Oxidative stress markers in preovulatory follicular fluid in humans

Jóźwik, Marcin; Wołczyński, Sławomir; Szamatowicz, M

doi:10.1093/molehr/5.5.409

Cited by 149 publications

(116 citation statements)

References 20 publications

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“…Very low G6PD activity was found in the follicular liquid, which could be coming from dead cells or erythrocytes, since at this time the follicles are more vascularized. On its behalf, GSH could be playing an important antioxidant role in the follicular liquid, since it is responsible for safeguarding the integrity and viability not only of the oocyte but also of the follicular cells (Jozwik et al 1999). The decrease in GSH level in the follicular liquid could explain the increase in proteins modified by free radicals in the atretic follicles (Fig.…”

Section: Discussionmentioning

confidence: 99%

Changes in the glucose-6-phosphate dehydrogenase activity in granulosa cells during follicular atresia in ewes

et al. 2009

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Apoptosis of granulosa cells during follicular atresia is preceded by oxidative stress, partly due to a drop in the antioxidant glutathione (GSH). Under oxidative stress, GSH regeneration is dependent on the adequate supply of NADPH by glucose-6-phosphate dehydrogenase (G6PD). In this study, we analyzed the changes of G6PD, GSH, and oxidative stress of granulosa cells and follicular liquid and its association with apoptosis during atresia of small (4-6 mm) and large (O6 mm) sheep antral follicles. G6PD activity was found to be higher in granulosa cells of healthy small rather than large follicles, with similar GSH concentration in both cases. During atresia, increased apoptosis and protein oxidation, as well as a drop in GSH levels, were observed in follicles of both sizes. Furthermore, the activity of G6PD decreased in atretic small follicles, but not in large ones. GSH decreased and protein oxidation increased in follicular fluid. This was dependent on the degree of atresia, whereas the changes in G6PD activity were based on the type of follicle. The higher G6PD activity in the small follicles could be related to granulosa cell proliferation, follicular growth, and a lower sensitivity to oxidative stress when compared with large follicles. The results also indicate that GSH concentration in atretic follicles depends on other factors in addition to G6PD, such as de novo synthesis or activity of other NADPH-producing enzymes. Finally, lower G6PD activity in large follicles indicating a higher susceptibility to oxidative stress associated to apoptosis progression in follicle atresia.

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Section: Discussionmentioning

confidence: 99%

Changes in the glucose-6-phosphate dehydrogenase activity in granulosa cells during follicular atresia in ewes

et al. 2009

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“…This may provide an explanation for impaired folliculogenesis in association with smoking (107). However, the developmental competence of oocytes retrieved during IVF is a multifactorial process that cannot be attributed to OS alone (108).…”

Section: T a B L Ementioning

confidence: 98%

Role of reactive oxygen species in the pathophysiology of human reproduction

Agarwal

Saleh

Bedaiwy

2003

Fertility and Sterility

1,276

909

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Objective: To summarize the role of reactive oxygen species (ROS) in the pathophysiology of human reproduction. Design: Review of literature. Setting: Fertility research center and obstetrics and gynecology department in a tertiary care facility. Result(s): ROS plays an essential role in the pathogenesis of many reproductive processes. In male-factor infertility, oxidative stress attacks the fluidity of the sperm plasma membrane and the integrity of DNA in the sperm nucleus. Reactive oxygen species induced DNA damage may accelerate the process of germ cell apoptosis, leading to the decline in sperm counts associated with male infertility. ROS mediated female fertility disorders share many pathogenic similarities with the ones on the male side. These similarities include a potential role in the pathophysiology of endometriosis and unexplained infertility. High follicular fluid ROS levels are associated with negative IVF outcomes, particularly in smokers. Moreover, oxidative stress may be responsible in hydrosalpingeal fluid mediated embryotoxicity as well as poor in vitro embryonic development. Conclusion(s):High levels of ROS are detrimental to the fertility potential both in natural and assisted conception states. (Fertil Steril 2003;79:829 -43.

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“…These results suggest that antioxidant FF HDL particle components may impact embryo quality during IVF. The presence of both oxidative stress markers [42] and antioxidant molecules and enzymes, especially lipophilic micronutrients [43,44], has been reported in developing human ovarian follicles, implying a dynamic biochemical environment. In our recent pilot work, we measured lipophilic micronutrients in FF and demonstrated their associations with embryo quality indicators [22], supporting a potential antioxidant role in the microenvironment of developing oocytes during IVF.…”

Section: Geometric Mean Concentration (µMol/l) Hdl-c Subfractions (Nm)mentioning

confidence: 99%

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Kim

Bloom

Browne

et al. 2016

J Assist Reprod Genet

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Purpose Follicular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF. Methods Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis. Concentrations of 15 non-cholesterol HDL particle components and 26 HDL-cholesterol (HDL-C) particle size subfractions were determined. Embryo quality was assessed for embryo cell number, embryo fragmentation, and embryo symmetry. Multivariable Poisson regression with a sandwich variance estimator was used to evaluate associations between HDL particle components and embryo quality, adjusted for covariates. Results Higher γ-tocopherol concentration was associated with less embryo fragmentation (relative risk [RR] = 4.43; 95 % confidence interval [CI] 1.78, 11.06), and higher apolipoprotein A-1 concentration was associated with full embryo symmetry (RR = 3.92; 95 % CI 1.56, 9.90). Higher concentrations of HDL-C subfractions in the large and medium particle size ranges were associated with poorer embryo quality. Conclusions FF HDL lipophilic micronutrients and protein components, as well as HDL-C particle size, may be important predictors of embryo quality during IVF.

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Oxidative stress markers in preovulatory follicular fluid in humans

Cited by 149 publications

References 20 publications

Changes in the glucose-6-phosphate dehydrogenase activity in granulosa cells during follicular atresia in ewes

Changes in the glucose-6-phosphate dehydrogenase activity in granulosa cells during follicular atresia in ewes

Role of reactive oxygen species in the pathophysiology of human reproduction

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

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