Oxidative stress in systemic lupus erythematosus and rheumatoid arthritis patients: relationship to disease manifestations and activity

Hassan, Samia Z.; Gheita, Tamer A.; Kenawy, Sanaa A.; Fahim, Atef T.; El-Sorougy, Iman M.; Abdou, Manal S.

doi:10.1111/j.1756-185x.2011.01630.x

Cited by 139 publications

(85 citation statements)

References 49 publications

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“…In fact elevated MDA levels and reduced TAS levels also point towards a state of oxidative stress. Shah et al [9] have recently reported inflammatory response in SLE which aggravates oxidative stress with potential to provoke damage to lipids, proteins and DNA [12,13]. The observations of the present study on significantly increased genomic damage also find reflections in the results documented by Montalvao et al [13] who reported 1.3x significantly increased (p=0.000) DNA damage index in 25 SLE patients compared to the value in equal number of healthy controls.…”

Section: Discussionsupporting

confidence: 80%

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DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Tung

Gandhi

2015

Int Jour of Biomed Res

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Objectives: Systemic Lupus Erythematosus (SLE) is a complex, multisystem autoimmune inflammatory disease. The inflammatory response in SLE exacerbates oxidative stress which tends to promote lipid peroxidation, protein oxidation and damage to DNA. Documentation of manifestation of DNA damage in SLE patients is scarce and related studies have not come to attention from this part of the world, therefore the present study showcases a case report on SLE. Methods: Assessment of DNA damage in peripheral blood leukocytes and oxidative stress biomarkers in blood sera of a 12y-old male presenting with SLE and nephropathy (on dialysis therapy) was made. Results: Basal DNA damage in PBL scored as percent DNA in tail was higher (51.48%) compared to levels (40.38%) in an age-and sex-matched healthy control. Quantitative measures of DNA damage revealed DF of 96 vs. 94 and DI of 317 vs. 208 in the case and the control, respectively. Serum lipid peroxidation as estimated from MDA level was 3x higher (1.759µmol/l) compared to its level in a healthy control (0.571µmol/l). OSI was slightly higher in the patient (0.134 arbitrary units) compared to that in the control (0.132 arbitrary units). The atherogenic indices were higher in the present case compared to ratios in the control. Conclusion:The results from the case report on increased oxidative stress, dyslipidemia, genetic damage and atherogenic indices support observations from earlier studies and imply onset of other complications in patients with SLE. The assessment of various blood-based biomarkers (as carried out in the present case) in SLE patients may assist in disease diagnosis, prognosis and optimal disease management.

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Section: Discussionsupporting

confidence: 80%

“…The inflammatory response in SLE exacerbates oxidative stress [9] which tends to promote cellular macromolecular damage, lipid peroxidation and protein oxidation [10,11] and damage to DNA [12,13]. Health can further be compromised as any unrepaired DNA damage can initiate neoplasia [14].…”

Section: Introductionmentioning

confidence: 99%

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Tung

Gandhi

2015

Int Jour of Biomed Res

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“…Similarly, increasing evidence indicates that nitric oxide is a key regulator of apoptosis in RA [40]. Oxidative stress was reported to be increased and more profound in RA than SLE and could well reflect disease activity [11]. Increased nitric oxide levels have also been observed in HT patients [10].…”

Section: Discussionmentioning

confidence: 90%

“…Markers of oxidative stress are well correlated with disease activity in both SLE and RA [11]. NOS inhibition was reported to decrease the disease activity in experimental RA [12].…”

Section: Introductionmentioning

confidence: 99%

Influence of Endothelial Nitric Oxide Synthase Gene Intron-4 27bp Repeat Polymorphism on Its Expression in Autoimmune Diseases

AlFadhli

2013

Disease Markers

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Abstract. The purpose of this study was to analyse the effect of the T-786C polymorphism and intron 4 27 bp variable number tandem repeat(VNTR) eNOS markers for their potential association with Systemic Lupus Erythematosus(SLE), Hashimotos thyroiditis(HT) and Rheumatoid arthritis(RA) as well as to explore their effect on eNOS mRNA expression and nitrate production (NOx). Kuwaitis (n = 383) matched by age, gender and ethnicity were genotyped by fluorescent-labelled-restriction fragment length polymorphism (RFLP) and fragment analysis. Expression of eNOS mRNA was analysed using RT-PCR and sera from subjects were screened for NOx using ELISA. Analysis of the allelic frequency revealed a significant association of the 4b allele with susceptibility to SLE (p = 0.0092, OR = 1.76). The 4bb genotype was found to be associated with SLE (p = 0.0076, OR = 1.97) and HT (p = 0.05, OR = 1.81). Allelic and genotypic distribution did not differ between RA patients and healthy control subjects. The 4bb genotype resulted in reduced expression of eNOS mRNA in SLE, RA and HT, but only the reduction in HT was significant (p = 0.05). The 4ab genotype revealed a significant association with increased eNOS expression in HT (p = 0.03) and RA (p = 0.014) patients, and elevated NOx levels were detected in the autoimmune disease cohorts (p < 0.05) when compared to healthy control subjects. The T-786C SNP failed to show a significant association (p > 0.05) with SLE, HT, and RA patients. This study is the first to reveal a significant association between the 4bb genotype of the 27 bp VNTR and susceptibility to HT. The expression of eNOS is related to the number of 27 bp repeats, with heterozygous 4bb repeats showing a decrease in eNOS expression. eNOS -endothelial nitric oxide synthase.

