Purpose: This research was carried out to identify the skin antiaging effect of Psoraleae fructus extract on HaCaT cells. Methods: To investigate anti-aging effects of Psoraleae fructus on HaCaT cells, we measured cell viability, mRNA expression, and hyaluronic acid secretion. Results: In this study, we elucidated the effects of Psoraleae fructus extract on AQP3, HAS2, KRT1, and KRT10 mRNA expression and hyaluronic acid production. Real-time RT-PCR showed that Psoraleae fructus extract increased mRNA level of AQP3, HAS2, KRT1, and KRT10 gene and HA-ELISA assay also revealed that Psoraleae fructus increased HA production in HaCaT cells. Conclusion: Therefore, we successfully identified the anti-aging effect of Psoraleae fructus extract, and this result showed that the Psoraleae fructus extract can be a considerable potent cosmetic ingredient for skin moisturizing and anti-aging. Based on this, we anticipated further researches will study mechanisms of Psoraleae fructus extract to develop not only cosmetics but healthcare food or medicines.