Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

Wessels, Miriam; Rimkus, Julia; Leyhausen, Gabriele; Volk, Joachim; Geurtsen, Werner

doi:10.1016/j.dental.2015.06.007

Cited by 16 publications

(7 citation statements)

References 55 publications

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“…However, the cytotoxic results from Manojlovic and Van Landuyt showed that TPO was respectively 7 times and 10 times more toxic on human cell line of foetal lung fibroblasts MRC-5 than CQ [364,388]. Furthermore, CQ is responsible for mutagenicity in bacterial umu test as well as genotoxicity in the Comet assay performed on human oral and intestinal cell lines [374,392]. It was shown that CQ induced the generation of intracellular reactive oxygen and nitrogen species (ROS/RNS), causing damage of DNA in cultured primary human gingival fibroblasts [393], apoptosis and/or necrosis [394,395].…”

Section: Short Overview On the Toxicity Of Free Radical-photoinduced ...mentioning

confidence: 99%

Bio-sourced monomers and cationic photopolymerization–The green combination towards eco-friendly and non-toxic materials

Pierau

Elian

Akimoto

et al. 2022

Progress in Polymer Science

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Section: Short Overview On the Toxicity Of Free Radical-photoinduced ...mentioning

confidence: 99%

Bio-sourced monomers and cationic photopolymerization–The green combination towards eco-friendly and non-toxic materials

Pierau

Elian

Akimoto

et al. 2022

Progress in Polymer Science

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“…Not only do the degree of conversion and monomer release determine the biocompatibility of adhesives, but the cytotoxicity of the photoinitiator should also be taken into account, as these reactive compounds are not bound to the matrix and may leach from the adhesives [ 20 ]. Camphorquinone, which is included in Single Bond Universal at millimolar concentrations, induces oxidative DNA damage and generation of reactive oxygen species [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Nowicka

Łagocka

Lipski

et al. 2016

BioMed Research International

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Objective. This study presents a clinical and histological evaluation of human pulp tissue responses after direct capping using a new dentin adhesive system. Methods. Twenty-eight caries-free third molar teeth scheduled for extraction were evaluated. The pulps of 22 teeth were mechanically exposed and randomly assigned to 1 of 2 groups: Single Bond Universal or calcium hydroxide. Another group of 6 teeth acted as the intact control group. The periapical response was assayed, and a clinical examination was performed. The teeth were extracted after 6 weeks, and a histological analysis was performed. The pulp status was assessed, and the thickness of the dentin bridge was measured and categorized using a histological scoring system. Results. The clinical phase was asymptomatic for Single Bond Universal patients. Patients in the calcium hydroxide group reported mild symptoms of pain, although the histological examination revealed that dentin bridges with or without limited pulpitis had begun forming in each tooth. The universal adhesive system exhibited nonsignificantly increased histological signs of pulpitis (P > 0.05) and a significantly weaker thin mineralized tissue layer (P < 0.001) compared with the calcium hydroxide group. Conclusion. The results suggest that Single Bond Universal is inappropriate for human pulp capping; however, further long-term studies are needed to determine the biocompatibility of this agent.

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“…[5,7,[14][15][16]. Dickson et al [1] described a protocol in which this cell line was cultured in KSFM, which has since been widely cited and replicated [17][18][19][20][21]. Dulbecco's Modified Eagle Medium/Nutrient Mixture of Hams F-12 (DMEM/F-12) is another standard basal medium commonly containing 1.05 mM calcium [22].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

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Background: Understanding in vitro expansion of OKF6/TERT-2 oral epithelial cells is important for studying molecular biology of disease and pathology affecting the oral cavity. The media used for any cell culture is paramount in terms of efficient output. Therefore, this study aims to compare two different media for OKF6/TERT-2 cultures: Keratinocyte Serum-Free Medium (KSFM) and a composite medium comprised of DMEM/F-12 mixed with KSFM (referred to as DFK). For application purposes, this investigation also compares the toxicological effects of flavored electronic cigarette liquids (E-liquids) on OKF6/TERT-2 cultures grown in both media. Methods: Cells were grown in KSFM and DFK media and cellular growth, morphology, gene expression of mucins and tight junctions, as well as wound-healing were determined. Additionally, cellular viability and cytotoxicity were indexed after E-liquid exposures. Results: While overall cellular morphologies remained unaltered, cells grown in DFK reached confluency faster. Except for claudin-1, there is no appreciable difference in expression of the other genes tested. Additionally, cultures in DFK appear more sensitive to E-liquids ± flavors. Conclusions: DFK is an alternative medium for cultivation of OKF6/TERT-2 cells to study molecular biology of disease and pathology, such as their responses to E-liquids ± flavors.

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Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

Cited by 16 publications

References 55 publications

Bio-sourced monomers and cationic photopolymerization–The green combination towards eco-friendly and non-toxic materials

Bio-sourced monomers and cationic photopolymerization–The green combination towards eco-friendly and non-toxic materials

Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

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