Oxidative stress mediates drug-induced hepatotoxicity in rats: a possible role of DNA fragmentation

Amin, Amr; Hamza, Alaaeldin Ahmed

doi:10.1016/j.tox.2004.11.039

Cited by 109 publications

(67 citation statements)

References 31 publications

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“…It has been shown that KET can cause hepatotoxicity and testicular toxicity which was revealed by histopathology and biochemistry results (Amin and Hamza, 2005;Amin, 2008). In rats, KET-induced testicular damage was demonstrated to be associated with alterations to testicular levels of malondialdehyde, glutathaione, catalase, and superoxide dismutase (Amin, 2008).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats*

Iamsaard

Burawat

Kanla

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:Background: Ketoconazole (KET), an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria ternatea (CT) are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported. Objective: To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET. Methods: The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Male rats were treated with CT flower extracts (10, 50, or 100 mg/kg BW) or distilled water via a gastric tube for 28 d (preventive period: Days 1-21) and induced by KET (100 mg/kg BW) via intraperitoneal injection for 7 d (induction period: Days 22-28). After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting. Results: The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT+KET groups, CT flower extracts (50 and 100 mg/kg BW) alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100+KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups. Conclusions: C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats.

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Section: Discussionmentioning

confidence: 99%

Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats*

Iamsaard

Burawat

Kanla

et al. 2014

J. Zhejiang Univ. Sci. B

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“…Clinical diagnosis of disease and damage to the structural integrity of liver is commonly assessed by monitoring the status of serum SGOT and SGPT activities (Amin and Hamza, 2005). In fact, enzymatic activities of SGOT and SGPT are considered as sensitive serological indicators of liver toxicity.…”

Section: Discussionmentioning

confidence: 99%

Regulation of oxidative stress and lipid peroxidation in PTU induced mice, treated with Pyrroloquinoline quinone (PQQ)

Kumar¹

2013

IOSR-JPBS

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“…Clinical diagnosis of disease and damage to the structural integrity of the liver is commonly assessed by monitoring the status of AST and ALT activities, which are sensitive serum indicators of liver integrity (24). Table 5 shows that treatment with Eugenia caryophyllus at 100 or 200 mg/kg of body weight significantly reduced the levels of AST and ALT.…”

Section: Discussionmentioning

confidence: 99%

Eugenia caryophyllus Extract Exerts Hypocholesterolemic and Antioxidant Effects in High-Cholesterol-Fed Rats

Nwozo¹,

Kasumu²,

Oyinloye³

2015

Avicenna J Med Biochem

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Background: Eugenia caryophyllus (clove) is an important aromatic household spice. The plant is believed to possess medicinal properties and is commonly used in herbal preparations by traditional healers in the treatment of different ailments and diseases. Objectives: We investigated the lipid-lowering and antioxidant effects of aqueous extract of Eugenia caryophyllus on high-cholesterol-fed rats. Materials and Methods: Cholesterol (40 mg/0.3 mL) was administered to induce hypercholesterolemia in rats by oral gavage, and Eugenia caryophyllus (100 or 200 mg/kg) and Questran (0.26 g/kg) were administered five times a week for eight consecutive weeks. Serum lipid-profiles, lipid peroxidation (LPO), and antioxidant parameters were examined in liver and heart post mitochondrial fraction (PMF). Aspartate and alanine aminotransferase (ALT and AST) activities and liver tissue histology were used to evaluate tissue toxicity. Results: Hypercholesterolemia produced a noticeable decrease in serum HDL-c, whereas a concurrent elevation in serum LDL-c, total cholesterol, and triglycerides as well as serum ALT and AST was observed. Furthermore, hypercholesterolemia remarkably decreased antioxidant status, but LPO content was increased. These indices were significantly attenuated in hypercholesterolemic rats treated with E. caryophyllus extract (100 or 200 mg/kg of body weight). Specifically, reduced glutathione (GSH) concentration was increased in a dose dependent manner in liver and heart PMF.Conclusions: These results demonstrate that the hypolipidemic and antioxidative effects of aqueous extract of E. caryophyllus might be due to its ability to ameliorate lipid profiles, enhance antioxidant activities, and delay the lipid peroxidation process. This confirms the previously identified protective roles of E. caryophyllus in human health.

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Oxidative stress mediates drug-induced hepatotoxicity in rats: a possible role of DNA fragmentation

Cited by 109 publications

References 31 publications

Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats*

Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats*

Regulation of oxidative stress and lipid peroxidation in PTU induced mice, treated with Pyrroloquinoline quinone (PQQ)

Eugenia caryophyllus Extract Exerts Hypocholesterolemic and Antioxidant Effects in High-Cholesterol-Fed Rats

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