Oxidative stress, l-arginine–nitric oxide and arginase pathways in platelets from adolescents with anorexia nervosa

Pereira, Natália Rodrigues; Moss, Monique Bandeira; Assumpção, Carmen Regina Leal de; Cardoso, Catarina; Mann, Giovanni E.; Brunini, Tatiana M.C.; Mendes-Ribeiro, Antônio Cláudio

doi:10.1016/j.bcmd.2009.12.003

Cited by 22 publications

(11 citation statements)

References 34 publications

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“…Proteins were quantified using the BCA protein assay reagent (Pierce, Rockford, IL, USA). Samples containing 20 μg protein were prepared in equal volumes of sample application buffer (NuPage LDS Sample Buffer; Invitrogen, Carlsbad, CA, USA), separated by 10% sodium dodecyl sulphate–polyacrylamide gel electrophoresis (Invitrogen), transferred to polyvinylidene difluoride membranes and then immunoblotted with mouse monoclonal antibodies against human endothelial (e) and inducible (i) NOS (BD Transduction, San Jose, CA, USA) at a dilution of 1 : 2500 21 . Gels were stained with Coomassie blue to check protein transfer.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, samples from platelets or plasma were mixed with 1 mL of 10% trichloroacetic acid and 1 mL of 0.67% thiobarbituric acid. They were then heated in a boiling water bath for 30 min 21 and TBARS were determined by absorbance at 532 nm. Results are expressed as malondialdehyde (MDA) equivalents (nmol/mg protein).…”

Section: Methodsmentioning

confidence: 99%

“…Platelets and plasma were used to determine superoxide dismutase (SOD) activity, which was assayed by measuring the inhibition of adrenaline auto‐oxidation as absorbance at 480 nm 21 . The total protein content of each sample was determined by the Bradford method, as described previously 21 …”

Section: Methodsmentioning

confidence: 99%

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Platelet activation, oxidative stress and overexpression of inducible nitric oxide synthase in moderate heart failure

Meirelles¹,

Resende²,

Matsuura³

et al. 2011

Clinical and Experimental Pharmacology and Physiology

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1. Chronic heart failure (CHF) is a common disabling disorder associated with thromboembolic events, the genesis of which is not yet fully understood. Nitric oxide (NO), derived from the vascular endothelium and platelets, has an important role in the physiological regulation of blood flow. It is generated from the amino acid L-arginine via NO synthase (NOS). 2. The main objective of the present study was to investigate NO production and its relationship with platelet aggregation, oxidative stress, inflammation and related amino acids in patients with moderate CHF. The expression and activity of NOS isoforms were analysed by western blotting and conversion of L-[(3)H]-arginine to L-[(3)H]-citrulline, respectively, in CHF patients (n = 12) and healthy controls (n = 15). Collagen- and ADP-induced platelet aggregation, oxidative stress (thiobarbituric acid-reactive substances (TBARS) formation and superoxide dismutase (SOD) activity) and plasma levels of amino acids and inflammatory markers (fibrinogen and C-reactive protein (CRP)) were also determined. 3. Both collagen- and ADP-induced platelet aggregation were increased in CHF patients compared with controls. Platelets from CHF patients did not show any changes in NOS activity in the presence of overexpression of inducible NOS. Systemic and intraplatelet TBARS production was elevated, whereas SOD activity was decreased in CHF patients. l-arginine plasma concentrations were lower in CHF patients than in controls. Systemic levels of CRP and fibrinogen were increased in CHF patients. 4. The results show that, in patients with moderate CHF, there is platelet activation and reduced intraplatelet NO bioavailability due to oxidative stress, which suggests a role for platelets in the prothrombotic state.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Platelet activation, oxidative stress and overexpression of inducible nitric oxide synthase in moderate heart failure

Meirelles¹,

Resende²,

Matsuura³

et al. 2011

Clinical and Experimental Pharmacology and Physiology

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“…However, reports in the literature are scarce and contain conflicting data on the pattern of NO behavior in AN. In contrast to the aforementioned studies [1, 15], Rodrigues-Pereira et al [16] demonstrated diminished NO production in patients with AN, as evaluated by NOS activity and cyclic guanosine monophosphate (cGMP) in platelets.…”

Section: Discussionmentioning

confidence: 96%

Elevated levels of exhaled nitric oxide in patients with anorexia nervosa

Oświęcimska

Ziora

et al. 2013

Eur Child Adolesc Psychiatry

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BackgroundNitric oxide (NO) is involved in eating behavior and inflammatory response. Moreover, there is evidence that NO production is altered in patients with anorexia nervosa (AN).AimTo assess whether the overproduction of NO in AN can affect NO level in exhaled air.Materials and methodsExhaled NO level was studied in 23 girls with AN and compared with that of healthy age- and gender-matched nonatopic controls.ResultsExhaled NO levels were significantly higher in girls with AN compared with healthy age-matched controls.ConclusionsIt appears that anorexia nervosa was accompanied by a higher level of exhaled NO, likely resulting from a systemic increase in NO production because of the severe catabolic state.

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“…•-) [6]. Physiological enzymatic antioxidant defense mechanisms such as glutathione peroxidase and catalase are essential to avoid excessive and deleterious oxidative damage to endogenous molecules [7].…”

Section: Introductionmentioning

confidence: 99%

Diminished nitric oxide generation from neutrophils suppresses platelet activation in chronic renal failure

Abrantes¹,

Brunini²,

Matsuura³

et al. 2014

Mol Cell Biochem

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Chronic renal failure (CRF) is a complex clinical condition associated with accelerated atherosclerosis and thrombosis leading to cardiovascular events. The aim of this study was to investigate in detail the NO pathway in neutrophils obtained from hemodialysis patients and its association with platelet function and oxidative status. Fifteen CRF patients on hemodialysis and fifteen controls were included in this study. Laboratory and experimental evaluations were performed after hemodialysis in CRF patients. We evaluated L-[³H] arginine transport, NO synthase (NOS) activity, amino acid concentration in neutrophils, and expressions of NOS isoforms and p47(phox) by western blotting. Platelet aggregation was analyzed in the presence or absence of neutrophils. Oxidative status was measured through glutathione peroxidase, catalase activities, protein oxidation, lipid peroxidation, and DNA/RNA oxidation in serum. Basal NOS activity (pmol/10⁶ cells/min) was impaired in CRF patients on hemodialysis (0.33 ± 0.17) compared to controls (0.65 ± 0.12), whereas the expression of NOS isoforms remained unaltered. L-Arginine transport into neutrophils was similar in CRF patients on hemodialysis and controls. In addition, intracellular concentration of L-arginine was increased fourfold in the patient group. Systemic oxidative stress markers were not affected by CRF. On the other hand, NADPH oxidase subunit p47(phox) in neutrophils was overexpressed in CRF. In the presence of neutrophils, there was a reduction time-dependent in platelet aggregation in both groups with no difference between them. This data suggest that reduced basal generation of NO by neutrophils in CRF patients on hemodialysis occurs independently of L-arginine bioavailability and is able to suppress platelet activation.

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Oxidative stress, l-arginine–nitric oxide and arginase pathways in platelets from adolescents with anorexia nervosa

Cited by 22 publications

References 34 publications

Platelet activation, oxidative stress and overexpression of inducible nitric oxide synthase in moderate heart failure

Platelet activation, oxidative stress and overexpression of inducible nitric oxide synthase in moderate heart failure

Elevated levels of exhaled nitric oxide in patients with anorexia nervosa

Diminished nitric oxide generation from neutrophils suppresses platelet activation in chronic renal failure

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