Oxidative stress in patients with laryngeal carcinoma

Dwivedi, RC; Raturi, DP; Kandpal, N; Rp, Singh; Vn, Puri

doi:10.4103/0019-509x.44064

Cited by 12 publications

(9 citation statements)

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“…However, numerous studies demonstrated that oxidative stress directly or indirectly causes DNA damage, which is a predisposing factor to malignant lesions (14,15). The body has established appropriate self-defense mechanisms to cope with these harmful stimuli, including the KEAP1-NRF2-AREs pathway (16). KEAP1 was discovered in 1999 (17), and the KEAP1 system is constantly researched, particularly NRF2.…”

Section: Discussionmentioning

confidence: 99%

Expression and correlation of NRF2, KEAP1, NQO-1 and HO-1 in advanced squamous cell carcinoma of the larynx and their association with clinicopathologic features

Wang

et al. 2016

Molecular Medicine Reports

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The present study aimed specifically investigate the expression and correlation of kelch-like ECH-associated protein-1 (KEAP1), nuclear factor (erythroid-derived 2)-like 2 (NRF2), quinone oxidoreductase-1 (NQO-1) and heme oxygenase-1 (HO-1) in laryngeal cancer and their association with clinicopathological features. A total of 33 paired human fresh advanced laryngeal cancer and adjacent normal specimens were collected following total laryngectomy. Immunohistochemical and immunoblotting analysis were performed to evaluate the expression of KEAP1, NRF2, NQO-1 and HO-1. All of the patients clinicopathologic features were collected. Immunohistochemistry indicated that NRF2-positive staining was as high as 79% (26/33) in the cancer samples and predominantly located in the nuclei, whereas positive expression of KEAP1, NQO-1 and HO-1 was detected in 70% (23/33), 76% (25/33) and 82% (27/33) of cancer tissues, respectively, and primarily expressed in the cytoplasm. The corresponding adjacent normal samples produced almost no or weak expression of the proteins. There were significant differences in protein expression between the two groups (P<0.01). Immunoblotting analysis also demonstrated that their expression levels were higher in cancer tissue compared with adjacent normal tissue. Notably, KEAP1, NQO-1 and HO-1 expression was positively correlated with nuclear NRF2 in cancer tissues, whereas they had no correlation with age, tumor stage (clinical stage III and IV), tumor size and lymph node metastasis. In conclusion, increased expression of NRF2, KEAP1, NQO-1 and HO-1 were common in advanced laryngeal cancer. Evaluation of these factors may have important clinical significance for the diagnosis and treatment of this disease.

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Section: Discussionmentioning

confidence: 99%

Expression and correlation of NRF2, KEAP1, NQO-1 and HO-1 in advanced squamous cell carcinoma of the larynx and their association with clinicopathologic features

Wang

et al. 2016

Molecular Medicine Reports

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“…More than a half-million new cases of head and neck cancers are reported annually worldwide [2]. Larynx squamous cell carcinoma (LSCC) constitutes almost 2% to 3% of all malignant tumours, representing the second most common malignant neoplasm of the respiratory tract [3].…”