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“…Microbiota might have different mechanisms of action over the host balancing anti-and pro-inflammatory responses (10). In line with this, even though it is not clear if oxidative stress is a cause or a consequence of this pathology; recent studies have reported higher levels of the lipid peroxidation subproduct, malondialdehyde (MDA), in lupus patients (11)(12)(13)(14). As both, oxidative stress and inflammation, may be implicated in SLE pathogenesis, they may be affected by the intake of oxidants and antioxidants and the antioxidant capacity from serum (15).…”

Section: Introductionmentioning

confidence: 76%

Microbiota and oxidant-antioxidant balance in systemic lupus erythematosus

et al. 2017

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ResumenBackground: Systemic lupus erythematosus (SLE) is a chronic infl ammatory disease of autoimmune nature, in which oxidative stress is implicated. Aim: Compare the concentrations of dietary and blood antioxidants, as well as gut microbiota, with serum malondialdehyde (MDA) and C reactive protein (CRP) in 21 subjects suffering from non-active systemic lupus erythematosus (SLE) and 21 age and gender-matched controls. Methods: General biochemical parameters and CRP were determined by enzymatic methods: copper, zinc and selenium by inductively coupled plasma mass spectrometry (ICP-MS), MDA and total antioxidant capacity (TAC) by spectrophotometric methods, gut microbiota by metagenomic analyses and dietary intake by means of food frequency questionnaire. Results: No signifi cant differences were found in diet between lupus patients and the control group, with the exception of trans fatty acids intake, which was higher in patients. In addition, higher concentration of serum copper and lower of zinc in SLE were found. Serum copper was positively associated with CRP and also, this protein with the proportion of Lentisphaerae, Proteobacteria and Verrucomicrobia in feces. Moreover, MDA levels displayed inverse correlations with the Cyanobacteria and Firmicutes groups, while Actinobacteria showed a positive association. The lupus subjects with presence of anti-SSA/Ro were related to higher levels of serum zinc. Conclusion: These results could be useful in the future to go deeper into the understanding of this complex disease. AbstractIntroducción: el lupus eritematoso sistémico es una enfermedad infl amatoria crónica en la que está implicado el estrés oxidativo. Objetivo: evaluar la concentración de antioxidantes de la dieta y sanguíneos, así como de la microbiota sobre las concentraciones de malondialdehído y proteína C reactiva en 21 pacientes de lupus y 21 controles pareados por edad y sexo. Métodos: los parámetros bioquímicos de rutina y proteína C reactiva se determinaron a través de métodos enzimáticos: cobre, zinc y selenio por espectrometría de masas, malondialdehído y capacidad antioxidante total por métodos espectrofotométricos, la microbiota fecal por técnicas metagenómicas y la dieta a través de cuestionarios de frecuencia de consumo. Resultados: no se han observado diferencias en la dieta en los pacientes con lupus respecto al grupo control, excepto en la ingesta de ácidos grasos trans, siendo mayor en el grupo de lupus. En estas pacientes se observaron mayores niveles circulantes de cobre y menores de zinc. La concentración de cobre en suero se relacionó directamente con los niveles de proteína C reactiva y esta proteína, a su vez, con la proporción de Lentisphaerae, Proteobacteria y Verrucomicrobia en heces. Además, mientras que los niveles de malondialdehído se asociaban inversamente con la proporción de Cyanobacteria y Firmicutes, con Actinobacteria se encontró una correlación positiva. La presencia de anti-SSA/Ro en lúpicas se relaciona con mayores concentraciones de zinc. Conclusió...

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Oxidative stress in systemic lupus erythematosus and rheumatoid arthritis patients: relationship to disease manifestations and activity

Cited by 139 publications

References 49 publications

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Influence of Endothelial Nitric Oxide Synthase Gene Intron-4 27bp Repeat Polymorphism on Its Expression in Autoimmune Diseases

Microbiota and oxidant-antioxidant balance in systemic lupus erythematosus

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