Oxidative stress induced by gibberellic acid in bone of suckling rats

Troudi, Afef; Amara, Ibtissem Ben; Soudani, Nejla; Bouaziz, Hanen; Boudawara, Tahia; Zeghal, Najiba

doi:10.1016/j.ecoenv.2010.10.010

Cited by 10 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their potential hazardous effects on human health are relatively unexplored (Troudi et al 2010). In the present study, gibberellic acid (GA 3 ) and indoleacetic acid (IAA) were chosen because information on their toxicological biological effects in higher animals is very limited.…”

Section: Discussionmentioning

confidence: 99%

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

2013

View full text Add to dashboard Cite

The aim of the present study was to investigate the protective role of grape seed extract (containing proanthocyandin) against the adverse effects of plant growth regulators (GA 3 (gibberellic acid) and IAA (indoleacetic acid)). The present data showed that the administration of either GA 3 and IAA caused undesirable changes in both hepatic and testicular structure. This was evidenced by a disturbed hepatic strands, pyknotic nuclei, central vein with collapsed endothelium, dilatation in bile sinusoids, congested blood vessel, binucleatd hepatocytes, lymphocytic infiltration, vacuolation, giant hepatic cells, increased Kupffer cells and karyoryxis. Additionally, it was shown that degenerative changes in the testis, spermatogenic arrest, moderate tubular necrosis, Leydig cell degeneration and reduction in the number and size of the seminiferous tubules with some spermatogonia detached from the basement membrane. Concerning flow cytometric study of the liver a significant decrease in G0/1 % and a significant increase in S phase %, G2/ M %, P 53 % and apoptosis % (sub G1) were detected. However, in testis the data recorded a significant decrease in the percentage of mature sperm (percentage of haploid cells) and a significant increase in the percentage of spermatide, diploid cells, P 53 and of apoptotic cells. On the other hand, a distinct recovery of the mentioned hepatic and testicular histopathological and cytogenetic disorders was observed when proanthocyanidin was supplemented to rats administered either of the plant growth hormones (GA 3 and IAA).

show abstract

Section: Discussionmentioning

confidence: 99%

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

2013

View full text Add to dashboard Cite

show abstract

“…GSH and SOD are oxidative stress biomarkers that are required for testes integrity (H. Yang et al, 2011). Female rats exposed to GA3 (0.2 g/L) had lower GSH levels in their progeny (Troudi, Ben Amara, et al, 2011). When compared to the GA3 group, the chrysin‐treated group showed a significant rise in catalase and GSH levels.…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress plays a role in the pathophysiology of GA3‐induced testicular injury (Troudi, Ben Amara, et al, 2011). In GA3‐treated rats, an increase in reactive oxygen radicals was linked to atrophy in the testicular seminiferous tubules and death in spermatocytes (Khalaf et al, 2019; Soliman et al, 2021b).…”

Section: Discussionmentioning

confidence: 99%

Chrysin abrogates gibberellic acid‐induced testicular oxidative stress and dysfunction via the regulation of antioxidants and steroidogenesis‐ and apoptosis‐associated genes

Soliman

Aldhahrani

Ghamry

et al. 2022

Journal of Food Biochemistry

View full text Add to dashboard Cite

GA3 is widely used as a growth stimulant in agricultural regions. The long‐term use of GA3 can cause organs damage. Chrysin is a flavonoid found in nature that is commonly used to treat organ toxicity. In this study, we examined the effect of chrysin on the testes function of GA3‐affected rats. A total of 24 male Wistar rats were divided into 4 groups. Saline was given to the control group. The chrysin group was given orally 50 mg/kg/BW of chrysin in saline. The GA3 group received a daily oral gavage of GA3 (55 mg/kg/BW). The protective group (chrysin + GA3) was given chrysin and GA3 as those described in chrysin and GA3 groups. There were an increase in MDA levels in the serum and testicular tissue of GA3‐treated group. Catalase, GSH, and SOD levels were all lowered in the GA3‐treated rats. Chrysin dramatically reduced the harmful effects of GA3 by restoring reproductive hormone levels, altered sperm parameters, and antioxidant capabilities. Furthermore, GA3 reduced the quantitative expression of steroidogenesis genes StAR and 3‐HSD, as well as Bcl2 genes, while it increased the apoptotic marker BAX; all were alleviated by the pre‐administration of chrysin. The pre‐administration of chrysin protected the GA3 group from spermatogenic vacuolation, interstitial edema, necrosis, and depletion. Chrysin inhibited oxidative stress and modulated antioxidant activity, as well as apoptosis‐/anti‐apoptosis‐related mediators in the testes. Chrysin has the potential to repair GA3‐induced testicular dysfunctions. This suggests that chrysin is preferable as a medication to mitigate GA3‐induced oxidative damage in the testes. Practical applications Chrysin has the potential to repair GA3‐induced testicular dysfunctions. This suggests that chrysin is preferable as a medication to mitigate GA3‐induced oxidative damage in the testes.

show abstract

Sorption specificity and desorption hysteresis of gibberellic acid on ferrihydrite compared to goethite, hematite, montmorillonite, and kaolinite

Zhang

Liu

Chen

2017

Environ Sci Pollut Res

View full text Add to dashboard Cite

The pesticide gibberellic acid (GA) is a potential endocrine disruptor and environmental toxin; therefore, research into its environmental fate is warranted. Batch studies were conducted to investigate the sorption and desorption characteristics of GA on aquifer media. The results demonstrated special sorption characteristic of GA on ferrihydrite compared to goethite, hematite, montmorillonite, and kaolinite, where the sorption kinetics of GA on ferrihydrite was fitted well with the pseudo-second-order, Elovich, and intra-particle diffusion models. The sorption kinetics of GA on ferrihydrite indicated an initial high sorption rate followed by a slow reaction process. The initial high GA sorption rate may be related to electrostatic sorption and surface complexation reactions on the outer surfaces and at the macropore entrances of ferrihydrite. While the slow step was controlled by GA diffusion into mesopore of ferrihydrite. Analysis of the desorption hysteresis indicated a high hysteresis index (HI) ranging from 0.68 to 17.32, and a low desorption percentage ranging from 18 to 48%. After sufficient desorption, the calculated maximum residual GA quantity due to surface complexation reactions with the ferrihydrite coordinated unsaturated sites was 9.05 ± 0.12 mg g. The calculated maximum quantity of GA trapped within the mesopore was 16.23 ± 0.91 mg g. Graphical Abstract Schematic overview of GA sorption and desorption on five minerals in groundwater.

show abstract

Oxidative stress induced by gibberellic acid in bone of suckling rats

Cited by 10 publications

References 39 publications

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

Chrysin abrogates gibberellic acid‐induced testicular oxidative stress and dysfunction via the regulation of antioxidants and steroidogenesis‐ and apoptosis‐associated genes

Sorption specificity and desorption hysteresis of gibberellic acid on ferrihydrite compared to goethite, hematite, montmorillonite, and kaolinite

Contact Info

Product

Resources

About