Section: Introductionmentioning

confidence: 99%

14-3-3epsiloncontributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion

Che

Chen

et al. 2010

BMC Cancer

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Background14-3-3epsilon regulates a wide range of biological processes, including cell cycle control, proliferation, and apoptosis, and plays a significant role in neurogenesis and the formation of malignant tumours. However, the exact function and regulatory mechanism of 14-3-3epsilon in carcinogenesis have not been elucidated.MethodsThe expression of 14-3-3epsilon was assessed by RT-PCR and western blotting. The invasiveness and viability of Hep-2 cells were determined by the transwell migration assay and MTT assay, respectively. Cell cycle and apoptosis of Hep-2 cells were detected by flow cytometry.ResultsThe mRNA and protein expression of 14-3-3epsilon in larynx squamous cell carcinoma (LSCC) tissues were significantly lower than those in clear surgical margin tissues. Statistical analysis showed that the 14-3-3epsilon protein level in metastatic lymph nodes was lower than that in paired tumour tissues. In addition, the protein level of 14-3-3epsilon in stage III or IV tumours was significantly lower than that in stage I or II tumours. Compared with control Hep-2 cells, the percentages of viable cells in the 14-3-3epsilon-GFP and negative control GFP groups were 36.68 ± 14.09% and 71.68 ± 12.10%, respectively. The proportions of S phase were 22.47 ± 3.36%, 28.17 ± 3.97% and 46.15 ± 6.82%, and the apoptotic sub-G1 populations were 1.23 ± 1.02%, 2.92 ± 1.59% and 13.72 ± 3.89% in the control, negative control GFP and 14-3-3epsilon-GFP groups, respectively. The percentages of the apoptotic cells were 0.84 ± 0.25%, 1.08 ± 0.24% and 2.93 ± 0.13% in the control, negative control GFP and 14-3-3epsilon-GFP groups, respectively. The numbers of cells that penetrated the filter membrane in the control, negative control GFP and 14-3-3epsilon-GFP groups were 20.65 ± 1.94, 17.63 ± 1.04 and 9.1 ± 0.24, respectively, indicating significant differences among the different groups.ConclusionsDecreased expression of 14-3-3epsilon in LSCC tissues contributes to the initiation and progression of LSCC. 14-3-3epsilon can promote apoptosis and inhibit the invasiveness of LSCC.

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“…They concluded that the decrease in retinol levels in cancer could be due to the increased utilization by the affected tissues or in combating the excessive oxidative stress in circulation as a result of cancer. The results of the study conducted by R.C Dwivedi [25] and his co-workers show increased lipid peroxidation in laryngeal cancer patients as a consequence of free radical generation. According to Nicholas Wald [26] and his coworkers serum retinol levels in man have predictive value for subsequent cancer, low levels being most clearly associated with an increased risk of lung cancer and gastro intestinal tract cancers.…”

Section: Discussionmentioning

confidence: 94%

Evaluation of Serum Adenosine Deaminase and Retinol in patients with Laryngeal Cancer

Sharma

Desai

Metgudmath

2013

IJPBR

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Laryngeal cancers account for 4% of all head and neck cancers in India. The disease process has better prognosis if it is diagnosed early. In view of this the objective of the study was to assess the reliability of serum enzyme marker adenosine deaminase and non-enzymatic antioxidant retinol in laryngeal cancer patients as supportive parameters for diagnostic purpose. Materials and methods:-25 clinically and histopathologically confirmed patients of laryngeal cancer in age group of 45-65 years were included in the study and 25 healthy subjects with in the same age group served as controls. Results:-The level of serum ADA was significantly elevated in laryngeal cancer patients in comparison to controls whereas retinol levels were significantly decreased. Conclusion: - Lipid per-oxidation as a result of cancer leads to membrane damage and an increase in cytoplasmic ADA. It is also associated with a decrease in levels of non-enzymatic antioxidant retinol. These biochemical parameters may be used as supportive parameters for diagnostic purpose and may add further for prognostic information. Further studies are required on a larger sample size to explore strategies by which normal levels of anti-oxidant can be maintained by a retinol rich diet which may play a role in reducing the morbidity and mortality due to cancer.

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Oxidative stress in patients with laryngeal carcinoma

Cited by 12 publications

References 0 publications

Expression and correlation of NRF2, KEAP1, NQO-1 and HO-1 in advanced squamous cell carcinoma of the larynx and their association with clinicopathologic features

Expression and correlation of NRF2, KEAP1, NQO-1 and HO-1 in advanced squamous cell carcinoma of the larynx and their association with clinicopathologic features

14-3-3epsiloncontributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion

Evaluation of Serum Adenosine Deaminase and Retinol in patients with Laryngeal Cancer

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