14-3-3epsiloncontributes to tumour suppression in laryngeal carcinoma by affecting apoptosis and invasion

Abstract

Background14-3-3epsilon regulates a wide range of biological processes, including cell cycle control, proliferation, and apoptosis, and plays a significant role in neurogenesis and the formation of malignant tumours. However, the exact function and regulatory mechanism of 14-3-3epsilon in carcinogenesis have not been elucidated.MethodsThe expression of 14-3-3epsilon was assessed by RT-PCR and western blotting. The invasiveness and viability of Hep-2 cells were determined by the transwell migration assay and MTT assay, respectively. Cell cycle and apoptosis of Hep-2 cells were detected by flow cytometry.ResultsThe mRNA and protein expression of 14-3-3epsilon in larynx squamous cell carcinoma (LSCC) tissues were significantly lower than those in clear surgical margin tissues. Statistical analysis showed that the 14-3-3epsilon protein level in metastatic lymph nodes was lower than that in paired tumour tissues. In addition, the protein level of 14-3-3epsilon in stage III or IV tumours was significantly lower than that in stage I or II tumours. Compared with control Hep-2 cells, the percentages of viable cells in the 14-3-3epsilon-GFP and negative control GFP groups were 36.68 ± 14.09% and 71.68 ± 12.10%, respectively. The proportions of S phase were 22.47 ± 3.36%, 28.17 ± 3.97% and 46.15 ± 6.82%, and the apoptotic sub-G1 populations were 1.23 ± 1.02%, 2.92 ± 1.59% and 13.72 ± 3.89% in the control, negative control GFP and 14-3-3epsilon-GFP groups, respectively. The percentages of the apoptotic cells were 0.84 ± 0.25%, 1.08 ± 0.24% and 2.93 ± 0.13% in the control, negative control GFP and 14-3-3epsilon-GFP groups, respectively. The numbers of cells that penetrated the filter membrane in the control, negative control GFP and 14-3-3epsilon-GFP groups were 20.65 ± 1.94, 17.63 ± 1.04 and 9.1 ± 0.24, respectively, indicating significant differences among the different groups.ConclusionsDecreased expression of 14-3-3epsilon in LSCC tissues contributes to the initiation and progression of LSCC. 14-3-3epsilon can promote apoptosis and inhibit the invasiveness of LSCC.

MicroRNA-519a demonstrates significant tumour suppressive activity in laryngeal squamous cells by targeting anti-carcinoma HuR gene

“…2 Laryngeal SCC significantly impairs quality of life. 3 Despite many advances in the diagnosis and treatment of this disease, the overall survival rate (approximately 35-70 per cent) has remained unchanged over the past decades as a result of the uncontrolled recurrence of local lymph node metastasis. 4 Thus, it is necessary to find new ways to diagnose and treat laryngeal SCC.…”

YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells

“…High YWHAE expression was correlated with poor survival in hepatocellular carcinoma . YWHAE is upregulated in several types of cancers but is downregulated in lung, larynx, and gastric cancers . In gastric cancer, YWHAE acted as a tumor suppressor—its silencing induced cell proliferation, migration, and invasion through the upregulation of CDC25B and MYC .…”

YWHAE/14-3-3ε: a potential novel genetic risk factor and CSF biomarker for HIV neurocognitive impairment

“…Future post-mortem histological analysis of HIV patients would help to determine the levels of YWHAE in the brain in association to genotype. Considering that YWHAE protein is needed to prevent cell apoptosis (Figure 1) (Che et al 2010; Iskander et al 2004; Lipton 1992; Morales et al 2012b; Ushijima et al 1995), we would expected based on studies in HIV-seropositive males and CJD patients to see higher levels of YWHAE in post-mortem brains from females, compared to HIV-seronegative female brains (Gelman and Nguyen 2010; Torres et al 2012). However, based on our results we would expect to see an inverse relationship between levels of neurocognitive impair at the time of death and the level of YWHAE protein in the HIV-seropositive brains.…”