Oxidative stress is responsible for genotoxicity of camphorquinone in primary human gingival fibroblasts

Wessels, Miriam; Leyhausen, Gabriele; Volk, Joachim; Geurtsen, Werner

doi:10.1007/s00784-013-1178-x

Cited by 18 publications

(16 citation statements)

References 38 publications

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“…Recently, we reported that CQ caused DNA damage in association with an increased ROS generation in primary human gingival fibroblasts [13]. Furthermore, we documented that this damage was in part of oxidative nature [40]. These findings are supported by our present study, which shows an increase of tail moment for CQ concentrations of 2.5 mM and higher.…”

Section: Okf6/tert2 Cellssupporting

confidence: 90%

“…Former studies show a genotoxic potential already at lower milimolar concentrations of CQ in cultures of human gingival fibroblasts [13,40]. Therefore OKF6/TERT2 (as first target cells) may have very efficient and fast repair mechanisms and/or more effective oxidative defense mechanisms thus preventing DNA damage due to higher ROS levels.…”

Section: Cq (A Paired T-test) and Cells Treated With 2mm Cq + Homentioning

confidence: 99%

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Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

et al. 2015

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Section: Okf6/tert2 Cellssupporting

confidence: 90%

Section: Cq (A Paired T-test) and Cells Treated With 2mm Cq + Homentioning

confidence: 99%

Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

et al. 2015

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“…It is conceivable that incorporation of hydrophilic photoinitiators in resin will allow photoinitiators to diffuse more freely within the hydrophilic-rich phases in higher concentrations. Current dentin adhesives should be optimized to include an adequate concentration of efficient hydrophilic photoinitiators, but it must also be considered that photoinitiators in high concentration could elicit cytotoxic effect (Wessels et al 2014). Ongoing research is exploring novel, efficient hydrophilic photoinitiators that will be compatible with resin/water in the oral cavity and capable of imparting optimal polymerization to the hydrophilic-rich phase.…”

Section: Discussionmentioning

confidence: 99%

Polymerization Behavior of Hydrophilic-Rich Phase of Dentin Adhesive

Abedin

Parthasarathy

et al. 2015

J Dent Res

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The 2-fold objectives of this study were 1) to understand whether model hydrophobic-and hydrophilic-rich phase mimics of dentin adhesive polymerize similarly and 2) to determine which factor, the dimethacrylate component, bisphenol A glycerolate dimethacrylate (BisGMA) or photoinitiator concentration, has greater influence on the polymerization of the hydrophilic-rich phase mimic. Current dentin adhesives are sensitive to moisture, as evidenced by nanoleakage in the hybrid layer and phase separation into hydrophobic-and hydrophilic-rich phases. Phase separation leads to limited availability of the cross-linkable dimethacrylate monomer and hydrophobic photoinitiators within the hydrophilic-rich phase. Model hydrophobic-rich phase was prepared as a single-phase solution by adding maximum wt% deuterium oxide (D 2 O) to HEMA/BisGMA neat resins containing 45 wt% 2-hydroxyethyl methacrylate (HEMA). Mimics of the hydrophilicrich phase were prepared similarly but using HEMA/BisGMA neat resins containing 95, 99, 99.5, and 100 wt% HEMA. The hydrophilic-rich mimics were prepared with standard or reduced photoinitiator content. The photoinitiator systems were camphorquinone (CQ)/ethyl 4-(dimethylamino)benzoate (EDMAB) with or without [3-(3, 4-dimethyl-9-oxo-9H-thioxanthen-2-yloxy)-2-hydroxypropyl]trimethylammonium chloride (QTX). The polymerization kinetics was monitored using a Fourier transform infrared spectrophotometer with a time-resolved collection mode. The hydrophobic-rich phase exhibited a significantly higher polymerization rate compared with the hydrophilic-rich phase. Postpolymerization resulting in the secondary rate maxima was observed for the hydrophilic-rich mimic. The hydrophilic-rich mimics with standard photoinitiator concentration but varying cross-linker (BisGMA) content showed postpolymerization and a substantial degree of conversion. In contrast, the corresponding formulations with reduced photoinitiator concentrations exhibited lower polymerization and inhibition/delay of postpolymerization within 2 h. Under conditions relevant to the wet, oral environment, photoinitiator content plays an important role in the polymerization of the hydrophilic-rich phase mimic. Since the hydrophilic-rich phase is primarily water and monomethacrylate monomer (e.g., HEMA as determined previously), substantial polymerization is important to limit the potential toxic response from HEMA leaching into the surrounding tissues.

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“…Finally, camphorquinone increased DNA damage in primary human gingival fibroblasts at all concentrations as a result of intracellular oxidative stress and subsequent down-regulation of glutathione level (52,53).…”

Section: Dental Restorative Materials and Related Compoundsmentioning

confidence: 95%

Genotoxicity Induced by Dental Materials: A Comprehensive Review

2017

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Oxidative stress is responsible for genotoxicity of camphorquinone in primary human gingival fibroblasts

Abstract: A more detailed insight into genotoxic mechanisms in oral cells can be of great importance for a better understanding of the biocompatibility of CQ.

Cited by 18 publications

References 38 publications

Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

Polymerization Behavior of Hydrophilic-Rich Phase of Dentin Adhesive

Genotoxicity Induced by Dental Materials: A Comprehensive Review

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