Abstract:Background: Ketoconazole (KET), an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria ternatea (CT) are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported. Objective: To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET. Methods: The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Male rats were treated with CT flower extracts (10, 50, or 100 mg/kg BW) or distilled water via a gastric tube for 28 d (preventive period: Days 1-21) and induced by KET (100 mg/kg BW) via intraperitoneal injection for 7 d (induction period: Days 22-28). After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting. Results: The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT+KET groups, CT flower extracts (50 and 100 mg/kg BW) alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100+KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups. Conclusions: C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats.
Objective. is study aimed to investigate the sensitivity of the testis, epididymis, seminal vesicle, and sperm acrosome reaction (AR) to monosodium L-glutamate (MSG) in rats. Materials and methods. Rats were divided into four groups and fed with non-acidic MSG at 0.25, 3 or 6 g/kg body weight for 30 days or without MSG. e morphological changes in the reproductive organs were studied. e plasma testosterone level, epididymal sperm concentration, and sperm AR status were assayed. Results. Compared to the control, no signi cant changes were discerned in the morphology and weight of the testes, or the histological structures of epididymis, vas deferens and seminal vesicle. In contrast, signi cant decreases were detected in the weight of the epididymis, testosterone levels, and sperm concentration of rats treated with 6 g/kg body weight of MSG. e weight loss was evident in the seminal vesicle in MSG-administered rats. Moreover, rats treated with MSG 3 and 6 g/kg exhibited partial testicular damage, characterized by sloughing of spermatogenic cells into the seminiferous tubular lumen, and their plasma testosterone levels were signicantly decreased. In the 6 g/kg MSG group, the sperm concentration was signi cantly decreased compared with the control or two lower dose MSG groups. In AR assays, there was no statistically signi cant di erence between MSG-rats and normal rats. Conclusion. Testicular morphological changes, testosterone level, and sperm concentration were sensitive to high doses of MSG while the rate of AR was not affected. erefore, the consumption of high dose MSG must be avoided because it may cause partial infertility in male.
SUMMARY:Spermatogenesis is a major process in testis occurring from puberty through life span of males. The tyrosine phosphorylation is assumed to play roles in spermatogenesis because this process is important for cell proliferations, divisions, and differentiations. However, the localizations and identifications of phosphorylated proteins in testicular tissue of adult male rats are still unclear. Therefore, this study attempted to immuno-localize and identify such proteins in testicular tissues of Sprague-Dawley rats. The monoclonal anti-phosphotyrosine (clone 4G10) was used to probe tyrosine phosphorylated proteins and also to examine the expression of such proteins using immuno-Western blotting in rat testis. The result showed that positive reactivity of tyrosine phosphorylated proteins was clearly observed in interstitial endocrine cells (Leydig cells), sustentocytes (Sertoli cells), spermatogonia, spermatocytes, and spermatids (round and elongated), respectively. The expressions of testicular tyrosine phosphorylated proteins were 200, 131, 93, 70, 60, and 48 kDas, respectively. In conclusion, testicular tyrosine phosphorylated proteins were localized in both germinal epithelium and interstitial endocrine cells of adult Sprague-Dawley rats.
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