Oxidative stress markers in canine atopic dermatitis

Kapun, A. Plevnik; Salobir, Janez; Levart, Alenka; Kotnik, Tina; Svete, Alenka Nemec

doi:10.1016/j.rvsc.2011.04.014

Cited by 38 publications

(43 citation statements)

References 14 publications

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“…The use of different combinations of superoxide radical generators and detectors may result in non-comparable results among studies. Our results on SOD are in agreement with the results of the study by Plevnik Kapun et al [26] in which the same assay kit and sample type were used. Surprisingly, our results differ greatly from the results of Britti et al [49], although the same assay kit and sample type were used in both studies.…”

Section: Discussionsupporting

confidence: 92%

“…Many modifi cations of the coupled assay have been published, and they vary in substrate concentrations, type of peroxide, presence of chelators and inhibitors, pH, and temperature [15]. The results of our study are in general agreement with the results of studies carried out by Plevnik Kapun et al [26], Winter et al [43] and Stowe et al [44], but are not comparable to others [37,51,52]. In all these studies, the activity of GSH-Px was determined in whole blood using assays based on the method of Paglia and Valentine [9].…”

Section: Discussionsupporting

confidence: 91%

“…However, Rubio et al [36] recently published the results of full validation of an automated assay for the measurement of cupric reducing antioxidant capacity in the serum of dogs. Our results on TAC are in good agreement with the results of studies that employed the same or similar assay kit for the assessment of plasma or serum TAC in healthy dogs [7,26,37,38].…”

Section: Discussionsupporting

confidence: 87%

“…The Ransel kit is based on the method of Paglia and Valentine [9] and has been previously used in dogs [26][27][28]. The Ransod kit is based on the method of McCord and Fridovich [10] and has been previously used in dogs [26,27]. Activities of GSH-Px and SOD were expressed as units per gram of haemoglobin (U/g Hgb).…”

Section: Blood Samples Collection and Analysesmentioning

confidence: 99%

See 3 more Smart Citations

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

et al. 2016

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Data on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No signifi cant difference in age, weight, and antioxidant parameters was determined between females and males. A signifi cant positive effect of age (p = 0.002, r² = 0.284) on superoxide dismutase activity was confi rmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r² = 0.118), as well as age (p = 0.073, r² = 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Blood Samples Collection and Analysesmentioning

confidence: 99%

See 2 more Smart Citations

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

et al. 2016

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“…In children with AD, the urinary concentration of acrolein (CH2ϭCH-CHO), which is a lipid peroxidation product with toxic activity, is markedly higher than in normal children (6,16). Kapun et al also suggested that significantly elevated plasma malondialdehyde levels were found in canine AD patients (17). These findings indicate that oxidative stress, with increased lipid peroxidation, is involved in the pathophysiology of AD.…”

Section: Discussionmentioning

confidence: 99%

Vitamin E Improves Biochemical Indices Associated with Symptoms of Atopic Dermatitis-Like Inflammation in NC/Nga Mice

Hayashi

Sugaya

Ohkoshi

et al. 2012

J Nutr Sci Vitaminol

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Summary We aimed to define whether vitamin E improves biochemical indices associated with symptoms of atopic dermatitis-like inflammation in NC/Nga mice. After picryl chloride (PC) application to their backs, changes in the content of thiobarbituric acid reactive substances (TBARS) and vitamin E, as well as the activity of antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase) were analyzed in the serum and skin of NC/Nga mice during a symptomatic cycle. The levels of inflammatory factors were also assessed, including IgE, cyclooxigenase-2 (COX-2), tumor necrosis factor (TNF-␣ ) and nuclear factor-B (NF-B). When allergic dermatitis was induced by the application of PC to the skin of the mice, skin inflammation appeared 2 wk after PC application, with the peak severity of inflammation observed 5 wk after PC application. Subsequently, the animals recovered from the inflammation by 9 wk after PC application. The TBARS content in the skin and serum increased markedly when the symptoms were the most severe, and decreased to levels near those in control mice by 9 wk after PC application. The activities of SOD and GSHPx in the skin and serum were also positively correlated with symptomatic changes; however, no change in catalase activity was observed 5 wk after PC application. Conversely, vitamin E content decreased at the stage of peak severity. The levels of all inflammatory factors analyzed in this study were altered in a manner similar to other indices. Additionally, vitamin E treatment markedly inhibited these PC-induced alterations. On the basis of these results, it is expected that the observed alterations in biochemical indices, which reflect the symptomatic cycle, may be applicable to objective diagnosis and treatment for atopic dermatitis, and that vitamin E may improve the symptoms of AD.

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Studies on the association of malondialdehyde as a biomarker for oxidative stress and degree of malignancy in dogs with mammary adenocarcinomas

Schroers,

Walter,

Fischer

et al. 2024

Veterinary Medicine & Sci

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BackgroundMammary adenocarcinomas are one of the most common tumour diseases in bitches. The relationship between oxidative stress and the degree of malignancy of the tumour has not been sufficiently researched in veterinary medicine.ObjectivesThe main objective was to investigate the potential role of MDA as a practice‐relevant biomarker for the assessment of systemic oxidative stress and to determine whether this parameter can indicate the malignancy grade of a mammary adenocarcinoma.MethodsIn the present pilot study, MDA plasma concentrations were analysed in 55 bitches with (n = 28) and without (n027) malignant adenocarcinomas of the mammary gland using two different measurement methods and the relationship to tumour size was investigated.ResultsThe mean MDA concentration measured by enzyme‐linked immunosorbent assay (ELISA) was 289 ng/mL (range 365–634 ng/mL) in dogs with grade 1 adenocarcinoma (n = 13), 288.5 ng/mL (range 85–752 ng/mL) in dogs with grade 2 adenocarcinoma (n = 10), 332 ng/mL (range 239–947 ng/mL) in dogs with grade 3 (n = 5) adenocarcinoma and 293 ng/mL (range 175–549 ng/mL) in dogs without a mammary tumour (n = 27). When MDA was measured by HPLC, the average MDA concentration in the study group (n = 11) was 0.24 µmol/L (range 0.16–0.37) and that of the control group (n = 15) was 0.27 µmol/L (range 0.16–1.62). Thus, there were no significant differences between the study group with malignant adenocarcinomas and the control group in both examination methods (p > 0.05). Furthermore, there was no correlation between the MDA concentrations and the approximate volume of the mammary tumour.ConclusionThe results highlight the challenges of providing a prognosis for the malignancy of a mammary adenocarcinoma based on MDA concentrations in plasma using ELISA or HPLC. As a result, histopathological examination remains the gold standard for diagnosing and differentiating adenocarcinomas of the mammary gland.

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Oxidative stress markers in canine atopic dermatitis

Cited by 38 publications

References 14 publications

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

Vitamin E Improves Biochemical Indices Associated with Symptoms of Atopic Dermatitis-Like Inflammation in NC/Nga Mice

Studies on the association of malondialdehyde as a biomarker for oxidative stress and degree of malignancy in dogs with mammary adenocarcinomas

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