Oxidative stress in the haematopoietic niche regulates the cellular immune response in <i>Drosophila</i>

Sinenko, Sergey; Shim, Jiwon; Banerjee, Utpal

doi:10.1038/embor.2011.223

Cited by 113 publications

(164 citation statements)

References 37 publications

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“…Similar observations were made in col larvae (10,12) and it was shown that wasp infection causes an increase in NF-κB signaling (30), ROS levels and Eiger expression in the PSC (14), leading to lamellocyte differentiation. Thus, the PSC seems required as a relay for mounting the cellular immune response to parasitic wasp infestation.…”

Section: Discussionsupporting

confidence: 79%

“…It was thus proposed that the PSC provides a microenvironment that supports hematopoietic progenitor maintenance in the LG (10,11). In line with this model, increasing or decreasing PSC size/activity can, respectively, impede or promote hemocyte differentiation (11,(13)(14)(15)(16)(17)(18)(19). However, differentiated hemocytes are frequently observed close to the PSC, and it is unclear how progenitor fate is maintained in the posterior lobes, which are far apart and physically separated from the PSC.…”

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confidence: 99%

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The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche

Benmimoun

Polesello

Haenlin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

149

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The maintenance of stem or progenitor cell fate relies on intrinsic factors as well as local cues from the cellular microenvironment and systemic signaling. In the lymph gland, an hematopoietic organ in Drosophila larva, a group of cells called the Posterior Signaling Centre (PSC), whose specification depends on the EBF transcription factor Collier (Col) and the HOX factor Antennapedia (Antp), has been proposed to form a niche required to maintain the pool of hematopoietic progenitors (prohemocytes). In contrast with this model, we show here that genetic ablation of the PSC does not cause an increase in blood cell differentiation or a loss of blood cell progenitors. Furthermore, although both col and Antp mutant larvae are devoid of PSC, the massive prohemocyte differentiation observed in col mutant is not phenocopied in Antp mutant. Interestingly, beside its expression in the PSC, Col is also expressed at low levels in prohemocytes and we show that this expression persists in PSC-ablated and Antp mutant larvae. Moreover, targeted knockdown and rescue experiments indicate that Col expression is required in the prohemocytes to prevent their differentiation. Together, our findings show that the PSC is dispensable for blood cell progenitor maintenance and reveal the key role of the conserved transcription factor Col as an intrinsic regulator of hematopoietic progenitor fate.hematopoiesis | Drosophila | stem cell niche | EBF

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Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 99%

The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche

Benmimoun

Polesello

Haenlin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

149

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“…The importance of a certain level of ROS production during differentiation has been discussed, and specific roles in regulating stem cell activity in hematopoietic stem cell niches have been shown in mammals (Simon and Keith, 2008). Moreover, detailed studies in Drosophila have revealed an important role for ROS production in the induction of lamellocyte differentiation (Owusu-Ansah and Banerjee, 2009;Sinenko et al, 2012). In P. leniusculus, ROS production is primarily regulated in the APC, and recent experiments have shown that this ROS production is associated with differentiation, affecting the structure of the extracellular matrix surrounding the hematopoietic precursors (Junkunlo, 2015).…”

Section: The Anterior Proliferation Center (Apc) Is An Important Partmentioning

confidence: 99%

Crustacean hematopoiesis

Söderhäll

2016

Developmental & Comparative Immunology

164

138

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Crustacean hemocytes are important mediators of immune reactions, and the regulation of hemocyte homeostasis is of utmost importance for the health of these animals. This review discusses the current knowledge on the lineages, synthesis and differentiation of hemocytes in crustaceans. Hematopoietic tissues, their origins, and the regulation of hematopoiesis during molting, seasonal variation and infection are discussed. Furthermore, studies concerning the molecular regulation of hemocyte formation in crustaceans are also described, and the different lineages and their molecular markers are discussed and compared with several insect species. Signaling pathways and the regulation of hematopoiesis by transcription factors are typically conserved among these arthropods, whereas cytokines and growth factors are more variable and species specific. However, considering the great diversity among the crustaceans, one should be cautious in drawing general conclusions from studies of only a few species.2

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“…In Drosophila, high ROS levels were detected in hematopoietic progenitor cells, and increased ROS levels were detected in the posterior signaling center of the lymph gland after a wasp infestation (23). In the fly, ROS serve as an important signal for progenitor cell differentiation into lamellocytes, a hemocyte type that encapsulates wasp eggs (24,25). In crayfish, a high level of ROS was detected in the APC, where actively proliferating cells are located (6).…”

mentioning

confidence: 99%

Reactive Oxygen Species Affect Transglutaminase Activity and Regulate Hematopoiesis in a Crustacean

Junkunlo

Söderhäll

et al. 2016

Journal of Biological Chemistry

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Reactive oxygen species (ROS) serve as a prime signal in the commitment to hematopoiesis in both mammals and Drosophila. In this study, the potential function of ROS during hematopoiesis in the crayfish Pacifastacus leniusculus was examined. The antioxidant N-acetylcysteine (NAC) was used to decrease ROS in both in vivo and in vitro experiments. An increase in ROS was observed in the anterior proliferation center (APC) after LPS injection. In the absence of NAC, the LPS-induced increase in ROS levels resulted in the rapid restoration of the circulating hemocyte number. In the presence of NAC, a delay in the recovery rate of the hemocyte number was observed. NAC treatment also blocked the spread of APC and other hematopoietic tissue (HPT) cells, maintaining these cells at an undifferentiated stage. Extracellular transglutaminase (TGase) has been shown previously to play a role in maintaining HPT cells in an undifferentiated form. In this study, we show that extracellular TGase activity increased when the ROS level in HPT or APC cells was reduced after NAC treatment. In addition, collagen, a major component of the extracellular matrix and a TGase substrate were co-localized on the HPT cell surface. Taken together, the results of this study show that ROS are involved in crayfish hematopoiesis, in which a low ROS level is required to maintain hematopoietic progenitor cells in the tissue and to reduce hemocyte release. The potential roles of TGase in this process are investigated and discussed.

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Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila

Cited by 113 publications

References 37 publications

The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche

The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche

Crustacean hematopoiesis

Reactive Oxygen Species Affect Transglutaminase Activity and Regulate Hematopoiesis in a Crustacean

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