is a chronic metabolic disease that according to the World Health Organization affects more than 382 million people. The rise in diabetes mellitus coupled with the lack of an effective treatment has led many to investigate medicinal plants to identify a viable alternative.Objective:To evaluate red blood cell (RBC) membrane adenosine triphosphatase (ATPase) activities and antioxidant levels in streptozotocin-induced diabetic rats administered aqueous preparation of Kalanchoe pinnata leaves.Materials and Methods:Diabetes mellitus was induced in rats by a single administration of streptozotocin (60 mg/kg). Diabetic rats were then treated with aqueous K. pinnata preparation (three mature leaves ~ 9.96 g/70 kg body weight or about 0.14 g/kg body weight/day) for 30 days. Serum glucose, RBC membrane ATPase activities, and antioxidant levels were determined.Results:We noted weight loss and reduced food consumption in the treated diabetic group. Serum glucose levels were reduced in the treated diabetic group compared to the other groups. Superoxide dismutase activity and glutathione levels were not significantly elevated in the treated group compared to the diabetic group. However, serum catalase activity was significantly (P < 0.05) increased in the treated diabetic group compared to the other groups. Serum thiobarbituric acid reactive substances were not significantly altered among the groups. There was a significant (P < 0.05) increase in Mg2+ ATPase activity and a nonsignificant increase in Na+/K+ ATPase activity in the RBC membrane of the treated diabetic group compared to the diabetic group.Conclusion:The consumption of aqueous preparation of K. pinnata may accrue benefits in the management of diabetes by lowering oxidative stress often associated with the disease and improving the availability of cellular magnesium through an increase in the magnesium ATPase pump in the RBC membrane for increased cellular metabolism of glucose through the glycolytic pathway.SUMMARY We noted weight loss and reduced food consumption in the diabetic rats treated with K. pinnata preparationSerum glucose levels were reduced in diabetic rats treated with K. pinnata preparationSerum catalase activity was significantly (P < 0.05) increased in diabetic rats treated with K. pinnata preparationWe also noted a significant (P < 0.05) increase in Mg2+ ATPase activity in the RBC membranes of diabetic rats treated with K. pinnata preparationOverall, the consumption of aqueous preparation of K. pinnata lowered oxidative stress often associated with diabetes and improved availability of cellular magnesium through an increase in magnesium ATPase pump in the RBC membrane.

Antihyperglycemic activity of the leaves fromAnnona cherimolamiller and rutin on alloxan-induced diabetic rats

“…Diabetes mellitus (DM) is a heterogeneous metabolic disorder that is characterized by high levels of blood glucose with disturbances of carbohydrate, lipid, and protein metabolism resulting from defects in insulin secretion, insulin action, or both. [ 1 2 3 4 5 6 ] DM affects more than 371 million people worldwide and accounts for >4.8 million deaths each year. [ 7 8 ] In the case of México, the estimates indicates that the number of diabetic patients will increase from >2 million in 2002 to >132 million in 2030.…”

Bioactive Ingredients in K. pinnata Extract and Synergistic Effects of Combined K. pinnata and Metformin Preparations on Antioxidant Activities in Diabetic and Non-Diabetic Skeletal Muscle Cells

“…However, significant increases in catalase activity for all cell lines occurred when cells were treated with 400 μg/mL of K. pinnata . This study suggests that 400 μg/mL of K. pinnata may reduce oxidative damage by increasing catalase activity in human skeletal muscle cells compared to other treatment groups and may be beneficial in reducing oxidative stress in type II diabetes [ 40 ]. The increase in catalase activity in the K. pinnata -only treatment may be associated with the combined action of the bioactive compounds in the extract, as similarly reported in another study [ 10 ].…”

Ciência da Saúde na Amazônia Ocidental

“…A administração do extrato aquoso de K. pinnata demonstrou uma diminuição no peso corporal, e melhorou a atividade hiperglicêmica. A diminuição da glicose no sangue pode ser um fator de redução de peroxidação lipídica e melhoria do perfil antioxidante (MENON et. al., 2016).…”