Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Nowicka, Alicja; Łagocka, Ryta; Lipski, Mariusz; Parafiniuk, Mirosław; Grocholewicz, Katarzyna; Sobolewska, Ewa; Witek, Agnieszka; Buczkowska-Radlińska, Jadwiga

doi:10.1155/2016/2591273

Cited by 26 publications

(23 citation statements)

References 39 publications

(101 reference statements)

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“…There are claims of using dental adhesives in direct contact with the pulp, as the means of direct pulp cupping. In this treatment method, the dental adhesives showed no hard tissue formation induction in comparison to Ca(OH) 2 cements [28][29][30][31]. There was also no expression of type 3 collagen and fibronectin, which were present in teeth treated with Ca(OH) 2 cements [29].…”

Section: Introductionmentioning

confidence: 81%

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

Wawrzynkiewicz

Rozpędek‐Kamińska

Galita

et al. 2020

IJMS

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Dental universal adhesives are considered an useful tool in modern dentistry as they can be used in different etching techniques, allow for simplified protocol and provide sufficient bond strength. However, there is still no consensus as to their toxicity towards pulp. Thus, the present study aimed to evaluate the cytotoxicity and genotoxicity of three universal adhesives: OptiBond Universal, Prime&Bond Universal and Adhese in an in vitro experimental model, monocyte/macrophage cell line SC (ATCC CRL-9855). The cytotoxicity was measured by means of XTT assay, whereas the genotoxicity (comet assay) was evaluated based on the percentage of DNA present in the comet tail. Furthermore, the ability of the adhesives to induce apoptosis was analyzed using flow cytometry (FC) with the FITC annexin V/propidium iodide (PI) double staining. The analysis of the cell cycle progression was performed with FC using PI staining. OptiBond Universal presented significant, while Prime&Bond Universal and Adhese Universal had minimal cytotoxicity and genotoxicity towards human SC cells. Moreover, only OptiBond Universal increased the level of apoptosis in SC cell line. None of the adhesives showed significant cell cycle arrest, as revealed by FC analysis. Due to substantial differences in toxicity in in vitro studies of dental adhesives, there is a great need for further research in order to establish more reliable test protocols allowing for standardized methodology.

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Section: Introductionmentioning

confidence: 81%

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

Wawrzynkiewicz

Rozpędek‐Kamińska

Galita

et al. 2020

IJMS

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“…There were 10 patients participating in this study. Each of them did observe three times (indication, 1 week after treatment, and more than one month after treatment) ( 5 , 31 ). The doctor would check or make sure the parts of teeth that would be filled by restoration as the treatment in the first visit.…”

Section: Resultsmentioning

confidence: 99%

“…In the second visit, the dentist would fill restoration in the cavities, however the fillings were temporary. In the third, it would make restoration the cavities permanently ( 31 , 32 ). In other words, there were 150 images used in this research.…”

Section: Resultsmentioning

confidence: 99%

Dental radiography image enhancement for treatment evaluation through digital image processing

et al. 2018

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BackgroundEvaluation of dental treatment is performed by observing dental periapical radiography to obtain information of filling’s condition, pulp tissue, remain dentin thickness, periodontal ligament, and lamina dura. Nevertheless, the radiographic image used often has low quality due to the level of x-ray radiation made low purposely in order to prevent health problem and limited tools capability. This low quality of the radiographic image, for examples, low image contrast, less brightness, and noise existence cause periapical radiography evaluation hard to be performed. This study aims to improve dental radiographic image quality for assisting pulp capping treatment evaluation.Material and MethodsThe research methodology consists of three main stages, i.e. data collection, image enhancement method production, and result validation. Radiographic image data collection in The Dental Hospital UMY. Image enhancement method has been conducted by comparing several methods: contourlet transform (CT), wavelet transform, contrast stretching (CS), and contrast limited adaptive histogram equalization (CLAHE) to reduce noise, to optimize image contrast, and to enhance image brightness.ResultsThe result of this study is according to mean square error (MSE) and peak signal to noise ratio (PSNR) statistics evaluation, it obtains that the highest scores of MSE and PSNR in row gained from CT method totaled 5.441453 and 40.53652, followed by CLAHE method with the scores are 10.66326 and 38.00736, CS method whose scores are 12.39881 and 39.18518, and the last is wavelet method with the scores are 15.41569 and 36.25343.ConclusionsNonetheless, MSE and PSNR scores are not enough merely to give a recommendation of any suitable methods for improving contrast, therefore, it needs another success parameter coming from the dentist. Key words:Dental radiography, image enhancement, digital image processing.

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“…In 1996, an adhesive system was first adopted for DPC to treat fractured teeth, and a number of studies have been performed to investigate its effectiveness (49). Six RCTs were reviewed that evaluated the effectiveness of an adhesive system for DPC (2,(50)(51)(52)(53)(54); however, the follow-up periods of most of these studies were short. Only one of these studies was included in the present review (2).…”

Section: Adhesive Systemmentioning

confidence: 99%

Long-term clinical and radiographic evaluation of the effectiveness of direct pulp-capping materials

2019

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The aim of this review was to assess the effectiveness of different direct pulp-capping (DPC) materials for human pulp-exposed teeth. An electronic search was performed on 20 February 2018. Long-term clinical and radiographic evaluations of the effectiveness of different DPC materials for use on human pulp-exposed teeth were included. Risk-ofbias assessment and data extraction were performed. From the 496 identified articles, 15 met the eligibility criteria. Among the studies included in those articles, a total of 1,322 teeth were treated with 12 types of DPC materials, and 1,136 teeth were evaluated at a final follow-up examination. For mineral trioxide aggregate (MTA) and calcium hydroxide (CH), the number of included studies, the number of treated teeth, and the mean follow-up period of studies were almost equal, and the success rates of MTA was superior to CH. Therefore, MTA is likely to be a more effective and predictable material for DPC compared to CH. However, the results were based on the included studies, which were all judged to have a high risk of bias. Therefore, more long-term clinical and radiographic studies designed with lower risk of bias are needed. Moreover, the other 10 materials were only investigated by a small number of studies; therefore, further studies are required.

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Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Cited by 26 publications

References 39 publications

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

Dental radiography image enhancement for treatment evaluation through digital image processing

Long-term clinical and radiographic evaluation of the effectiveness of direct pulp-capping materials

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